Construct: ORF TRCN0000488484
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020582.1_s317c1
- DNA Barcode:
- GCGGTACCCGACCAATTTCCGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- STK35 (140901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488484
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140901 | STK35 | serine/threonine kinase 35 | NM_080836.4 | 75% | 75% | 1_399del |
| 2 | human | 140901 | STK35 | serine/threonine kinase 35 | XM_011529174.3 | 30.6% | 27.5% | (many diffs) |
| 3 | mouse | 67333 | Stk35 | serine/threonine kinase 35 | NM_183262.3 | 68.7% | 70.6% | (many diffs) |
| 4 | mouse | 67333 | Stk35 | serine/threonine kinase 35 | NM_001038635.2 | 29.9% | 26.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1275
- ORF length:
- 1203
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaaacg gggaaggacg gcgcccgcag aggtacacaa agcccggagc 121 ggaaaaggcg aagcccagtg ccgcgggcgc ccagcacgaa gctgaggccg gcggcggcgg 181 cccgggccat ggatccggtg gcggccgagg ccccgggcga ggccttcctg gcgcggcgac 241 ggcctgaggg cggtggcggg tccgcgcggc cgcgttacag cctgttggcg gagatcgggc 301 gcggcagcta cggcgtggtt tatgaggcag tggccgggcg cagcggggcc cgggtggcgg 361 tcaagaagat ccgctgcgac gcccccgaga acgtggagct ggcgctggct gaattctggg 421 ccctcaccag cctcaagcgg cgccaccaga acgtcgtgca gtttgaggag tgcgtcctgc 481 agcgcaatgg gttagcccag cgcatgagtc acggcaacaa gagctcgcag ctttacctgc 541 gcctggtgga gacctcgctg aaaggagaaa ggatcctggg ttatgctgag gagccctgct 601 atctctggtt tgtcatggag ttctgtgaag gtggagacct gaatcagtat gtcctgtccc 661 ggaggccaga cccagccacc aacaaaagtt tcatgctaca gctgacgagc gccattgcct 721 tcctgcacaa aaaccatatt gtgcacaggg acctgaagcc agacaacatc ctcatcacag 781 agcggtctgg cacccccatc ctcaaagtgg ccgactttgg actaagcaag gtctgtgctg 841 ggctggcacc ccgaggcaaa gagggcaatc aagacaacaa aaatgtgaat gtgaataagt 901 actggctgtc ctcagcctgc ggttcggact tctacatggc TCCTGAAGTC TGGGAGGGAC 961 ACTACACAGC CAAGGCGGAC ATCTTTGCCC TGGGCATTAT CATCTGGGCA ATGATAGAAA 1021 GAATCACTTT TATTGACTCT GAGACCAAGA AGGAGCTCCT GGGGACCTAC ATTAAACAGG 1081 GGACTGAGAT CGTCCCTGTT GGTGAGGCGC TGCTAGAAAA CCCAAAGATG GAGTTGCACA 1141 TCCCCCAAAA ACGCAGGACT TCCATGTCTG AGGGGATCAA GCAGCTCTTG AAAGATATGT 1201 TAGCTGCTAA CCCACAGGAC CGGCCTGATG CCTTTGAACT TGAAACCAGA ATGGACCAGG 1261 TCACATGTGC TGCTTAAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCGGTAC CCGACCAATT 1441 TCCGCGTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt