Construct: ORF TRCN0000488524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021277.1_s317c1
- DNA Barcode:
- CCTTAAATTATGCCTCCGCCGCCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ENOPH1 (58478)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 58478 | ENOPH1 | enolase-phosphatase 1 | NM_001292017.2 | 66.4% | 66.4% | 1_174del |
| 2 | human | 58478 | ENOPH1 | enolase-phosphatase 1 | NM_021204.5 | 44% | 44% | 1_438del |
| 3 | human | 58478 | ENOPH1 | enolase-phosphatase 1 | NR_120457.2 | 18.6% | 1_446del;792_1851del | |
| 4 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | NM_001163035.1 | 37.6% | 42.2% | (many diffs) |
| 5 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | NM_026421.3 | 37.6% | 42.2% | (many diffs) |
| 6 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | XM_006535189.2 | 36.5% | 40.9% | (many diffs) |
| 7 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | XM_006535190.2 | 36.5% | 40.9% | (many diffs) |
| 8 | mouse | 67870 | Enoph1 | enolase-phosphatase 1 | XM_011249550.2 | 36.5% | 40.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 414
- ORF length:
- 345
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 CAGGCACCAT GAAGGTGTAC ATCTATTCCT CAGGGAGTGT GGAGGCACAG AAACTGTTAT 121 TCGGGCATTC TACGGAGGGA GATATTCTTG AGCTTGTTGA TGGTCACTTT GATACCAAGA 181 TTGGACACAA AGTAGAGAGT GAAAGTTACC GAAAGATTGC AGACAGCATT GGGTGCTCAA 241 CCAACAACAT TTTGTTTCTG ACAGATGTTA CTCGAGAGGC CAGTGCTGCT GAGGAAGCAG 301 ATGTGCACGT AGCTGTGGTG GTGAGACCAG GCAACGCAGG ATTAACAGAT GATGAGAAGA 361 CTTACTACAG CCTCATCACA TCCTTCAGTG AACTATACCT GCCTTCCTCA ACCTAGAACC 421 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GACCTTAAAT TATGCCTCCG CCGCCCACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt