Construct: ORF TRCN0000488629
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021629.1_s317c1
- DNA Barcode:
- AGAATCTGATTGATCCGACAGTAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TAB1 (10454)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488629
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10454 | TAB1 | TGF-beta activated kinase 1... | NM_006116.3 | 100% | 100% | |
| 2 | human | 10454 | TAB1 | TGF-beta activated kinase 1... | NM_153497.3 | 88.5% | 85.5% | (many diffs) |
| 3 | mouse | 66513 | Tab1 | TGF-beta activated kinase 1... | NM_025609.2 | 88.3% | 97.2% | (many diffs) |
| 4 | mouse | 66513 | Tab1 | TGF-beta activated kinase 1... | XM_006521259.2 | 58.9% | 65% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1581
- ORF length:
- 1512
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcggcgcag aggaggagct tgctgcagag tgagcagcag ccaagctgga 121 cagatgacct gcctctctgc cacctctctg gggttggctc agcctccaac cgcagctact 181 ctgctgatgg caagggcact gagagccacc cgccagagga cagctggctc aagttcagga 241 gtgagaacaa ctgcttcctg tatggggtct tcaacggcta tgatggcaac cgagtgacca 301 acttcgtggc ccagcggctg tccgcagagc tcctgctggg ccagctgaat gccgagcacg 361 ccgaggccga tgtgcggcgt gtgctgctgc aggccttcga tgtggtggag aggagcttcc 421 tggagtccat tgacgacgcc ttggctgaga aggcaagcct ccagtcgcaa ttgccagagg 481 gagtccctca gcaccagctg cctcctcagt atcagaagat ccttgagaga ctcaagacgt 541 tagagaggga aatttcggga ggggccatgg ccgttgtggc ggtccttctc aacaacaagc 601 tctacgtcgc caatgtcggt acaaaccgtg cacttttatg caaatcgaca gtggatgggt 661 tgcaggtgac acagctgaac gtggaccaca ccacagagaa cgaggatgag ctcttccgtc 721 tttcgcagct gggcttggat gctggaaaga tcaagcaggt ggggatcatc tgtgggcagg 781 agagcacccg gcggatcggg gattacaagg ttaaatatgg ctacacggac attgaccttc 841 tcagcgctgc caagtccaaa ccaatcatcg cagagccaga aatccatggg gcacagccgc 901 tggatggggt gacgggcttc ttggtgctga tgtcggaggg gttgtacaag gccctagagg 961 cagcccatgg gcctgggcag gccaaccagg agattgctgc gatgattgac actgagtttg 1021 ccaagcagac ctccctggac gcagtggccc aggccgtcgt ggaccgggtg aagcgcatcc 1081 acagcgacac cttcgccagt ggtggggagc gtgccaggtt ctgcccccgg cacgaggaca 1141 tgaccctgct agtgaggaac tttggctacc cgctgggcga aatgagccag cccacaccga 1201 gcccagcccc agctgcagga ggacgagtgt accctgtgtc tgtgccatac TCCAGCGCCC 1261 AGAGCACCAG CAAGACCAGC GTGACCCTCT CCCTTGTCAT GCCCTCCCAG GGCCAGATGG 1321 TCAACGGGGC TCACAGTGCT TCCACCCTGG ACGAAGCCAC CCCCACCCTC ACCAACCAAA 1381 GCCCGACCTT AACCCTGCAG TCCACCAACA CGCACACGCA GAGCAGCAGC TCCAGCTCTG 1441 ACGGAGGCCT CTTCCGCTCC CGGCCCGCCC ACTCGCTCCC GCCTGGCGAG GACGGTCGTG 1501 TTGAGCCCTA TGTGGACTTT GCTGAGTTTT ACCGCCTCTG GAGCGTGGAC CATGGCGAGC 1561 AGAGCGTGGT GACAGCACCG TAGAACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1621 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1681 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA GAATCTGATT 1741 GATCCGACAG TACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1801 att