Construct: ORF TRCN0000488496
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021390.1_s317c1
- DNA Barcode:
- TATAGGGACCACTCGTGACGTACG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TNFRSF1A (7132)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488496
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7132 | TNFRSF1A | TNF receptor superfamily me... | NM_001065.4 | 99.6% | 100% | 1365_1366insTGAGC |
| 2 | human | 7132 | TNFRSF1A | TNF receptor superfamily me... | NM_001346091.2 | 75.9% | 76.2% | 0_1ins324;1041_1042insTGAGC |
| 3 | human | 7132 | TNFRSF1A | TNF receptor superfamily me... | NM_001346092.2 | 62.6% | 57.4% | (many diffs) |
| 4 | human | 7132 | TNFRSF1A | TNF receptor superfamily me... | NR_144351.2 | 59.6% | 1_262del;813_814ins74;1558_2097delinsC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1431
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaggat 61 ccaccatggg cctctccacc gtgcctgacc tgctgctgcc actggtgctc ctggagctgt 121 tggtgggaat atacccctca ggggttattg gactggtccc tcacctaggg gacagggaga 181 agagagatag tgtgtgtccc caaggaaaat atatccaccc tcaaaataat tcgatttgct 241 gtaccaagtg ccacaaagga acctacttgt acaatgactg tccaggcccg gggcaggata 301 cggactgcag ggagtgtgag agcggctcct tcaccgcttc agaaaaccac ctcagacact 361 gcctcagctg ctccaaatgc cgaaaggaaa tgggtcaggt ggagatctct tcttgcacag 421 tggaccggga caccgtgtgt ggctgcagga agaaccagta ccggcattat tggagtgaaa 481 accttttcca gtgcttcaat tgcagcctct gcctcaatgg gaccgtgcac ctctcctgcc 541 aggagaaaca gaacaccgtg tgcacctgcc atgcaggttt ctttctaaga gaaaacgagt 601 gtgtctcctg tagtaactgt aagaaaagcc tggagtgcac gaagttgtgc ctaccccaga 661 ttgagaatgt taagggcact gaggactcag gcaccacagt gctgttgccc ctggtcattt 721 tctttggtct ttgcctttta tccctcctct tcattggttt aatgtatcgc taccaacggt 781 ggaagtccaa gctctactcc attgtttgtg ggaaatcgac acctgaaaaa gagggggagc 841 ttgaaggaac tactactaag cccctggccc caaacccaag cttcagtccc actccaggct 901 tcacccccac cctgggcttc agtcccgtgc ccagttccac cttcacctcc agctccacct 961 atacccccgg tgactgtccc aactttgcgg ctccccgcag agaggtggca ccaccctatc 1021 agggggctga ccccatcctt gcgacagccc tcgcctccga ccccatcccc aacccccttc 1081 agaagtGGGA GGACAGCGCC CACAAGCCAC AGAGCCTAGA CACTGATGAC CCCGCGACGC 1141 TGTACGCCGT GGTGGAGAAC GTGCCCCCGT TGCGCTGGAA GGAATTCGTG CGGCGCCTAG 1201 GGCTGAGCGA CCACGAGATC GATCGGCTGG AGCTGCAGAA CGGGCGCTGC CTGCGCGAGG 1261 CGCAATACAG CATGCTGGCG ACCTGGAGGC GGCGCACGCC GCGGCGCGAG GCCACGCTGG 1321 AGCTGCTGGG ACGCGTGCTC CGCGACATGG ACCTGCTGGG CTGCCTGGAG GACATCGAGG 1381 AGGCGCTTTG CGGCCCCGCC GCCCTCCCGC CCGCGCCCAG TCTTCTCAGA TGAGCTAGCG 1441 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1501 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1561 TTTATATATC TTGTGGAAAG GACGATATAG GGACCACTCG TGACGTACGA CGCGTTAAGT 1621 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt