Construct: ORF TRCN0000488530
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020874.1_s317c1
- DNA Barcode:
- GGTTCAATCAACCTAATCCATCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- HTR2C (3358)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488530
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3358 | HTR2C | 5-hydroxytryptamine recepto... | NM_000868.3 | 100% | 100% | |
| 2 | human | 3358 | HTR2C | 5-hydroxytryptamine recepto... | NM_001256760.2 | 100% | 100% | |
| 3 | human | 3358 | HTR2C | 5-hydroxytryptamine recepto... | NM_001256761.2 | 54.1% | 37.7% | 454_455ins95;744_745ins535 |
| 4 | mouse | 15560 | Htr2c | 5-hydroxytryptamine (seroto... | NM_008312.4 | 86.2% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1446
- ORF length:
- 1374
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggtgaac ctgaggaatg cggtgcattc attccttgtg cacctaattg 121 gcctattggt ttggcaatgt gatatttctg tgagcccagt agcagctata gtaactgaca 181 ttttcaatac ctccgatggt ggacgcttca aattcccaga cggggtacaa aactggccag 241 cactttcaat cgtcatcata ataatcatga caataggtgg caacatcctt gtgatcatgg 301 cagtaagcat ggaaaagaaa ctgcacaatg ccaccaatta cttcttaatg tccctagcca 361 ttgctgatat gctagtggga ctacttgtca tgcccctgtc tctcctggca atcctttatg 421 attatgtctg gccactacct agatatttgt gccccgtctg gatttcttta gatgttttat 481 tttcaacagc gtccatcatg cacctctgcg ctatatcgct ggatcggtat gtagcaatac 541 gtaatcctat tgagcatagc cgtttcaatt cgcggactaa ggccatcatg aagattgcta 601 ttgtttgggc aatttctata ggtgtatcag ttcctatccc tgtgattgga ctgagggacg 661 aagaaaaggt gttcgtgaac aacacgacgt gcgtgctcaa cgacccaaat ttcgttctta 721 ttgggtcctt cgtagctttc ttcataccgc tgacgattat ggtgattacg tattgcctga 781 ccatctacgt tctgcgccga caagctttga tgttactgca cggccacacc gaggaaccgc 841 ctggactaag tctggatttc ctgaagtgct gcaagaggaa tacggccgag gaagagaact 901 ctgcaaaccc taaccaagac cagaacgcac gccgaagaaa gaagaaggag agacgtccta 961 ggggcaccat gcaggctatc aacaatgaaa gaaaagcttc gaaagtcctt gggattgttt 1021 tctttgtgtt tctgatcatg tggtgcccat ttttcattac caatattctg tctgttcttt 1081 gtgagaagtc ctgtaaccaa aagctcatgg aaaagcttct gaatgtgttt gtttggattG 1141 GCTATGTTTG TTCAGGAATC AATCCTCTGG TGTATACTCT GTTCAACAAA ATTTACCGAA 1201 GGGCATTCTC CAACTATTTG CGTTGCAATT ATAAGGTAGA GAAAAAGCCT CCTGTCAGGC 1261 AGATTCCAAG AGTTGCCGCC ACTGCTTTGT CTGGGAGGGA GCTTAATGTT AACATTTATC 1321 GGCATACCAA TGAACCGGTG ATCGAGAAAG CCAGTGACAA TGAGCCCGGT ATAGAGATGC 1381 AAGTTGAGAA TTTAGAGTTA CCAGTAAATC CCTCCAGTGT GGTTAGCGAA AGGATTAGCA 1441 GTGTGTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1501 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1561 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGTTCA ATCAACCTAA TCCATCATAC 1621 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt