Construct: ORF TRCN0000488453
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021391.1_s317c1
- DNA Barcode:
- TCACTGCCTATTCTCTGGCAAGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SLC2A1 (6513)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488453
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6513 | SLC2A1 | solute carrier family 2 mem... | NM_006516.3 | 99.4% | 100% | 1476_1477insTGAAAGCT |
| 2 | mouse | 20525 | Slc2a1 | solute carrier family 2 (fa... | NM_011400.3 | 89% | 97.1% | (many diffs) |
| 3 | mouse | 20525 | Slc2a1 | solute carrier family 2 (fa... | XM_006502908.1 | 88.2% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1542
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaacaat 61 tgaccatgga gcccagcagc aagaagctga cgggtcgcct catgctggcc gtgggaggag 121 cagtgcttgg ctccctgcag tttggctaca acactggagt catcaatgcc ccccagaagg 181 tgatcgagga gttctacaac cagacatggg tccaccgcta tggggagagc atcctgccca 241 ccacgctcac cacgctctgg tccctctcag tggccatctt ttctgttggg ggcatgattg 301 gctccttctc tgtgggcctt ttcgttaacc gctttggccg gcggaattca atgctgatga 361 tgaacctgct ggccttcgtg tccgccgtgc tcatgggctt ctcgaaactg ggcaagtcct 421 ttgagatgct gatcctgggc cgcttcatca tcggtgtgta ctgcggcctg accacaggct 481 tcgtgcccat gtatgtgggt gaagtgtcac ccacagccct tcgtggggcc ctgggcaccc 541 tgcaccagct gggcatcgtc gtcggcatcc tcatcgccca ggtgttcggc ctggactcca 601 tcatgggcaa caaggacctg tggcccctgc tgctgagcat catcttcatc ccggccctgc 661 tgcagtgcat cgtgctgccc ttctgccccg agagtccccg cttcctgctc atcaaccgca 721 acgaggagaa ccgggccaag agtgtgctaa agaagctgcg cgggacagct gacgtgaccc 781 atgacctgca ggagatgaag gaagagagtc ggcagatgat gcgggagaag aaggtcacca 841 tcctggagct gttccgctcc cccgcctacc gccagcccat cctcatcgct gtggtgctgc 901 agctgtccca gcagctgtct ggcatcaacg ctgtcttcta ttactccacg agcatcttcg 961 agaaggcggg ggtgcagcag cctgtgtatg ccaccattgg ctccggtatc gtcaacacgg 1021 ccttcactgt cgtgtcgctg tttgtggtgg agcgagcagg ccggcggacc ctgcacctca 1081 taggcctcgc tggcatggcg ggttgtgcca tactcatgac catcgcgcta gcactgctgg 1141 agcagctacc ctggatgtcc tatctgagca tcgtggccat ctttggcttt gtggccttct 1201 ttgaagtggg TCCTGGCCCC ATCCCATGGT TCATCGTGGC TGAACTCTTC AGCCAGGGTC 1261 CACGTCCAGC TGCCATTGCC GTTGCAGGCT TCTCCAACTG GACCTCAAAT TTCATTGTGG 1321 GCATGTGCTT CCAGTATGTG GAGCAACTGT GTGGTCCCTA CGTCTTCATC ATCTTCACTG 1381 TGCTCCTGGT TCTGTTCTTC ATCTTCACCT ACTTCAAAGT TCCTGAGACT AAAGGCCGGA 1441 CCTTCGATGA GATCGCTTCC GGCTTCCGGC AGGGGGGAGC CAGCCAAAGT GACAAGACAC 1501 CCGAGGAGCT GTTCCATCCC CTGGGGGCTG ATTCCCAAGT GTGAAAGCTT GACCCAGCTT 1561 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1621 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1681 CTTGTGGAAA GGACGATCAC TGCCTATTCT CTGGCAAGAG ACGCGTTAAG TCgacaatca 1741 acctctggat tacaaaattt gtgaaagatt