Construct: ORF TRCN0000488594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021743.1_s317c1
- DNA Barcode:
- TCAACGTTTGTTTCGGCCGTGTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NEDD9 (4739)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488594
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_006403.4 | 99.9% | 100% | 141A>G |
| 2 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_001142393.1 | 99.5% | 99.6% | 4_8delTGGAC;11_12insTATAA;141A>G |
| 3 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_001271033.1 | 82% | 81.7% | (many diffs) |
| 4 | human | 4739 | NEDD9 | neural precursor cell expre... | NR_073131.1 | 50% | (many diffs) | |
| 5 | human | 4739 | NEDD9 | neural precursor cell expre... | NM_182966.4 | 20.4% | 18.8% | (many diffs) |
| 6 | mouse | 18003 | Nedd9 | neural precursor cell expre... | NM_017464.5 | 84.7% | 89.2% | (many diffs) |
| 7 | mouse | 18003 | Nedd9 | neural precursor cell expre... | NM_001111324.2 | 84.5% | 88.8% | (many diffs) |
| 8 | mouse | 18003 | Nedd9 | neural precursor cell expre... | XM_006516874.3 | 84.4% | 88.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2571
- ORF length:
- 2502
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gaagtataag aatcttatgg caagggcctt atatgacaat gtcccagagt 121 gtgccgagga actggccttt cgcaagggag acatcctgac cgtcatagag cagaacacag 181 ggggactgga aggatggtgg ctgtgctcgt tacacggtcg gcaaggcatt gtcccaggca 241 accgggtgaa gcttctgatt ggtcccatgc aggagactgc ctccagtcac gagcagcctg 301 cctctggact gatgcagcag acctttggcc aacagaagct ctatcaagtg ccaaacccac 361 aggctgctcc ccgagacacc atctaccaag tgccaccttc ctaccaaaat cagggaattt 421 accaagtccc cactggccac ggcacccaag aacaagaggt atatcaggtg ccaccatcag 481 tgcagagaag cattggggga accagtgggc cccacgtggg taaaaaggtg ataacccccg 541 tgaggacagg ccatggctac gtatacgagt acccatccag ataccaaaag gacgtctatg 601 atatccctcc ttctcatacc actcaagggg tatacgacat ccctccctca tcagcaaaag 661 gccctgtgtt ttcagttcca gtgggagaga taaaacctca aggggtgtat gacatcccgc 721 ctacaaaagg ggtatatgcc attccgccct ctgcttgccg ggatgaagca gggcttaggg 781 aaaaagacta tgacttcccc cctcccatga gacaagctgg aaggccggac ctcagaccgg 841 agggggttta tgacattcct ccaacctgca ccaagccagc agggaaggac cttcatgtaa 901 aatacaactg tgacattcca ggagctgcag aaccggtggc tcgaaggcac cagagcctgt 961 ccccgaatca cccacccccg caactcggac agtcagtggg ctctcagaac gacgcatatg 1021 atgtcccccg aggcgttcag tttcttgagc caccagcaga aaccagtgag aaagcaaacc 1081 cccaggaaag ggatggtgtt tatgatgtcc ctctgcataa cccgccagat gctaaaggct 1141 ctcgggactt ggtggatggg atcaaccgat tgtctttctc cagtacaggc agcacccgga 1201 gtaacatgtc cacgtcttcc acctcctcca aggagtcctc actgtcagcc tccccagctc 1261 aggacaaaag gctcttcctg gatccagaca cagctattga gagacttcag cggctccagc 1321 aggcccttga gatgggtgtc tccagcctaa tggcactggt cactaccgac tggcggtgtt 1381 acggatatat ggaaagacac atcaatgaaa tacgcacagc agtggacaag gtggagctgt 1441 tcctgaagga gtacctccac tttgtcaagg gagctgttgc aaatgctgcc tgcctcccgg 1501 aactcatcct ccacaacaag atgaagcggg agctgcaacg agttgaagac tcccaccaga 1561 tcctgagtca aaccagccat gacttaaatg agtgcagctg gtccctgaat atcttggcca 1621 tcaacaagcc ccagaacaag tgtgacgatc tggaccggtt tgtgatggtg gcaaagacgg 1681 tgcccgatga cgccaagcag ctcaccacaa ccatcaacac caacgcagag gccctcttca 1741 gacccggccc tggcagcttg catctgaaga atgggccgga gagcatcatg aactcaacgg 1801 agtacccaca cggtggctcc cagggacagc tgctgcatcc tggtgaccac aaggcccagg 1861 cccacaacaa ggcactgccc ccaggcctga gcaaggagca ggcccctgac tgtagcagca 1921 gtgatggttc tgagaggagc tggatggatg actacgatta cgtccaccta cagggtaagg 1981 aggagtttga gaggcaacag aaagagctat tggaaaaaga gaatatcatg aaacagaaca 2041 agatgcagct ggaacatcat cagctgagcc agttccagct gttggaacaa gagattacaa 2101 agcccgtgga gaatgacatc tcgaagtgga agccctctca gagcctaccc accacaaaca 2161 gtggcgtgag tgctcaggat cggcagttgc tgtgcttcta ctatgaccaa tgtgagaccc 2221 atttcatttc ccTTCTCAAC GCCATTGACG CACTCTTCAG TTGTGTCAGC TCAGCCCAGC 2281 CCCCGCGAAT CTTCGTGGCA CACAGCAAGT TTGTCATCCT CAGTGCACAC AAACTGGTGT 2341 TCATTGGAGA CACGCTGACA CGGCAGGTGA CTGCCCAGGA CATTCGCAAC AAAGTCATGA 2401 ACTCCAGCAA CCAGCTCTGC GAGCAGCTCA AGACCATAGT CATGGCAACC AAGATGGCCG 2461 CCCTCCATTA CCCCAGCACC ACGGCCCTGC AGGAAATGGT GCACCAAGTG ACAGACCTTT 2521 CTAGAAATGC CCAGCTGTTC AAGCGCTCTT TGCTGGAGAT GGCAACGTTC TAGAACCCAG 2581 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2641 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2701 TATCTTGTGG AAAGGACGAT CAACGTTTGT TTCGGCCGTG TCAACGCGTT AAGTCgacaa 2761 tcaacctctg gattacaaaa tttgtgaaag att