Construct: ORF TRCN0000488540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019232.1_s317c1
- DNA Barcode:
- TGACGTACGGCAGTTCAGACTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAMP1 (10267)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10267 | RAMP1 | receptor activity modifying... | NM_005855.4 | 99.7% | 99.3% | 444_445insG |
| 2 | human | 10267 | RAMP1 | receptor activity modifying... | NM_001308353.2 | 84.9% | 84.5% | 0_1ins66;378_379insG |
| 3 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003152.2 | 84.9% | 84.5% | 0_1ins66;378_379insG |
| 4 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003153.2 | 84.9% | 84.5% | 0_1ins66;378_379insG |
| 5 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003154.1 | 84.9% | 84.5% | 0_1ins66;378_379insG |
| 6 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003156.2 | 84.9% | 84.5% | 0_1ins66;378_379insG |
| 7 | human | 10267 | RAMP1 | receptor activity modifying... | XM_017003155.1 | 61.6% | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 522
- ORF length:
- 447
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc cgggccctgt gccgcctccc gcggcgcggc ctctggctgc 121 tcctggccca tcacctcttc atgaccactg cctgccagga ggctaactac ggtgccctcc 181 tccgggagcT CTGCCTCACC CAGTTCCAGG TAGACATGGA GGCCGTCGGG GAGACGCTGT 241 GGTGTGACTG GGGCAGGACC ATCAGGAGCT ACAGGGAGCT GGCCGACTGC ACCTGGCACA 301 TGGCGGAGAA GCTGGGCTGC TTCTGGCCCA ATGCAGAGGT GGACAGGTTC TTCCTGGCAG 361 TGCATGGCCG CTACTTCAGG AGCTGCCCCA TCTCAGGCAG GGCCGTGCGG GACCCGCCCG 421 GCAGCATCCT CTACCCCTTC ATCGTGGTCC CCATCACGGT GACCCTGCTG GTGACGGCAC 481 TGGTGGTCTG GCAGAGCAAG CGCACTGAGG GCATTGTGGA CCCAGCTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATGACGT ACGGCAGTTC AGACTTATAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt