Construct: ORF TRCN0000488645
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019295.1_s317c1
- DNA Barcode:
- GTTAGTAATTACTCCAGAGCCAGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ADGRA1 (84435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488645
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | NM_001083909.2 | 43.6% | 7.3% | (many diffs) |
| 2 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | NM_001291085.1 | 36% | 8.5% | 0_1ins2452;207_209delGTA;1389_1390insG |
| 3 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | XM_011540273.1 | 30.5% | 9.7% | 0_1ins2665;1173_1174insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 882
- ORF length:
- 807
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgcga ggccacggga accactcgcg gctgaaatcc agatgtgctt 121 ctctgggcaa cgtccatcac caccaggcac agatggcgca cggtgcacac tgccgagggg 181 gccggacacc acagtcacca gacagccggg gcccaaccgc tgggggccac acccggggca 241 gatggacgta ccggcccatc gaggccctca tcaacgtcag ctttcccgga gcaaagggtg 301 ggagaacggc cgagggcgag ccctcatggg cgtgggtctt cagaagtggc tatttgcaga 361 gacaggctct tcctgggctt cccgtctcca cccgagtggc tgcctgctgg tggcaacacc 421 atccaggcag ggactcaggg ttcttgggac ctggatttat tgacctgctc agagaggagc 481 ccagcggccg tgcagatggg aaggcagctg ctcagaaaac agggcgtctt tatttgttcc 541 cgactttcct aggcaacaaa ttaccagagg ccgccgactc gtttggggaa attgcttctg 601 ggaacagtgt tatttttacc tccgtcttgc aatcctctca cccacctggc accccaccgc 661 ccagcgcacg caagccaagt cccggagctg ccatgctcgt ctgtgaggaa acgctggagc 721 tgggtgccca gggctgctgg gggtccacga gccccccggg gccaggccgt gagcttggtc 781 ggtgggccca cggccggagg ctccaggctg ggaggggctg gctgcaccat gggcctgctc 841 atggccctcc aggccagacg ccccagggac ggcacctggg ctgaccctcg aagatgctcc 901 agggctgtgg agggctcggg cctcaccagg gctgcccttg tgagtccagg gccatcgggg 961 gtggaggagg acaccaggag ggaaggctgc gtgcttgtgg gctcctggag gtcgtccacc 1021 cacagaagcc gtgttctgat tatcaacacg ccctctggca ggaccccctg gagccagggc 1081 tccctcggca gcaccgggcg ctacaaccag acccgggcac caggcagcca gcagcagggg 1141 ctgtgctcgg atgcagctct acacgaggat tttgtgccgg agcctactcc tgccttcttt 1201 caataccctc cacaagcctt cagcaaaacc aaggttcgcg acttggggag gggaggcctt 1261 tatgcctttt ccaaggagga gaaggtatct gaggcccagc gggtagaggc ctacgagacc 1321 cacggccttg gcctgagtac aggccgacag aaccctgtgc cgtccccctg taagcctgga 1381 tgcgtgtgcc agggccttgc gctggacgca gaggcctggc cgaggcaccc tggacgcagg 1441 ccctgtgcgg ccaaggaggc ctgcctgggc ccacctggct gcagcgggga gctgagcctc 1501 cctgaggagg aaggaatcgc acctggggca gcaacagcag ctcctgcctg ggtccctcat 1561 ggccgcgtga gccgcttcct cactgtaaag ctcttcacct atgtaactat gtatcaatgt 1621 aaacaggaac gtatcctccg cctctgcatc tctggtgaat ccgggcgata cgtgcggcgc 1681 tcacgcttcc gcaactttgc agcgctcatg gtgagtacgg gggtcccggg ggtcctgcag 1741 ctgggggcta ggccgctgcg ggggggcggc cacccgtatg gggactccca ggctggaaaa 