Construct: ORF TRCN0000488533
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020477.1_s317c1
- DNA Barcode:
- AGTCTGTTCGCAACAGTATGGATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAPSS2 (9060)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488533
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9060 | PAPSS2 | 3'-phosphoadenosine 5'-phos... | NM_004670.4 | 100% | 100% | |
| 2 | human | 9060 | PAPSS2 | 3'-phosphoadenosine 5'-phos... | NM_001015880.2 | 99.1% | 99.1% | 864_878del |
| 3 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | NM_001201470.1 | 86.6% | 93.1% | (many diffs) |
| 4 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | XM_006527065.2 | 86.1% | 91.8% | (many diffs) |
| 5 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | XM_006527064.3 | 85.9% | 92.4% | (many diffs) |
| 6 | mouse | 23972 | Papss2 | 3'-phosphoadenosine 5'-phos... | NM_011864.3 | 85.5% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1914
- ORF length:
- 1842
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcgggg atcaagaagc aaaagacgga gaaccagcag aaatccacca 121 atgtagtcta tcaggcccac catgtgagca ggaataagag agggcaagtg gttggaacaa 181 ggggtgggtt ccgaggatgt accgtgtggc taacaggtct ctctggtgct ggaaaaacaa 241 cgataagttt tgccctggag gagtaccttg tctcccatgc catcccttgt tactccctgg 301 atggggacaa tgtccgtcat ggccttaaca gaaatctcgg attctctcct ggggacagag 361 aggaaaatat ccgccggatt gctgaggtgg ctaagctgtt tgctgatgct ggtctggtct 421 gcattaccag ctttatttct ccattcgcaa aggatcgtga gaatgcccgc aaaatacatg 481 aatcagcagg gctgccattc tttgaaatat ttgtagatgc acctctaaat atttgtgaaa 541 gcagagacgt aaaaggcctc tataaaaggg ccagagctgg ggagattaaa ggatttacag 601 gtattgattc tgattatgag aaacctgaaa ctcctgagcg tgtgcttaaa accaatttgt 661 ccacagtgag tgactgtgtc caccaggtag tggaacttct gcaagagcag aacattgtac 721 cctatactat aatcaaagat atccacgaac tctttgtgcc ggaaaacaaa cttgaccacg 781 tccgagctga ggctgaaact ctcccttcat tatcaattac taagctggat ctccagtggg 841 tccaggtttt gagcgaaggc tgggccactc ccctcaaagg tttcatgcgg gagaaggagt 901 acttacaggt tatgcacttt gacaccctgc tagatgatgg cgtgatcaac atgagcatcc 961 ccattgtact gcccgtctct gcagaggata agacacggct ggaagggtgc agcaagtttg 1021 tcctggcaca tggtggacgg agggtagcta tcttacgaga cgctgaattc tatgaacaca 1081 gaaaagagga acgctgttcc cgtgtttggg ggacaacatg tacaaaacac ccccatatca 1141 aaatggtgat ggaaagtggg gactggctgg ttggtggaga ccttcaggtg ctggagaaaa 1201 taagatggaa tgatgggctg gaccaatacc gtctgacacc tctggagctc aaacagaaat 1261 gtaaagaaat gaatgctgat gcggtgtttg cattccagtt gcgcaatcct gtccacaatg 1321 gccatgccct gttgatgcag gacactcgcc gcaggctcct agagaggggc tacaagcacc 1381 cggtcctcct actacaccct ctgggcggct ggaccaagga tgacgatgtg cctctagact 1441 ggcggatgaa gcagcacgcg gctgtgctcg aggaaggggt cctggatccc aagtcaacca 1501 ttgttgccat ctttccgtct cccatgttat atgctggCCC CACAGAGGTC CAGTGGCACT 1561 GCAGGTCCCG GATGATTGCG GGTGCCAATT TCTACATTGT GGGGAGGGAC CCTGCAGGAA 1621 TGCCCCATCC TGAAACCAAG AAGGATCTGT ATGAACCCAC TCATGGGGGC AAGGTCTTGA 1681 GCATGGCCCC TGGCCTCACC TCTGTGGAAA TCATTCCATT CCGAGTGGCT GCCTACAACA 1741 AAGCCAAAAA AGCCATGGAC TTCTATGATC CAGCAAGGCA CAATGAGTTT GACTTCATCT 1801 CAGGAACTCG AATGAGGAAG CTCGCCCGGG AAGGAGAGAA TCCCCCAGAT GGCTTCATGG 1861 CCCCCAAAGC ATGGAAGGTC CTGACAGATT ATTACAGGTC CCTGGAGAAG AACTAGGACC 1921 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1981 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 2041 ATATATCTTG TGGAAAGGAC GAAGTCTGTT CGCAACAGTA TGGATGACGC GTTAAGTCga 2101 caatcaacct ctggattaca aaatttgtga aagatt