Construct: ORF TRCN0000488609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020356.1_s317c1
- DNA Barcode:
- GACCGGGAGATCTAGTCATGTGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- MAP3K14 (9020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9020 | MAP3K14 | mitogen-activated protein k... | NM_003954.5 | 99.9% | 100% | 2706C>G |
| 2 | human | 9020 | MAP3K14 | mitogen-activated protein k... | XM_011525441.2 | 99.9% | 100% | 2706C>G |
| 3 | human | 9020 | MAP3K14 | mitogen-activated protein k... | XR_934591.1 | 60.8% | (many diffs) | |
| 4 | mouse | 53859 | Map3k14 | mitogen-activated protein k... | NM_016896.3 | 83.6% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2913
- ORF length:
- 2841
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagtg atggaaatgg cctgcccagg tgcccctggc tcagcagtgg 121 ggcagcagaa ggaactcccc aaagccaagg agaagacgcc gccactgggg aagaaacaga 181 gctccgtcta caagcttgag gccgtggaga agagccctgt gttctgcgga aagtgggaga 241 tcctgaatga cgtgattacc aagggcacag ccaaggaagg ctccgaggca gggccagctg 301 ccatctctat catcgcccag gctgagtgtg agaatagcca agagttcagc cccacctttt 361 cagaacgcat tttcatcgct gggtccaaac agtacagcca gtccgagagt cttgatcaga 421 tccccaacaa tgtggcccat gctacagagg gcaaaatggc ccgtgtgtgt tggaagggaa 481 agcgtcgcag caaagcccgg aagaaacgga agaagaagag ctcaaagtcc ctggctcatg 541 caggagtggc cttggccaaa cccctcccca ggacccctga gcaggagagc tgcaccatcc 601 cagtgcagga ggatgagtct ccactcggcg ccccatatgt tagaaacacc ccgcagttca 661 ccaagcctct gaaggaacca ggccttgggc aactctgttt taagcagctt ggcgagggcc 721 tacggccggc tctgcctcga tcagaactcc acaaactgat cagccccttg caatgtctga 781 accacgtgtg gaaactgcac cacccccagg acggaggccc cctgcccctg cccacgcacc 841 ccttccccta tagcagactg cctcatccct tcccattcca ccctctccag ccctggaaac 901 ctcaccctct ggagtccttc ctgggcaaac tggcctgtgt agacagccag aaacccttgc 961 ctgacccaca cctgagcaaa ctggcctgtg tagacagtcc aaagcccctg cctggcccac 1021 acctggagcc cagctgcctg tctcgtggtg cccatgagaa gttttctgtg gaggaatacc 1081 tagtgcatgc tctgcaaggc agcgtgagct caggccaggc ccacagcctg accagcctgg 1141 ccaagacctg ggcagcaagg ggctccagat cccgggagcc cagccccaaa actgaggaca 1201 acgagggtgt cctgctcact gagaaactca agccagtgga ttatgagtac cgagaagaag 1261 tccactgggc cacgcaccag ctccgcctgg gcagaggctc cttcggagag gtgcacagga 1321 tggaggacaa gcagactggc ttccagtgcg ctgtcaaaaa ggtgcggctg gaagtatttc 1381 gggcagagga gctgatggca tgtgcaggat tgacctcacc cagaattgtc cctttgtatg 1441 gagctgtgag agaagggcct tgggtcaaca tcttcatgga gctgctggaa ggtggctccc 1501 tgggccagct ggtcaaggag cagggctgtc tcccagagga ccgggccctg tactacctgg 1561 gccaggccct ggagggtctg gaatacctcc actcacgaag gattctgcat ggggacgtca 1621 aagctgacaa cgtgctcctg tccagcgatg ggagccacgc agccctctgt gactttggcc 1681 atgctgtgtg tcttcaacct gatggcctgg gaaagtcctt gctcacaggg gactacatcc 1741 ctggcacaga gacccacatg gctccggagg tggtgctggg caggagctgc gacgccaagg 1801 tggatgtctg gagcagctgc tgtatgatgc tgcacatgct caacggctgc cacccctgga 1861 ctcagttctt ccgagggccg ctctgcctca agattgccag cgagcctccg cctgtgaggg 1921 agatcccacc ctcctgcgcc cctctcacag cccaggccat ccaagagggg ctgaggaaag 1981 agcccatcca ccgcgtgtct gcagcggagc tgggagggaa ggtgaaccgg gcactacagc 2041 aagtgggagg tctgaagagc ccttggaggg gagaatataa agaaccaaga catccaccgc 2101 caaatcaagc caattaccac cagaccctcc atgcccagcc gagagagctt tcgccaaggg 2161 ccccagggcc ccggccagct gaggagacaa caggcagagc ccctaagctc cagcctcctc 2221 tcccaccaga gcccccagag ccaaacaagt ctcctccctt gactttgagc aaggaggagt 2281 ctgggatgtg ggaaccctta cctctgtcct ccctggagcc agcccctgcc agaaacccca 2341 gctcaccaga gcggaaagca accgtcccgg agcaggaact gcagcagctg gaaatagaat 2401 tattcctcaa cagcctgtcc cagccatttt ctctggagga gcaggagcaa attctctcgt 2461 gcctcagcat cgacagcctc tccctgtcgg atgacagtga gaagaaccca tcaaaggcct 2521 ctcaaagctc gcgggacacc ctgagctcag gcgtacactc ctggagcagc caggccgagg 2581 ctcGAAGCTC CAGCTGGAAC ATGGTGCTGG CCCGGGGGCG GCCCACCGAC ACCCCAAGCT 2641 ATTTCAATGG TGTGAAAGTC CAAATACAGT CTCTTAATGG TGAACACCTG CACATCCGGG 2701 AGTTCCACCG GGTCAAAGTG GGAGACATCG CCACTGGCAT CAGCAGCCAG ATCCCAGCTG 2761 CAGCCTTCAG CTTGGTGACC AAAGACGGGC AGCCTGTTCG CTACGACATG GAGGTGCCAG 2821 ACTCGGGCAT CGACCTGCAG TGCACACTGG CCCCTGATGG CAGCTTCGCC TGGAGCTGGA 2881 GGGTCAAGCA TGGCCAGCTG GAGAACAGGC CCTAGGACCC AGCTTTCTTG TACAAAGTGG 2941 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3001 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3061 AGACCGGGAG ATCTAGTCAT GTGTGACGCG TTAAGTCgac aatcaacctc tggattacaa 3121 aatttgtgaa agatt