1801 gttgtggaat ggcgagctgg tgaccgggct tccgggctca gtgccgggga aagaaggttc 1861 ctcagccagt cggagctgca gacctggccc cggacacctc actgtgtagg aaagaaggtt 1921 cctcagccag ttggagctgc agacctagcc ccggacacct cactgtgcag gaaagaaggt 1981 tcctcagcca gtcggagctg cagacctggc cccggacacc tcacccgcag ggaggaagat 2041 tccttcaggc agctggcatt ccccaggttt gaagaggtca ctttcccacc tctcactgct 2101 gcagaaagag caccctctgt ccacggtgga cactctctag ccagccaggg cctcgtcttg 2161 gctggacgga caagaagtgt ggcctcagga gggaagcagg agctctcggg cccgctggct 2221 gcctgcatcc cgacacagga tctgaagaca gtgctctccc tgccccgcta cccaggggag 2281 ttcctgcacc ccgtggtgta cgcgtgcacg gccgtcatgc tgctctgcct cctggcctcc 2341 ttcgtcacct acatcgtgca ccagagcgcc atccgcatca gccgcaaggg ccggcacacg 2401 ctcctgaatt tctgcttcca cgcggccctg accttcactg tgttcgccgg cggcatcaat 2461 cgcaccaagt accccatcct gtgccaggcg gtgggcatcg tgctgcacta ttctacactg 2521 tccaccatgc tgtggatagg agtgaccgcc aggaacatct acaagcaggt gaccaagaag 2581 gcccctctgt gcctggacac agaccagcca ccgtacccca ggcagcccct gctcaggttt 2641 tacctcgtca gcggaggggt cccctttatc atctgtgggg tcacggctgc cacgaacatc 2701 aggaattacg ggacagagga cgaggacacg gcctgctgga tggcctggga gcccagcctg 2761 ggcgccttct acggcccagc cgccatcatc accctggtca cctgtgtgta cttcctgggc 2821 acctacgtgc agctgcggcg ccacccaggg cgcaggtacg agctgcgcac acagcccgag 2881 gagcagcggc ggctggcgac acccgagggc ggccgtggga tccggccagg caccccaccc 2941 gcacacgatg cccccggcgc ctccgtgctg cagaacgagc actcattcca ggcacagctg 3001 cgcgccgccg ccttcacgct gttcctgttc acggccacgt gggccttcgg ggcgctggcg 3061 gtgtcacagg gccacttcct ggacatggtc ttcagctgcc tgtacggcgc cttctgcgtg 3121 accctgggac tcttcgtgct catccaccac tgcgccaagc gtgaggacgt gtggcagtgc 3181 tggtgggcat gctgcccgcc ccgcaaggac gcccaccccg cacttgacgc caacggggcc 3241 gcgctgggcc gcgccgcctg cctgcactcg ccgggactgg gccagccacg gggcttcgcg 3301 cacccaccgg gcccctgcaa gatgaccaac ctgcaggccg cgcagggcca cgccagttgc 3361 ctgtcaccgg ccaccccgtg ctgcgccaag atgcactgcg agccactgac ggcggacgag 3421 gcgcacgtgc acctgcagga ggagggcgcc ttcgggcacg acccccacct gcacgggtgc 3481 cttcagggca gaactaagcc gccctacttt agccggcacc cagcagagga gcccgagtac 3541 gcctaccaca tcccatccag cctggatggc agcccccgca gctcgcgcac agacagcccc 3601 cccagctctc tggatggccc ggcggGGACA CACACGCTGG CCTGCTGCAC CCAGGGCGAC 3661 CCCTTCCCCA TGGTCACCCA GCCCGAGGGC AGTGATGGGA GCCCTGCCCT CTACAGCTGC 3721 CCCACGCAGC CGGGCAGGGA GGCAGCGCTC GGGCCCGGCC ACTTGGAGAT GCTGCGGAGG 3781 ACACAGTCCC TGCCCTTTGG TGGCCCCAGC CAGAACGGGC TGCCCAAGGG TAAATTGCTA 3841 GAAGGCCTGC CGTTTGGCAC CGACGGGACC GGCAACATCC GAACGGGACC CTGGAAAAAC 3901 GAAACTACTG TGGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT 3961 AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT 4021 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT TAGTAATTAC TCCAGAGCCA 4081 GGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt