Construct: ORF TRCN0000488611
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020166.1_s317c1
- DNA Barcode:
- CGATCCGCAAGACCTGTATATGAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK1 (27102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488611
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27102 | EIF2AK1 | eukaryotic translation init... | NM_014413.4 | 100% | 100% | |
| 2 | human | 27102 | EIF2AK1 | eukaryotic translation init... | NM_001134335.1 | 99.8% | 99.8% | 728_729insAGC |
| 3 | human | 27102 | EIF2AK1 | eukaryotic translation init... | XM_024446718.1 | 66.9% | 66.9% | 0_1ins624 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1962
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcagggg ggcaactccg gggtccgcaa gcgcgaagag gagggcgacg 121 gggctggggc tgtggctgcg ccgccggcca tcgactttcc cgccgagggc ccggaccccg 181 aatatgacga atctgatgtt ccagcagaaa tccaggtgtt aaaagaaccc ctacaacagc 241 caaccttccc ttttgcagtt gcaaaccaac tcttgctggt ttctttgctg gagcacttga 301 gccacgtgca tgaaccaaac ccacttcgtt caagacaggt gtttaagcta ctttgccaga 361 cgtttatcaa aatggggctg ctgtcttctt tcacttgtag tgacgagttt agctcattga 421 gactacatca caacagagct attactcact taatgaggtc tgctaaagag agagttcgtc 481 aggatccttg tgaggatatt tctcgtatcc agaaaatcag atcaagggaa gtagccttgg 541 aagcacaaac ttcacgttac ttaaatgaat ttgaagaact tgccatctta ggaaaaggtg 601 gatacggaag agtatacaag gtcaggaata aattagatgg tcagtattat gcaataaaaa 661 aaatcctgat taagggtgca actaaaacag tttgcatgaa ggtcctacgg gaagtgaagg 721 tgctggcagg tcttcagcac cccaatattg ttggctatca caccgcgtgg atagaacatg 781 ttcatgtgat tcagccacga gcagacagag ctgccattga gttgccatct ctggaagtgc 841 tctccgacca ggaagaggac agagagcaat gtggtgttaa aaatgatgaa agtagcagct 901 catccattat ctttgctgag cccaccccag aaaaagaaaa acgctttgga gaatctgaca 961 ctgaaaatca gaataacaag tcggtgaagt acaccaccaa tttagtcata agagaatctg 1021 gtgaacttga gtcgaccctg gagctccagg aaaatggctt ggctggtttg tctgccagtt 1081 caattgtgga acagcagctg ccactcaggc gtaattccca cctagaggag agtttcacat 1141 ccaccgaaga atcttccgaa gaaaatgtca actttttggg tcagacagag gcacagtacc 1201 acctgatgct gcacatccag atgcagctgt gtgagctctc gctgtgggat tggatagtcg 1261 agagaaacaa gcggggccgg gagtatgtgg acgagtctgc ctgtccttat gttatggcca 1321 atgttgcaac aaaaattttt caagaattgg tagaaggtgt gttttacata cataacatgg 1381 gaattgtgca ccgagatctg aagccaagaa atatttttct tcatggccct gatcagcaag 1441 taaaaatagg agactttggt ctggcctgca cagacatcct acagaagaac acagactgga 1501 ccaacagaaa cgggaagaga acaccaacac atacgtccag agtgggtact tgtctgtacg 1561 cttcacccga acagttggaa ggatctgagt atgatgccaa gtcagatatg tacagcttgg 1621 gtgtggtcct gctagagctc tttcagccgt ttGGAACAGA AATGGAGCGA GCAGAAGTTC 1681 TAACAGGTTT AAGAACTGGT CAGTTGCCGG AATCCCTCCG TAAAAGGTGT CCAGTGCAAG 1741 CCAAGTATAT CCAGCACTTA ACGAGAAGGA ACTCATCGCA GAGACCATCT GCCATTCAGC 1801 TGCTGCAGAG TGAACTTTTC CAAAATTCTG GAAATGTTAA CCTCACCCTA CAGATGAAGA 1861 TAATAGAGCA AGAAAAAGAA ATTGCAGAAC TAAAGAAGCA GCTAAACCTC CTTTCTCAAG 1921 ACAAAGGGGT GAGGGATGAC GGAAAGGATG GGGGCGTGGG ATGAGACCCA GCTTTCTTGT 1981 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2041 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2101 GAAAGGACGA CGATCCGCAA GACCTGTATA TGAGACGCGT TAAGTCgaca atcaacctct 2161 ggattacaaa atttgtgaaa gatt