Construct: ORF TRCN0000488643

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020163.1_s317c1
DNA Barcode:
CATCTGATGGTGCTTGCACTGTTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GSK3B (2932)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488643
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2932 GSK3B glycogen synthase kinase 3 ... NM_002093.4 100% 100%
2 human 2932 GSK3B glycogen synthase kinase 3 ... NM_001146156.2 96.9% 96.9% 908_909ins39
3 human 2932 GSK3B glycogen synthase kinase 3 ... XM_006713610.3 92.3% 92.3% 1133_1134ins99
4 human 2932 GSK3B glycogen synthase kinase 3 ... NM_001354596.2 89.3% 89.3% 908_909ins39;1094_1095ins99
5 mouse 56637 Gsk3b glycogen synthase kinase 3 ... NM_001347232.1 92.6% 98.3% (many diffs)
6 mouse 56637 Gsk3b glycogen synthase kinase 3 ... NM_019827.7 89.9% 96% (many diffs)
7 mouse 56637 Gsk3b glycogen synthase kinase 3 ... XM_006522426.4 71.2% 75.2% (many diffs)
8 mouse 56637 Gsk3b glycogen synthase kinase 3 ... XM_030249221.1 68.6% 72.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1371
ORF length:
1299
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgtcaggg cggcccagaa ccacctcctt tgcggagagc tgcaagccgg
121tgcagcagcc ttcagctttt ggcagcatga aagttagcag agacaaggac ggcagcaagg
181tgacaacagt ggtggcaact cctgggcagg gtccagacag gccacaagaa gtcagctata
241cagacactaa agtgattgga aatggatcat ttggtgtggt atatcaagcc aaactttgtg
301attcaggaga actggtcgcc atcaagaaag tattgcagga caagagattt aagaatcgag
361agctccagat catgagaaag ctagatcact gtaacatagt ccgattgcgt tatttcttct
421actccagtgg tgagaagaaa gatgaggtct atcttaatct ggtgctggac tatgttccgg
481aaacagtata cagagttgcc agacactata gtcgagccaa acagacgctc cctgtgattt
541atgtcaagtt gtatatgtat cagctgttcc gaagtttagc ctatatccat tcctttggaa
601tctgccatcg ggatattaaa ccgcagaacc tcttgttgga tcctgatact gctgtattaa
661aactctgtga ctttggaagt gcaaagcagc tggtccgagg agaacccaat gtttcgtata
721tctgttctcg gtactatagg gcaccagagt tgatctttgg agccactgat tatacctcta
781gtatagatgt atggtctgct ggctgtgtgt tggctgagct gttactagga caaccaatat
841ttccagggga tagtggtgtg gatcagttgg tagaaataat caaggtcctg ggaactccaa
901caagggagca aatcagagaa atgaacccaa actacacaga atttaaattc cctcaaatta
961aggcacatcc ttggactaag gattcgtcag gaacaggaca tttcaccTCA GGAGTGCGGG
1021TCTTCCGACC CCGAACTCCA CCGGAGGCAA TTGCACTGTG TAGCCGTCTG CTGGAGTATA
1081CACCAACTGC CCGACTAACA CCACTGGAAG CTTGTGCACA TTCATTTTTT GATGAATTAC
1141GGGACCCAAA TGTCAAACTA CCAAATGGGC GAGACACACC TGCACTCTTC AACTTCACCA
1201CTCAAGAACT GTCAAGTAAT CCACCTCTGG CTACCATCCT TATTCCTCCT CATGCTCGGA
1261TTCAAGCAGC TGCTTCAACC CCCACAAATG CCACAGCAGC GTCAGATGCT AATACTGGAG
1321ACCGTGGACA GACCAATAAT GCTGCTTCTG CATCAGCTTC CAACTCCACC TGAGACCCAG
1381CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1441ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1501TATCTTGTGG AAAGGACGAC ATCTGATGGT GCTTGCACTG TTCACGCGTT AAGTCgacaa
1561tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)