Construct: ORF TRCN0000488666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021954.1_s317c1
- DNA Barcode:
- GTTCTATAACAGGGCACCACCCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH2 (4853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488666
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4853 | NOTCH2 | notch receptor 2 | NM_024408.4 | 100% | 100% | |
| 2 | human | 4853 | NOTCH2 | notch receptor 2 | NM_001200001.1 | 49.7% | 49.4% | (many diffs) |
| 3 | human | 101929796 | NOTCH2NLR | notch 2 N-terminal like R | NR_157049.1 | 10.9% | (many diffs) | |
| 4 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364007.2 | 10.6% | 10.1% | (many diffs) |
| 5 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364013.1 | 10.4% | 9.4% | (many diffs) |
| 6 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_006711700.4 | 10.2% | 9.1% | (many diffs) |
| 7 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_011510256.2 | 10.1% | 9.6% | (many diffs) |
| 8 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452030.1 | 10% | 9.5% | (many diffs) |
| 9 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452031.1 | 10% | 9.5% | (many diffs) |
| 10 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452032.1 | 10% | 9.5% | (many diffs) |
| 11 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | XM_024452035.1 | 10% | 9.5% | (many diffs) |
| 12 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_001364006.1 | 9.6% | 9.1% | (many diffs) |
| 13 | human | 100996763 | NOTCH2NLB | notch 2 N-terminal like B | NM_001364008.1 | 9.6% | 9.1% | (many diffs) |
| 14 | human | 100996717 | NOTCH2NLC | notch 2 N-terminal like C | NM_001364012.2 | 9.1% | 8.6% | (many diffs) |
| 15 | human | 388677 | NOTCH2NLA | notch 2 N-terminal like A | NM_203458.4 | 9.1% | 8.6% | (many diffs) |
| 16 | mouse | 18129 | Notch2 | notch 2 | NM_010928.2 | 87% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 7482
- ORF length:
- 7413
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcccgccctg cgccccgctc tgctgtgggc gctgctggcg ctctggctgt 121 gctgcgcggc ccccgcgcat gcattgcagt gtcgagatgg ctatgaaccc tgtgtaaatg 181 aaggaatgtg tgttacctac cacaatggca caggatactg caaatgtcca gaaggcttct 241 tgggggaata ttgtcaacat cgagacccct gtgagaagaa ccgctgccag aatggtggga 301 cttgtgtggc ccaggccatg ctggggaaag ccacgtgccg atgtgcctca gggtttacag 361 gagaggactg ccagtactca acatctcatc catgctttgt gtctcgaccc tgcctgaatg 421 gcggcacatg ccatatgctc agccgggata cctatgagtg cacctgtcaa gtcgggttta 481 caggtaagga gtgccaatgg acggatgcct gcctgtctca tccctgtgca aatggaagta 541 cctgtaccac tgtggccaac cagttctcct gcaaatgcct cacaggcttc acagggcaga 601 aatgtgagac tgatgtcaat gagtgtgaca ttccaggaca ctgccagcat ggtggcacct 661 gcctcaacct gcctggttcc taccagtgcc agtgccctca gggcttcaca ggccagtact 721 gtgacagcct gtatgtgccc tgtgcaccct caccttgtgt caatggaggc acctgtcggc 781 agactggtga cttcactttt gagtgcaact gccttccagg ttttgaaggg agcacctgtg 841 agaggaatat tgatgactgc cctaaccaca ggtgtcagaa tggaggggtt tgtgtggatg 901 gggtcaacac ttacaactgc cgctgtcccc cacaatggac aggacagttc tgcacagagg 961 atgtggatga atgcctgctg cagcccaatg cctgtcaaaa tgggggcacc tgtgccaacc 1021 gcaatggagg ctatggctgt gtatgtgtca acggctggag tggagatgac tgcagtgaga 1081 acattgatga ttgtgccttc gcctcctgta ctccaggctc cacctgcatc gaccgtgtgg 1141 cctccttctc ttgcatgtgc ccagagggga aggcaggtct cctgtgtcat ctggatgatg 1201 catgcatcag caatccttgc cacaaggggg cactgtgtga caccaacccc ctaaatgggc 1261 aatatatttg cacctgccca caaggctaca aaggggctga ctgcacagaa gatgtggatg 1321 aatgtgccat ggccaatagc aatccttgtg agcatgcagg aaaatgtgtg aacacggatg 1381 gcgccttcca ctgtgagtgt ctgaagggtt atgcaggacc tcgttgtgag atggacatca 1441 atgagtgcca ttcagacccc tgccagaatg atgctacctg tctggataag attggaggct 1501 tcacatgtct gtgcatgcca ggtttcaaag gtgtgcattg tgaattagaa ataaatgaat 1561 gtcagagcaa cccttgtgtg aacaatgggc agtgtgtgga taaagtcaat cgtttccagt 1621 gcctgtgtcc tcctggtttc actgggccag tttgccagat tgatattgat gactgttcca 1681 gtactccgtg tctgaatggg gcaaagtgta tcgatcaccc gaatggctat gaatgccagt 1741 gtgccacagg tttcactggt gtgttgtgtg aggagaacat tgacaactgt gaccccgatc 1801 cttgccacca tggtcagtgt caggatggta ttgattccta cacctgcatc tgcaatcccg 1861 ggtacatggg cgccatctgc agtgaccaga ttgatgaatg ttacagcagc ccttgcctga 1921 acgatggtcg ctgcattgac ctggtcaatg gctaccagtg caactgccag ccaggcacgt 1981 caggggttaa ttgtgaaatt aattttgatg actgtgcaag taacccttgt atccatggaa 2041 tctgtatgga tggcattaat cgctacagtt gtgtctgctc accaggattc acagggcaga 2101 gatgtaacat tgacattgat gagtgtgcct ccaatccctg tcgcaagggt gcaacatgta 2161 tcaacggtgt gaatggtttc cgctgtatat gccccgaggg accccatcac cccagctgct 2221 actcacaggt gaacgaatgc ctgagcaatc cctgcatcca tggaaactgt actggaggtc 2281 tcagtggata taagtgtctc tgtgatgcag gctgggttgg catcaactgt gaagtggaca 2341 aaaatgaatg cctttcgaat ccatgccaga atggaggaac ttgtgacaat ctggtgaatg 2401 gatacaggtg tacttgcaag aagggcttta aaggctataa ctgccaggtg aatattgatg 2461 aatgtgcctc aaatccatgc ctgaaccaag gaacctgctt tgatgacata agtggctaca 2521 cttgccactg tgtgctgcca tacacaggca agaattgtca gacagtattg gctccctgtt 2581 ccccaaaccc ttgtgagaat gctgctgttt gcaaagagtc accaaatttt gagagttata 2641 cttgcttgtg tgctcctggc tggcaaggtc agcggtgtac cattgacatt gacgagtgta 2701 tctccaagcc ctgcatgaac catggtctct gccataacac ccagggcagc tacatgtgtg 2761 aatgtccacc aggcttcagt ggtatggact gtgaggagga cattgatgac tgccttgcca 2821 atccttgcca gaatggaggt tcctgtatgg atggagtgaa tactttctcc tgcctctgcc 2881 ttccgggttt cactggggat aagtgccaga cagacatgaa tgagtgtctg agtgaaccct 2941 gtaagaatgg agggacctgc tctgactacg tcaacagtta cacttgcaag tgccaggcag 3001 gatttgatgg agtccattgt gagaacaaca tcaatgagtg cactgagagc tcctgtttca 3061 atggtggcac atgtgttgat gggattaact ccttctcttg cttgtgccct gtgggtttca 3121 ctggatcctt ctgcctccat gagatcaatg aatgcagctc tcatccatgc ctgaatgagg 3181 gaacgtgtgt tgatggcctg ggtacctacc gctgcagctg ccccctgggc tacactggga 3241 aaaactgtca gaccctggtg aatctctgca gtcggtctcc atgtaaaaac aaaggtactt 3301 gcgttcagaa aaaagcagag tcccagtgcc tatgtccatc tggatgggct ggtgcctatt 3361 gtgacgtgcc caatgtctct tgtgacatag cagcctccag gagaggtgtg cttgttgaac 3421 acttgtgcca gcactcaggt gtctgcatca atgctggcaa cacgcattac tgtcagtgcc 3481 ccctgggcta tactgggagc tactgtgagg agcaactcga tgagtgtgcg tccaacccct 3541 gccagcacgg ggcaacatgc agtgacttca ttggtggata cagatgcgag tgtgtcccag 3601 gctatcaggg tgtcaactgt gagtatgaag tggatgagtg ccagaatcag ccctgccaga 3661 atggaggcac ctgtattgac cttgtgaacc atttcaagtg ctcttgccca ccaggcactc 3721 ggggcctact ctgtgaagag aacattgatg actgtgcccg gggtccccat tgccttaatg 3781 gtggtcagtg catggatagg attggaggct acagttgtcg ctgcttgcct ggctttgctg 3841 gggagcgttg tgagggagac atcaacgagt gcctctccaa cccctgcagc tctgagggca 3901 gcctggactg tatacagctc accaatgact acctgtgtgt ttgccgtagt gcctttactg 3961 gccggcactg tgaaaccttc gtcgatgtgt gtccccagat gccctgcctg aatggaggga 4021 cttgtgctgt ggccagtaac atgcctgatg gtttcatttg ccgttgtccc ccgggatttt 4081 ccggggcaag gtgccagagc agctgtggac aagtgaaatg taggaagggg gagcagtgtg 4141 tgcacaccgc ctctggaccc cgctgcttct gccccagtcc ccgggactgc gagtcaggct 4201 gtgccagtag cccctgccag cacgggggca gctgccaccc tcagcgccag cctccttatt 4261 actcctgcca gtgtgcccca ccattctcgg gtagccgctg tgaactctac acggcacccc 4321 ccagcacccc tcctgccacc tgtctgagcc agtattgtgc cgacaaagct cgggatggcg 4381 tctgtgatga ggcctgcaac agccatgcct gccagtggga tgggggtgac tgttctctca 4441 ccatggagaa cccctgggcc aactgctcct ccccacttcc ctgctgggat tatatcaaca 4501 accagtgtga tgagctgtgc aacacggtcg agtgcctgtt tgacaacttt gaatgccagg 4561 ggaacagcaa gacatgcaag tatgacaaat actgtgcaga ccacttcaaa gacaaccact 4621 gtgaccaggg gtgcaacagt gaggagtgtg gttgggatgg gctggactgt gctgctgacc 4681 aacctgagaa cctggcagaa ggtaccctgg ttattgtggt attgatgcca cctgaacaac 4741 tgctccagga tgctcgcagc ttcttgcggg cactgggtac cctgctccac accaacctgc 4801 gcattaagcg ggactcccag ggggaactca tggtgtaccc ctattatggt gagaagtcag 4861 ctgctatgaa gaaacagagg atgacacgca gatcccttcc tggtgaacaa gaacaggagg 4921 tggctggctc taaagtcttt ctggaaattg acaaccgcca gtgtgttcaa gactcagacc 4981 actgcttcaa gaacacggat gcagcagcag ctctcctggc ctctcacgcc atacagggga 5041 ccctgtcata ccctcttgtg tctgtcgtca gtgaatccct gactccagaa cgcactcagc 5101 tcctctatct ccttgctgtt gctgttgtca tcattctgtt tattattctg ctgggggtaa 5161 tcatggcaaa acgaaagcgt aagcatggct ctctctggct gcctgaaggt ttcactcttc 5221 gccgagatgc aagcaatcac aagcgtcgtg agccagtggg acaggatgct gtggggctga 5281 aaaatctctc agtgcaagtc tcagaagcta acctaattgg tactggaaca agtgaacact 5341 gggtcgatga tgaagggccc cagccaaaga aagtaaaggc tgaagatgag gccttactct 5401 cagaagaaga tgaccccatt gatcgacggc catggacaca gcagcacctt gaagctgcag 5461 acatccgtag gacaccatcg ctggctctca cccctcctca ggcagagcag gaggtggatg 5521 tgttagatgt gaatgtccgt ggcccagatg gctgcacccc attgatgttg gcttctctcc 5581 gaggaggcag ctcagatttg agtgatgaag atgaagatgc agaggactct tctgctaaca 5641 tcatcacaga cttggtctac cagggtgcca gcctccaggc ccagacagac cggactggtg 5701 agatggccct gcaccttgca gcccgctact cacgggctga tgctgccaag cgtctcctgg 5761 atgcaggtgc agatgccaat gcccaggaca acatgggccg ctgtccactc catgctgcag 5821 tggcagctga tgcccaaggt gtcttccaga ttctgattcg caaccgagta actgatctag 5881 atgccaggat gaatgatggt actacacccc tgatcctggc tgcccgcctg gctgtggagg 5941 gaatggtggc agaactgatc aactgccaag cggatgtgaa tgcagtggat gaccatggaa 6001 aatctgctct tcactgggca gctgctgtca ataatgtgga ggcaactctt ttgttgttga 6061 aaaatggggc caaccgagac atgcaggaca acaaggaaga gacacctctg tttcttgctg 6121 cccgggaggg gagctatgaa gcagccaaga tcctgttaga ccattttgcc aatcgagaca 6181 tcacagacca tatggatcgt cttccccggg atgtggctcg ggatcgcatg caccatgaca 6241 ttgtgcgcct tctggatgaa tacaatgtga ccccaagccc tccaggcacc gtgttgactt 6301 ctgctctctc acctgtcatc tgtgggccca acagatcttt cctcagcctg aagcacaccc 6361 caatgggcaa gaagtctaga cggcccagtg ccaagagtac catgcctact agcctcccta 6421 accttgccaa ggaggcaaag gatgccaagg gtagtaggag gaagaagtct ctgagtgaga 6481 aggtccaact gtctgagagt tcagtaactt tatcccctgt tgattcccta gaatctcctc 6541 acacgtatgt ttccgacacc acatcctctc caatgattac atcccctggg atcttacagg 6601 cctcacccaa ccctatgttg gccactgccg cccctcctgc cccagtccat gcccagcatg 6661 cactatcttt ttctaacctt catgaaatgc agcctttggc acatggggcc agcactgtgc 6721 ttccctcagt gagccagttg ctatcccacc accacattgt gtctccaggc agtggcagtg 6781 ctggaagctt gagtaggctc catccagtcc cagtcccagc agattggatg aaccgcatgg 6841 aggtgaatga gacccagtac aatgagatgt ttggtatggt cctggctcca gctgagggca 6901 cccatcctgg catagctccc cagagcaggc cacctgaagg gaagcacata accacccctc 6961 gggagccctt gccccccatt gtgactttcc agctcatccc taaaggcagt attgcccaac 7021 cagcgggggc tccccagcct cagtccacct gccctccagc tgttgcgggc cccctgccca 7081 ccatgtacca gattccagaa atggcccgtt tgcccagtgt ggctttcccc actgccatga 7141 tgccccagca ggacggGCAG GTAGCTCAGA CCATTCTCCC AGCCTATCAT CCTTTCCCAG 7201 CCTCTGTGGG CAAGTACCCC ACACCCCCTT CACAGCACAG TTATGCTTCC TCAAATGCTG 7261 CTGAGCGAAC ACCCAGTCAC AGTGGTCACC TCCAGGGTGA GCATCCCTAC CTGACACCAT 7321 CCCCAGAGTC TCCTGACCAG TGGTCAAGTT CATCACCCCA CTCTGCTTCT GACTGGTCAG 7381 ATGTGACCAC CAGCCCTACC CCTGGGGGTG CTGGAGGAGG TCAGCGGGGA CCTGGGACAC 7441 ACATGTCTGA GCCACCACAC AACAACATGC AGGTTTATGC GTGAGACCCA GCTTTCTTGT 7501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 7561 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 7621 GAAAGGACGA GTTCTATAAC AGGGCACCAC CCTGACGCGT TAAGTCgaca atcaacctct 7681 ggattacaaa atttgtgaaa gatt