Construct: ORF TRCN0000488523
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021359.1_s317c1
- DNA Barcode:
- AAGGACTGTATTGCCTAGTGAACC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SERPINB5 (5268)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488523
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5268 | SERPINB5 | serpin family B member 5 | NM_002639.5 | 57.8% | 48.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 765
- ORF length:
- 693
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgcc ctgcaactag caaattcggc ttttgccgtt gatctgttca 121 aacaactatg tgaaaaggag ccactgggca atgtcctctt ctctccaatc tgtctctcca 181 cctctctgtc acttgctcaa gtgggtgcta aaggtgacac tgcaaatgaa attggacagg 241 ttcttcattt tgaaaatgtc aaagatgtac cctttggatt tcaaacagta acatcggatg 301 taaacaaact tagttccttt tactcactga aactaatcaa gcggctctac gtagacaaat 361 ctctgaatct ttctacagag ttcatcagct ctacgaagag accctatgca aaggaattgg 421 aaactgttga cttcaaagat aaattGGAAG AAACGAAAGG TCAGATCAAC AACTCAATTA 481 AGGATCTCAC AGATGGCCAC TTTGAGAACA TTTTAGCTGA CAACAGTGTG AACGACCAGA 541 CCAAAATCCT TGTGGTTAAT GCTGCCTACT TTGTTGGCAA GTGGATGAAG AAATTTCCTG 601 AATCAGAAAC AAAAGAATGT CCTTTCAGAG TCAACAAGGT ATGTGGGGCA GCATGTAGCA 661 GTAAAAGGAG CCCAATTATA GATGTGAAAA ATGACAGGGA CAGAGTGGGG CATAAATCCA 721 TTCCAATGAG GAACCTGAGG GCACGGCCGG CAAAGTGCCT TTCCTAGAAC CCAGCTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAAAGGACT GTATTGCCTA GTGAACCACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt