Construct: ORF TRCN0000488650
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020080.1_s317c1
- DNA Barcode:
- CATCGGACAGCATTTCGAGCAGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KL (9365)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488650
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9365 | KL | klotho | NM_004795.4 | 99.9% | 99.9% | 3036_3037insG |
| 2 | human | 9365 | KL | klotho | XM_006719895.2 | 69.6% | 69.5% | 0_1ins921;2115_2116insG |
| 3 | mouse | 16591 | Kl | klotho | NM_013823.2 | 84.1% | 85.3% | (many diffs) |
| 4 | mouse | 16591 | Kl | klotho | XM_006504917.1 | 46.2% | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3108
- ORF length:
- 3039
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcccgccagc gccccgccgc gccgcccgcg gccgccgccg ccgtcgctgt 121 cgctgctgct ggtgctgctg ggcctgggcg gccgccgcct gcgtgcggag ccgggcgacg 181 gcgcgcagac ctgggcccgt ttctcgcggc ctcctgcccc cgaggccgcg ggcctcttcc 241 agggcacctt ccccgacggc ttcctctggg ccgtgggcag cgccgcctac cagaccgagg 301 gcggctggca gcagcacggc aagggtgcgt ccatctggga tacgttcacc caccaccccc 361 tggcaccccc gggagactcc cggaacgcca gtctgccgtt gggcgccccg tcgccgctgc 421 agcccgccac cggggacgta gccagcgaca gctacaacaa cgtcttccgc gacacggagg 481 cgctgcgcga gctcggggtc actcactacc gcttctccat ctcgtgggcg cgagtgctcc 541 ccaatggcag cgcgggcgtc cccaaccgcg aggggctgcg ctactaccgg cgcctgctgg 601 agcggctgcg ggagctgggc gtgcagcccg tggtcaccct gtaccactgg gacctgcccc 661 agcgcctgca ggacgcctac ggcggctggg ccaaccgcgc cctggccgac cacttcaggg 721 attacgcgga gctctgcttc cgccacttcg gcggtcaggt caagtactgg atcaccatcg 781 acaaccccta cgtggtggcc tggcacggct acgccaccgg gcgcctggcc cccggcatcc 841 ggggcagccc gcggctcggg tacctggtgg cgcacaacct cctcctggct catgccaaag 901 tctggcatct ctacaatact tctttccgtc ccactcaggg aggtcaggtg tccattgccc 961 taagctctca ctggatcaat cctcgaagaa tgaccgacca cagcatcaaa gaatgtcaaa 1021 aatctctgga ctttgtacta ggttggtttg ccaaacccgt atttattgat ggtgactatc 1081 ccgagagcat gaagaataac ctttcatcta ttctgcctga ttttactgaa tctgagaaaa 1141 agttcatcaa aggaactgct gacttttttg ctctttgctt tggacccacc ttgagttttc 1201 aacttttgga ccctcacatg aagttccgcc aattggaatc tcccaacctg aggcaactgc 1261 tttcctggat tgaccttgaa tttaaccatc ctcaaatatt tattgtggaa aatggctggt 1321 ttgtctcagg gaccaccaag agagatgatg ccaaatatat gtattacctc aaaaagttca 1381 tcatggaaac cttaaaagcc atcaagctgg atggggtgga tgtcatcggg tataccgcat 1441 ggtccctcat ggatggtttc gagtggcaca gaggttacag catcaggcgt ggactcttct 1501 atgttgactt tctaagccag gacaagatgt tgttgccaaa gtcttcagcc ttgttctacc 1561 aaaagctgat agagaaaaat ggcttccctc ctttacctga aaatcagccc ctagaaggga 1621 catttccctg tgactttgct tggggagttg ttgacaacta cattcaagta gataccactc 1681 tgtctcagtt taccgacctg aatgtttacc tgtgggatgt ccaccacagt aaaaggctta 1741 ttaaagtgga tggggttgtg accaagaaga ggaaatccta ctgtgttgac tttgctgcca 1801 tccagcccca gatcgcttta ctccaggaaa tgcacgttac acattttcgc ttctccctgg 1861 actgggccct gattctccct ctgggtaacc agtcccaggt gaaccacacc atcctgcagt 1921 actatcgctg catggccagc gagcttgtcc gtgtcaacat caccccagtg gtggccctgt 1981 ggcagcctat ggccccgaac caaggactgc cgcgcctcct ggccaggcag ggcgcctggg 2041 agaaccccta cactgccctg gcctttgcag agtatgcccg actgtgcttt caagagctcg 2101 gccatcacgt caagctttgg ataacgatga atgagccgta tacaaggaat atgacataca 2161 gtgctggcca caaccttctg aaggcccatg ccctggcttg gcatgtgtac aatgaaaagt 2221 ttaggcatgc tcagaatggg aaaatatcca tagccttgca ggctgattgg atagaacctg 2281 cctgcccttt ctcccaaaag gacaaagagg tggctgagag agttttggaa tttgacattg 2341 gctggctggc tgagcccatt ttcggctctg gagattatcc atgggtgatg agggactggc 2401 tgaaccaaag aaacaatttt cttcttcctt atttcactga agatgaaaaa aagctaatcc 2461 agggtacctt tgactttttg gctttaagcc attataccac catccttgta gactcagaaa 2521 aagaagatcc aataaaatac aatgattacc tagaagtgca agaaatgacc gacatcacgt 2581 ggctcaactc ccccagtcag gtggcggtag tgccctgggg gttgcgcaaa gtgctgaact 2641 ggctgaagtt caagtacgga gacctcccca tgtacataat atccaatgga atcgatgacg 2701 ggctgcatgc tgaggacgaC CAGCTGAGGG TGTATTATAT GCAGAATTAC ATAAACGAAG 2761 CTCTCAAAGC CCACATACTG GATGGTATCA ATCTTTGCGG ATACTTTGCT TATTCGTTTA 2821 ACGACCGCAC AGCTCCGAGG TTTGGCCTCT ATCGTTATGC TGCAGATCAG TTTGAGCCCA 2881 AGGCATCCAT GAAACATTAC AGGAAAATTA TTGACAGCAA TGGTTTCCCG GGCCCAGAAA 2941 CTCTGGAAAG ATTTTGTCCA GAAGAATTCA CCGTGTGTAC TGAGTGCAGT TTTTTTCACA 3001 CCCGAAAGTC TTTACTGGCT TTCATAGCTT TTCTATTTTT TGCTTCTATT ATTTCTCTCT 3061 CCCTTATATT TTACTACTCG AAGAAAGGCA GAAGAAGTTA CAAAGACCCA GCTTTCTTGT 3121 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3181 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3241 GAAAGGACGA CATCGGACAG CATTTCGAGC AGGAACGCGT TAAGTCgaca atcaacctct 3301 ggattacaaa atttgtgaaa gatt