Construct: ORF TRCN0000488443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019178.1_s317c1
- DNA Barcode:
- ACCCTAATCCATCCATGCTTCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FZD6 (8323)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001164615.2 | 99.8% | 99.8% | (many diffs) |
| 2 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_003506.4 | 99.8% | 99.8% | (many diffs) |
| 3 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001164616.2 | 95.3% | 95.3% | (many diffs) |
| 4 | human | 8323 | FZD6 | frizzled class receptor 6 | NM_001317796.2 | 56.2% | 55.4% | (many diffs) |
| 5 | human | 8323 | FZD6 | frizzled class receptor 6 | NR_133921.2 | 51.7% | (many diffs) | |
| 6 | mouse | 14368 | Fzd6 | frizzled class receptor 6 | XM_011245455.2 | 57.3% | 59% | (many diffs) |
| 7 | mouse | 14368 | Fzd6 | frizzled class receptor 6 | XM_017316431.1 | 57.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 2193
- ORF length:
- 2118
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag atgtttacat ttttgttgac gtgtattttt ctacccctcc 121 taagagggca cagtctcttc acctgtgaac caattactgt tcccagatgt atgaaaatgg 181 cctacaacat gacgtttttc cctaatctga tgggtcatta tgaccagagt attgccgcgg 241 tggaaatgga gcattttctt cctctcgcaa atctggaatg ttcaccaaac attgaaactt 301 tcctctgcaa agcatttgta ccaacctgca tagaacaaat tcatgtggtt ccaccttgtc 361 gtaaactttg tgagaaagta tattctgatt gcaaaaaatt aattgacact tttgggatcc 421 gatggcctga ggagcttgaa tgtgacagat tacaatactg tgatgagact gttcctgtaa 481 cttttgatcc acacacagaa tttcttggtc ctcagaagaa aacagaacaa gtccaaagag 541 acattggatt ttggtgtcca aggcatctta agacttctgg gggacaagga tataagtttc 601 tgggaattga ccagtgtgcg cctccatgcc ccaacatgta ttttaaaagt gatgagctag 661 agtttgcaaa aagttttatt ggaacagttt caatattttg tctttgtgca actctgttca 721 cattccttac ttttttaatt gatgttagaa gattcagata cccagagaga ccaattatat 781 attactctgt ctgttacagc attgtatctc ttatgtactt cattggattt ttgctgggcg 841 atagcacagc ctgcaataag gcagatgaga agctagaact tggtgacact gttgtcctag 901 gctctcaaaa taaggcttgc accgttttgt tcatgctttt gtattttttc acaatggctg 961 gcactgtgtg gtgggtgatt cttaccatta cttggttctt agctgcagga agaaaatgga 1021 gttgtgaagc catcgagcaa aaagcagtgt ggtttcatgc tgttgcatgg ggaacaccag 1081 gtttcctgac tgttatgctt cttgctctga acaaagttga aggagacaac attagtggag 1141 tttgctttgt tggcctttat gacctggatg cttctcgcta ctttgtactc ttgccactgt 1201 gcctttgtgt gtttgttggg ctctctcttc ttttagctgg cattatttcc ttaaatcatg 1261 ttcgacaagt catacaacat gatggccgga accaagaaaa actaaagaaa tttatgattc 1321 gaattggagt cttcagcggc ttgtatcttg tgccattagt gacacttctc ggatgttacg 1381 tctatgagca agtgaacagg attacctggg agataacttg ggtctctgat cattgtcgtc 1441 agtaccatat cccatgtcct tatcaggcaa aagcaaaagc tcgaccagaa ttggctttat 1501 ttatgataaa atacctgatg acattaattg ttggcatctc tgctgtcttc tgggttggaa 1561 gcaaaaagac atgcacagaa tgggctgggt tttttaaacg aaatcgcaag agagatccaa 1621 tcagtgaaag tcgaagagta ctacaggaat catgtgagtt tttcttaaag cacaattcta 1681 aagttaaaca caaaaagaag cactataaac caagttcaca caagctgaag gtcatttcca 1741 aatccatggg aaccagcaca ggagctacag caaatcatgg cacttctgca gtagcaatta 1801 ctagccatga ttacctagga caagaaactt tgacagaaat ccaaaCCTCA CCAGAAACAT 1861 CAATGAGAGA GGTGAAAGCG GACGGAGCTA GCACCCCCAG GTTAAGAGAA CAGGACTGTG 1921 GTGAACCTGC CTCGCCAGCA GCATCCATCT CCAGACTCTC TGGGGAACAG GTCGACGGGA 1981 AGGGCCAGGC AGGCAGTGTA TCTGAAAGTG CGCGGAGTGA AGGAAGGATT AGTCCAAAGA 2041 GTGATATTAC TGACACTGGC CTGGCACAGA GCAACAATTT GCAGGTCCCC AGTTCTTCAG 2101 AACCAAGCAG CCTCAAAGGT TCCACATCTC TGCTTGTTCA CCCAGTTTCA GGAGTGAGAA 2161 AAGAGCAGGG AGGTGGTTGT CATTCAGATA CTGACCCAGC TTTCTTGTAC AAAGTGGTTG 2221 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2281 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 2341 CCTAATCCAT CCATGCTTCA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2401 ttgtgaaaga tt