Construct: ORF TRCN0000488482
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020801.1_s317c1
- DNA Barcode:
- ATGTTGGATAGAATCTTAACCTGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ACKR4 (51554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488482
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51554 | ACKR4 | atypical chemokine receptor 4 | NM_016557.4 | 100% | 100% | |
| 2 | human | 51554 | ACKR4 | atypical chemokine receptor 4 | NM_178445.2 | 100% | 100% | |
| 3 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | NM_145700.2 | 83.3% | 85.4% | (many diffs) |
| 4 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | XM_006511734.3 | 83.3% | 85.4% | (many diffs) |
| 5 | mouse | 252837 | Ackr4 | atypical chemokine receptor 4 | XM_006511735.3 | 83.3% | 85.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1122
- ORF length:
- 1050
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggctttg gaacagaacc agtcaacaga ttattattat gaggaaaatg 121 aaatgaatgg cacttatgac tacagtcaat atgaactgat ctgtatcaaa gaagatgtca 181 gagaatttgc aaaagttttc ctccctgtat tcctcacaat agttttcgtc attggacttg 241 caggcaattc catggtagtg gcaatttatg cctattacaa gaaacagaga accaaaacag 301 atgtgtacat cctgaatttg gctgtagcag atttactcct tctattcact ctgccttttt 361 gggctgttaa tgcagttcat gggtgggttt tagggaaaat aatgtgcaaa ataacttcag 421 ccttgtacac actaaacttt gtctctggaa tgcagtttct ggcttgtatc agcatagaca 481 gatatgtggc agtaactaaa gtccccagcc aatcaggagt gggaaaacca tgctggatca 541 tctgtttctg tgtctggatg gctgccatct tgctgagcat accccagctg gttttttata 601 cagtaaatga caatgctagg tgcattccca ttttcccccg ctacctagga acatcaatga 661 aagcattgat tcaaatgcta gagatctgca ttggatttgt agtacccttt cttattatgg 721 gggtgtgcta ctttatcaca gcaaggacac tcatgaagat gccaaacatt aaaatatctc 781 gaccccTAAA AGTTCTGCTC ACAGTCGTTA TAGTTTTCAT TGTCACTCAA CTGCCTTATA 841 ACATTGTCAA GTTCTGCCGA GCCATAGACA TCATCTACTC CCTGATCACC AGCTGCAACA 901 TGAGCAAACG CATGGACATC GCCATCCAAG TCACAGAAAG CATCGCACTC TTTCACAGCT 961 GCCTCAACCC AATCCTTTAT GTTTTTATGG GAGCATCTTT CAAAAACTAC GTTATGAAAG 1021 TGGCCAAGAA ATATGGGTCC TGGAGAAGAC AGAGACAAAG TGTGGAGGAG TTTCCTTTTG 1081 ATTCTGAGGG TCCTACAGAG CCAACCAGTA CTTTTAGCAT TTAGGACCCA GCTTTCTTGT 1141 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1201 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1261 GAAAGGACGA ATGTTGGATA GAATCTTAAC CTGAACGCGT TAAGTCgaca atcaacctct 1321 ggattacaaa atttgtgaaa gatt