Construct: ORF TRCN0000488619
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019975.1_s317c1
- DNA Barcode:
- TCCCGTAAGGTTCTACCTGAAGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYSLTR1 (10800)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488619
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282186.1 | 99.6% | 99.7% | 815G>C;927C>T;1010_1011delTA |
| 2 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282187.2 | 99.6% | 99.7% | 815G>C;927C>T;1010_1011delTA |
| 3 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_001282188.2 | 99.6% | 99.7% | 815G>C;927C>T;1010_1011delTA |
| 4 | human | 10800 | CYSLTR1 | cysteinyl leukotriene recep... | NM_006639.4 | 99.6% | 99.7% | 815G>C;927C>T;1010_1011delTA |
| 5 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_001281862.1 | 87% | 86.7% | (many diffs) |
| 6 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_001281859.1 | 83.8% | 83.5% | (many diffs) |
| 7 | mouse | 58861 | Cysltr1 | cysteinyl leukotriene recep... | NM_021476.5 | 83.8% | 83.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1083
- ORF length:
- 1011
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgaa acaggaaatc tgacagtatc ttctgccaca tgccatgaca 121 ctattgatga cttccgcaat caagtgtatt ccaccttgta ctctatgatc tctgttgtag 181 gcttctttgg caatggcttt gtgctctatg tcctcataaa aacctatcac aagaagtcag 241 ccttccaagt atacatgatt aatttagcag tagcagatct actttgtgtg tgcacactgc 301 ctctccgtgt ggtctattat gttcacaaag gcatttggct ctttggtgac ttcttgtgcc 361 gcctcagcac ctatgctttg tatgtcaacc tctattgtag catcttcttt atgacagcca 421 tgagcttttt ccggtgcatt gcaattgttt ttccagtcca gaacattaat ttggttacac 481 agaaaaaagc caggtttgtg tgtgtaggta tttggatttt tgtgattttg accagttctc 541 catttctaat ggccaaacca caaaaagatg agaaaaataa taccaagtgc tttgagcccc 601 cacaagacaa tcaaactaaa aatcatgttt tggtcttgca ttatgtgtca ttgtttgttg 661 gctttatcat cccttttgtt attataattg tctgttacac aatgatcatt ttgaccttac 721 taaaaaaatc aatgaaaaaa aaTCTGTCAA GTCATAAAAA GGCTATAGGA ATGATCATGG 781 TCGTGACCGC TGCCTTTTTA GTCAGTTTCA TGCCATATCA TATTCAACGT ACCATTCACC 841 TTCATTTTTT ACACAATGAA ACTAAACCCT GTGATTCTGT CCTTACAATG CAGAAGTCCG 901 TGGTCATAAC CTTGTCTCTG GCTGCATCCA ATTGTTGCTT TGACCCTCTC CTATATTTCT 961 TTTCTGGGGG TAACTTTAGG AAAAGGCTGT CTACATTTAG AAAGCATTCT TTGTCCAGCG 1021 TGACTTATGT ACCCAGAAAG AAGGCCTCTT TGCCAGAAAA AGGAGAAGAA ATATGTAAAG 1081 TAGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGATC CCGTAAGGTT CTACCTGAAG AGACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt