Construct: ORF TRCN0000488548
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019410.1_s317c1
- DNA Barcode:
- GGATCCTGTGTCCACACACGCACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPRC5D (55507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488548
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55507 | GPRC5D | G protein-coupled receptor ... | NM_018654.1 | 99.5% | 99.7% | (many diffs) |
| 2 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_005253421.3 | 99.5% | 99.7% | (many diffs) |
| 3 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019582.2 | 99.5% | 99.7% | (many diffs) |
| 4 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019583.2 | 86.8% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1110
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgtac aaggactgca tcgagtccac tggagactat tttcttctct 121 gtgacgccga ggggccatgg ggcatcattc tggagtccct ggccatactt ggcatcgtgg 181 tcacaattct gctactctta gcatttctct tcctcatgcg aaagatccaa gactgcagcc 241 agtggaatgt cctccccacc cagctcctct tcctcctgag tgtcctgggg ctcttcggac 301 tcgcttttgc cttcatcatc gagctcaatc aacaaactgc ccccgtacgc tactttctct 361 ttggggttct ctttgctctc tgtttctcat gcctcttagc tcatgcctcc aatctagtga 421 agctggttcg gggttgtgtc tccttctcct ggacgacaat tctgtgcatt gctattggtt 481 gcagtctgtt gcaaatcatt attgccactg agtatgtgac tctcatcatg accagaggta 541 tgatgtttgt gaatatgaca ccctgccagc tcaatgtgga ctttgttgta ctcctggtct 601 atgtcctctt cctgatggcc ctcacattct tcgtctccaa agccaccttc tgtggcccgt 661 gtgagaactg gaagcagcat ggaaggctca tctttatcac tgcgctcttc tccatcatca 721 tctgggtggt gtggatctcc atgctccTGA GAGGCAACCC GCAGTTCCAG CGACAGCCCC 781 AGTGGGATGA CCCGGTCGTC TGCATTGCTC TGGTCACCAA CGCATGGGTT TTCCTGCTGC 841 TGTACATCGT CCCTGAGCTC TGCATTCTCT ACAGATCGTG TAGACAGGAG TGCCCTTTAC 901 AAGGCAATGC CTGCCCCGTC ACAGCCTACC AACACAGCTT CCAAGTGGAG AACCAGGAGC 961 TCTCCAGAGC CCGAGACAGT GATGGAGCTG AGGAGGATGT AGCATTAACT TCATATGGTA 1021 CTCCCATTCA GCCGCAGACT GTTGATCCCA CACAAGAGTG CTTCATCCCA CAGGCTAAAC 1081 TAAGCCCCCA GCAAGATGCA GGAGGAGTAG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGGATC 1261 CTGTGTCCAC ACACGCACTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt