Construct: ORF TRCN0000488581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019230.1_s317c1
- DNA Barcode:
- CCTGCTTTCCACTTTTAGTTCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALR3 (8484)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8484 | GALR3 | galanin receptor 3 | NM_003614.2 | 81.4% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1179
- ORF length:
- 1104
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggcc gacgcccaga acatcagcct ggacagccct ggcagcgtgg 121 gagctgtggc tgtgcctgtg gtgttcgccc tgatcttcct gctgggcacc gtgggcaacg 181 gcctggtgct ggctgtgctg ctgcagcctg gaccatccgc ctggcaggaa cctggcagca 241 ccaccgacct gttcatcctg aacctggccg tggccgacct gtgcttcatc ctgtgctgcg 301 tgccattcca ggccaccatc tacaccctgg acgcctggct gttcggcgcc ctcgtgtgca 361 aggccgtgca tctgctgatc tacctgacta tgtacgccag ctccttcacc ctcgctgctg 421 tgtccgtgga ccggtatctg gccgtgcggc accccctgag aagcagagcc ctgcggaccc 481 ccagaaacgc cagagctgcc gtgggcctcg tgtggctgct ggccgccctg tttagcgccc 541 cctacctgag ctactacggc acagtgcgct acggcgccct ggaactgtgt gtgcccgcct 601 gggaggacgc cagacgcaga gcactggacg tggccacctt cgccgctggc tacctgctcc 661 ctgtcgccgt ggtgtctctg gcctacggcc ggaccctgcg gtttctgtgg gccgctgtgg 721 gacctgctgg cgcagcagcc gctgaagcca gaagaagggc cacaggcaga gccggcaggg 781 ctatgctggc agtggccgct ctgtacgccc tgtgttgggg acctcaccac gccctgatcc 841 tgtgcttttG GTACGGCAGA TTCGCCTTCA GCCCCGCCAC CTACGCCTGC AGACTGGCCT 901 CTCACTGCCT GGCCTACGCC AACAGCTGCC TGAACCCCCT GGTGTATGCC CTGGCCAGCC 961 GGCACTTCCG GGCCAGATTC AGACGGCTGT GGCCCTGCGG CAGACGGCGG AGACACAGAG 1021 CTAGACGGGC CCTGCGGAGA GTGCGGCCTG CCTCTTCTGG CCCTCCTGGC TGTCCTGGAG 1081 ACGCCAGACC TAGCGGCAGA CTGCTGGCTG GCGGCGGACA GGGACCCGAG CCTAGAGAAG 1141 GACCTGTGCA CGGCGGCGAG GCCGCCAGAG GACCTGAAGA CCCAGCTTTC TTGTACAAAG 1201 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1261 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1321 ACGACCTGCT TTCCACTTTT AGTTCTGGAC GCGTTAAGTC gacaatcaac ctctggatta 1381 caaaatttgt gaaagatt