Construct: ORF TRCN0000488547
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019618.1_s317c1
- DNA Barcode:
- ACCAACCCAAGCTACGGATAATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488547
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26497 | OR10D3 | olfactory receptor family 1... | NM_001355213.1 | 99.8% | 99.6% | 936_937insG |
| 2 | mouse | 258327 | Olfr958 | olfactory receptor 958 | NM_146330.1 | 83.7% | 88.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1014
- ORF length:
- 939
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag gtgaagaact gctgcatggt gacagagttc atccttttgg 121 gaatcccaca cacagagggg ctggagatga cactttttgt cttattcttg cccttctatg 181 cctgcactct actgggaaat gtgtctatcc ttgttgctgt tatgtcttct gctcgccttc 241 acacacctat gtatttcttc ctgggaaact tgtctgtgtt tgacatgggt ttctcctcag 301 tgacttgtcc caaaatgctg ctctacctta tggggctgag ccgactcatc tcctacaaag 361 actgtgtctg ccagcttttc ttcttccatt tcctcgggag cattgagtgc ttcttgttta 421 cggtgatggc ctatgaccgc ttcactgcca tctgttatcc tctgcgatac acagtcatca 481 tgaacccaag gatctgtgtg gccctggctg tgggcacatg gctgttaggg tgcattcatt 541 ccagtatctt gacctccctc accttcacct tgccatactg tggtcccaat gaagtggatc 601 acttcttctg tgacattcca gcactgttgc ccttggcctg tgctgacaca tccTTAGCCC 661 AGAGGGTGAG CTTCACCAAC GTTGGCCTCA TATCTCTTGT CTGCTTTCTG CTAATTCTTT 721 TATCCTACAC TAGAATCACA ATATCTATCT TAAGCATTCG TACAACTGAG GGCCGTCGCC 781 GTGCCTTCTC CACCTGCAGT GCTCACCTCA TTGCCATCCT CTGTGCCTAT GGGCCCATCA 841 TCACTGTCTA CCTGCAGCCC ACACCCAACC CCATGCTGGG AACCGTGGTA CAAATTCTCA 901 TGAATCTGGT AGGACCAATG CTGAACCCTT TGATCTATAC CTTGAGGAAT AAGGAAGTAA 961 AAACAGCCCT GAAAACAATA TTGCACAGGA CAGGCCATGT TCCTGAGAGT GACCCAGCTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGAACCA ACCCAAGCTA CGGATAATGC ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt