Construct: ORF TRCN0000488594

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021743.1_s317c1
DNA Barcode:
TCAACGTTTGTTTCGGCCGTGTCA
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
NEDD9 (4739)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488594
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4739 NEDD9 neural precursor cell expre... NM_006403.4 99.9% 100% 141A>G
2 human 4739 NEDD9 neural precursor cell expre... NM_001142393.1 99.5% 99.6% 4_8delTGGAC;11_12insTATAA;141A>G
3 human 4739 NEDD9 neural precursor cell expre... NM_001271033.1 82% 81.7% (many diffs)
4 human 4739 NEDD9 neural precursor cell expre... NR_073131.1 50% (many diffs)
5 human 4739 NEDD9 neural precursor cell expre... NM_182966.4 20.4% 18.8% (many diffs)
6 mouse 18003 Nedd9 neural precursor cell expre... NM_017464.5 84.7% 89.2% (many diffs)
7 mouse 18003 Nedd9 neural precursor cell expre... NM_001111324.2 84.5% 88.8% (many diffs)
8 mouse 18003 Nedd9 neural precursor cell expre... XM_006516874.3 84.4% 88.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2571
ORF length:
2502
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gaagtataag aatcttatgg caagggcctt atatgacaat gtcccagagt
121gtgccgagga actggccttt cgcaagggag acatcctgac cgtcatagag cagaacacag
181ggggactgga aggatggtgg ctgtgctcgt tacacggtcg gcaaggcatt gtcccaggca
241accgggtgaa gcttctgatt ggtcccatgc aggagactgc ctccagtcac gagcagcctg
301cctctggact gatgcagcag acctttggcc aacagaagct ctatcaagtg ccaaacccac
361aggctgctcc ccgagacacc atctaccaag tgccaccttc ctaccaaaat cagggaattt
421accaagtccc cactggccac ggcacccaag aacaagaggt atatcaggtg ccaccatcag
481tgcagagaag cattggggga accagtgggc cccacgtggg taaaaaggtg ataacccccg
541tgaggacagg ccatggctac gtatacgagt acccatccag ataccaaaag gacgtctatg
601atatccctcc ttctcatacc actcaagggg tatacgacat ccctccctca tcagcaaaag
661gccctgtgtt ttcagttcca gtgggagaga taaaacctca aggggtgtat gacatcccgc
721ctacaaaagg ggtatatgcc attccgccct ctgcttgccg ggatgaagca gggcttaggg
781aaaaagacta tgacttcccc cctcccatga gacaagctgg aaggccggac ctcagaccgg
841agggggttta tgacattcct ccaacctgca ccaagccagc agggaaggac cttcatgtaa
901aatacaactg tgacattcca ggagctgcag aaccggtggc tcgaaggcac cagagcctgt
961ccccgaatca cccacccccg caactcggac agtcagtggg ctctcagaac gacgcatatg
1021atgtcccccg aggcgttcag tttcttgagc caccagcaga aaccagtgag aaagcaaacc
1081cccaggaaag ggatggtgtt tatgatgtcc ctctgcataa cccgccagat gctaaaggct
1141ctcgggactt ggtggatggg atcaaccgat tgtctttctc cagtacaggc agcacccgga
1201gtaacatgtc cacgtcttcc acctcctcca aggagtcctc actgtcagcc tccccagctc
1261aggacaaaag gctcttcctg gatccagaca cagctattga gagacttcag cggctccagc
1321aggcccttga gatgggtgtc tccagcctaa tggcactggt cactaccgac tggcggtgtt
1381acggatatat ggaaagacac atcaatgaaa tacgcacagc agtggacaag gtggagctgt
1441tcctgaagga gtacctccac tttgtcaagg gagctgttgc aaatgctgcc tgcctcccgg
1501aactcatcct ccacaacaag atgaagcggg agctgcaacg agttgaagac tcccaccaga
1561tcctgagtca aaccagccat gacttaaatg agtgcagctg gtccctgaat atcttggcca
1621tcaacaagcc ccagaacaag tgtgacgatc tggaccggtt tgtgatggtg gcaaagacgg
1681tgcccgatga cgccaagcag ctcaccacaa ccatcaacac caacgcagag gccctcttca
1741gacccggccc tggcagcttg catctgaaga atgggccgga gagcatcatg aactcaacgg
1801agtacccaca cggtggctcc cagggacagc tgctgcatcc tggtgaccac aaggcccagg
1861cccacaacaa ggcactgccc ccaggcctga gcaaggagca ggcccctgac tgtagcagca
1921gtgatggttc tgagaggagc tggatggatg actacgatta cgtccaccta cagggtaagg
1981aggagtttga gaggcaacag aaagagctat tggaaaaaga gaatatcatg aaacagaaca
2041agatgcagct ggaacatcat cagctgagcc agttccagct gttggaacaa gagattacaa
2101agcccgtgga gaatgacatc tcgaagtgga agccctctca gagcctaccc accacaaaca
2161gtggcgtgag tgctcaggat cggcagttgc tgtgcttcta ctatgaccaa tgtgagaccc
2221atttcatttc ccTTCTCAAC GCCATTGACG CACTCTTCAG TTGTGTCAGC TCAGCCCAGC
2281CCCCGCGAAT CTTCGTGGCA CACAGCAAGT TTGTCATCCT CAGTGCACAC AAACTGGTGT
2341TCATTGGAGA CACGCTGACA CGGCAGGTGA CTGCCCAGGA CATTCGCAAC AAAGTCATGA
2401ACTCCAGCAA CCAGCTCTGC GAGCAGCTCA AGACCATAGT CATGGCAACC AAGATGGCCG
2461CCCTCCATTA CCCCAGCACC ACGGCCCTGC AGGAAATGGT GCACCAAGTG ACAGACCTTT
2521CTAGAAATGC CCAGCTGTTC AAGCGCTCTT TGCTGGAGAT GGCAACGTTC TAGAACCCAG
2581CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
2641ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
2701TATCTTGTGG AAAGGACGAT CAACGTTTGT TTCGGCCGTG TCAACGCGTT AAGTCgacaa
2761tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)

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