Construct: ORF TRCN0000488621
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019955.1_s317c1
- DNA Barcode:
- GTTCGACACCAGCTCGGATCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NR1I2 (8856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488621
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8856 | NR1I2 | nuclear receptor subfamily ... | NM_003889.3 | 87% | 86.8% | 1_165del;519_521delGCT;1302_1303insG |
| 2 | human | 8856 | NR1I2 | nuclear receptor subfamily ... | NM_022002.2 | 79.8% | 79.7% | 1_282del;636_638delGCT;1419_1420insG |
| 3 | human | 8856 | NR1I2 | nuclear receptor subfamily ... | NM_033013.2 | 78.9% | 78.8% | 1_165del;518_519ins108;1191_1192insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1209
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgacatgt gaaggatgca agggcttttt caggagggcc atgaaacgca 121 acgcccggct gaggtgcccc ttccggaagg gcgcctgcga gatcacccgg aagacccggc 181 gacagtgcca ggcctgccgc ctgcgcaagt gcctggagag cggcatgaag aaggagatga 241 tcatgtccga cgaggccgtg gaggagaggc gggccttgat caagcggaag aaaagtgaac 301 ggacagggac tcagccactg ggagtgcagg ggctgacaga ggagcagcgg atgatgatca 361 gggagctgat ggacgctcag atgaaaacct ttgacactac cttctcccat ttcaagaatt 421 tccggccagg ggtgcttagc agtggctgcg agttgccaga gtctctgcag gccccatcga 481 gggaagaagc tgccaagtgg agccaggtcc ggaaagatct gtgctctttg aaggtctctc 541 tgcagctgcg gggggaggat ggcagtgtct ggaactacaa acccccagcc gacagtggcg 601 ggaaagagat cttctccctg ctgccccaca tggctgacat gtcaacctac atgttcaaag 661 gcatcatcag ctttgccaaa gtcatctcct acttcaggga cttgcccatc gaggaccaga 721 tctccctgct gaagggggcc gctttcgagc tgtgtcaact gagattcaac acagtgttca 781 acgcggagac tggaacctgg gagtgtggcc ggctgtccta ctgcttggaa gacactgcag 841 gtggcttcca gcaacttcta ctggagccca tgctgaaatt ccactacatg ctgaagaagc 901 tgcagctgca tgaggaggag tatgtgctga tgcaggccat ctcccTCTTC TCCCCAGACC 961 GCCCAGGTGT GCTGCAGCAC CGCGTGGTGG ACCAGCTGCA GGAGCAATTC GCCATTACTC 1021 TGAAGTCCTA CATTGAATGC AATCGGCCCC AGCCTGCTCA TAGGTTCTTG TTCCTGAAGA 1081 TCATGGCTAT GCTCACCGAG CTCCGCAGCA TCAATGCTCA GCACACCCAG CGGCTGCTGC 1141 GCATCCAGGA CATACACCCC TTTGCTACGC CCCTCATGCA GGAGTTGTTC GGCATCACAG 1201 GTAGCGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1261 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1321 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTTCGACAC CAGCTCGGAT CGACCACGCG 1381 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt