Construct: ORF TRCN0000488643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020163.1_s317c1
- DNA Barcode:
- CATCTGATGGTGCTTGCACTGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GSK3B (2932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2932 | GSK3B | glycogen synthase kinase 3 ... | NM_002093.4 | 100% | 100% | |
| 2 | human | 2932 | GSK3B | glycogen synthase kinase 3 ... | NM_001146156.2 | 96.9% | 96.9% | 908_909ins39 |
| 3 | human | 2932 | GSK3B | glycogen synthase kinase 3 ... | XM_006713610.3 | 92.3% | 92.3% | 1133_1134ins99 |
| 4 | human | 2932 | GSK3B | glycogen synthase kinase 3 ... | NM_001354596.2 | 89.3% | 89.3% | 908_909ins39;1094_1095ins99 |
| 5 | mouse | 56637 | Gsk3b | glycogen synthase kinase 3 ... | NM_001347232.1 | 92.6% | 98.3% | (many diffs) |
| 6 | mouse | 56637 | Gsk3b | glycogen synthase kinase 3 ... | NM_019827.7 | 89.9% | 96% | (many diffs) |
| 7 | mouse | 56637 | Gsk3b | glycogen synthase kinase 3 ... | XM_006522426.4 | 71.2% | 75.2% | (many diffs) |
| 8 | mouse | 56637 | Gsk3b | glycogen synthase kinase 3 ... | XM_030249221.1 | 68.6% | 72.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1371
- ORF length:
- 1299
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcaggg cggcccagaa ccacctcctt tgcggagagc tgcaagccgg 121 tgcagcagcc ttcagctttt ggcagcatga aagttagcag agacaaggac ggcagcaagg 181 tgacaacagt ggtggcaact cctgggcagg gtccagacag gccacaagaa gtcagctata 241 cagacactaa agtgattgga aatggatcat ttggtgtggt atatcaagcc aaactttgtg 301 attcaggaga actggtcgcc atcaagaaag tattgcagga caagagattt aagaatcgag 361 agctccagat catgagaaag ctagatcact gtaacatagt ccgattgcgt tatttcttct 421 actccagtgg tgagaagaaa gatgaggtct atcttaatct ggtgctggac tatgttccgg 481 aaacagtata cagagttgcc agacactata gtcgagccaa acagacgctc cctgtgattt 541 atgtcaagtt gtatatgtat cagctgttcc gaagtttagc ctatatccat tcctttggaa 601 tctgccatcg ggatattaaa ccgcagaacc tcttgttgga tcctgatact gctgtattaa 661 aactctgtga ctttggaagt gcaaagcagc tggtccgagg agaacccaat gtttcgtata 721 tctgttctcg gtactatagg gcaccagagt tgatctttgg agccactgat tatacctcta 781 gtatagatgt atggtctgct ggctgtgtgt tggctgagct gttactagga caaccaatat 841 ttccagggga tagtggtgtg gatcagttgg tagaaataat caaggtcctg ggaactccaa 901 caagggagca aatcagagaa atgaacccaa actacacaga atttaaattc cctcaaatta 961 aggcacatcc ttggactaag gattcgtcag gaacaggaca tttcaccTCA GGAGTGCGGG 1021 TCTTCCGACC CCGAACTCCA CCGGAGGCAA TTGCACTGTG TAGCCGTCTG CTGGAGTATA 1081 CACCAACTGC CCGACTAACA CCACTGGAAG CTTGTGCACA TTCATTTTTT GATGAATTAC 1141 GGGACCCAAA TGTCAAACTA CCAAATGGGC GAGACACACC TGCACTCTTC AACTTCACCA 1201 CTCAAGAACT GTCAAGTAAT CCACCTCTGG CTACCATCCT TATTCCTCCT CATGCTCGGA 1261 TTCAAGCAGC TGCTTCAACC CCCACAAATG CCACAGCAGC GTCAGATGCT AATACTGGAG 1321 ACCGTGGACA GACCAATAAT GCTGCTTCTG CATCAGCTTC CAACTCCACC TGAGACCCAG 1381 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1441 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1501 TATCTTGTGG AAAGGACGAC ATCTGATGGT GCTTGCACTG TTCACGCGTT AAGTCgacaa 1561 tcaacctctg gattacaaaa tttgtgaaag att