Construct: ORF TRCN0000488667
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021739.1_s317c1
- DNA Barcode:
- GGGTCATTCGTTCACAAAACCAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- NOTCH1 (4851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488667
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4851 | NOTCH1 | notch receptor 1 | NM_017617.5 | 99.9% | 99.9% | 2585_2586insAGC;6555C>T;6648G>A |
| 2 | human | 4851 | NOTCH1 | notch receptor 1 | XM_011518717.2 | 90.4% | 90.3% | (many diffs) |
| 3 | mouse | 18128 | Notch1 | notch 1 | NM_008714.3 | 85.2% | 90.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 7737
- ORF length:
- 7668
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gccgccgctc ctggcgcccc tgctctgcct ggcgctgctg cccgcgctcg 121 ccgcacgagg cccgcgatgc tcccagcccg gtgagacctg cctgaatggc gggaagtgtg 181 aagcggccaa tggcacggag gcctgcgtct gtggcggggc cttcgtgggc ccgcgatgcc 241 aggaccccaa cccgtgcctc agcaccccct gcaagaacgc cgggacatgc cacgtggtgg 301 accgcagagg cgtggcagac tatgcctgca gctgtgccct gggcttctct gggcccctct 361 gcctgacacc cctggacaat gcctgcctca ccaacccctg ccgcaacggg ggcacctgcg 421 acctgctcac gctgacggag tacaagtgcc gctgcccgcc cggctggtca gggaaatcgt 481 gccagcaggc tgacccgtgc gcctccaacc cctgcgccaa cggtggccag tgcctgccct 541 tcgaggcctc ctacatctgc cactgcccac ccagcttcca tggccccacc tgccggcagg 601 atgtcaacga gtgtggccag aagcccgggc tttgccgcca cggaggcacc tgccacaacg 661 aggtcggctc ctaccgctgc gtctgccgcg ccacccacac tggccccaac tgcgagcggc 721 cctacgtgcc ctgcagcccc tcgccctgcc agaacggggg cacctgccgc cccacgggcg 781 acgtcaccca cgagtgtgcc tgcctgccag gcttcaccgg ccagaactgt gaggaaaata 841 tcgacgattg tccaggaaac aactgcaaga acgggggtgc ctgtgtggac ggcgtgaaca 901 cctacaactg ccgctgcccg ccagagtgga caggtcagta ctgtaccgag gatgtggacg 961 agtgccagct gatgccaaat gcctgccaga acggcgggac ctgccacaac acccacggtg 1021 gctacaactg cgtgtgtgtc aacggctgga ctggtgagga ctgcagcgag aacattgatg 1081 actgtgccag cgccgcctgc ttccacggcg ccacctgcca tgaccgtgtg gcctccttct 1141 actgcgagtg tccccatggc cgcacaggtc tgctgtgcca cctcaacgac gcatgcatca 1201 gcaacccctg taacgagggc tccaactgcg acaccaaccc tgtcaatggc aaggccatct 1261 gcacctgccc ctcggggtac acgggcccgg cctgcagcca ggacgtggat gagtgctcgc 1321 tgggtgccaa cccctgcgag catgcgggca agtgcatcaa cacgctgggc tccttcgagt 1381 gccagtgtct gcagggctac acgggccccc gatgcgagat cgacgtcaac gagtgcgtct 1441 cgaacccgtg ccagaacgac gccacctgcc tggaccagat tggggagttc cagtgcatct 1501 gcatgcccgg ctacgagggt gtgcactgcg aggtcaacac agacgagtgt gccagcagcc 1561 cctgcctgca caatggccgc tgcctggaca agatcaatga gttccagtgc gagtgcccca 1621 cgggcttcac tgggcatctg tgccagtacg atgtggacga gtgtgccagc accccctgca 1681 agaatggtgc caagtgcctg gacggaccca acacttacac ctgtgtgtgc acggaagggt 1741 acacggggac gcactgcgag gtggacatcg atgagtgcga ccccgacccc tgccactacg 1801 gctcctgcaa ggacggcgtc gccaccttca cctgcctctg ccgcccaggc tacacgggcc 1861 accactgcga gaccaacatc aacgagtgct ccagccagcc ctgccgccac gggggcacct 1921 gccaggaccg cgacaacgcc tacctctgct tctgcctgaa ggggaccaca ggacccaact 1981 gcgagatcaa cctggatgac tgtgccagca gcccctgcga ctcgggcacc tgtctggaca 2041 agatcgatgg ctacgagtgt gcctgtgagc cgggctacac agggagcatg tgtaacatca 2101 acatcgatga gtgtgcgggc aacccctgcc acaacggggg cacctgcgag gacggcatca 2161 atggcttcac ctgccgctgc cccgagggct accacgaccc cacctgcctg tctgaggtca 2221 atgagtgcaa cagcaacccc tgcgtccacg gggcctgccg ggacagcctc aacgggtaca 2281 agtgcgactg tgaccctggg tggagtggga ccaactgtga catcaacaac aatgagtgtg 2341 aatccaaccc ttgtgtcaac ggcggcacct gcaaagacat gaccagtggc tacgtgtgca 2401 cctgccggga gggcttcagc ggtcccaact gccagaccaa catcaacgag tgtgcgtcca 2461 acccatgtct gaaccagggc acgtgtattg acgacgttgc cgggtacaag tgcaactgcc 2521 tgctgcccta cacaggtgcc acgtgtgagg tggtgctggc cccgtgtgcc cccagcccct 2581 gcagaaacgg cggggagtgc aggcaatccg aggactatga gagcttctcc tgtgtctgcc 2641 ccacgggctg gcaagcaggg cagacctgtg aggtcgacat caacgagtgc gttctgagcc 2701 cgtgccggca cggcgcatcc tgccagaaca cccacggcgg ctaccgctgc cactgccagg 2761 ccggctacag tgggcgcaac tgcgagaccg acatcgacga ctgccggccc aacccgtgtc 2821 acaacggggg ctcctgcaca gacggcatca acacggcctt ctgcgactgc ctgcccggct 2881 tccggggcac tttctgtgag gaggacatca acgagtgtgc cagtgacccc tgccgcaacg 2941 gggccaactg cacggactgc gtggacagct acacgtgcac ctgccccgca ggcttcagcg 3001 ggatccactg tgagaacaac acgcctgact gcacagagag ctcctgcttc aacggtggca 3061 cctgcgtgga cggcatcaac tcgttcacct gcctgtgtcc acccggcttc acgggcagct 3121 actgccagca cgatgtcaat gagtgcgact cacagccctg cctgcatggc ggcacctgtc 3181 aggacggctg cggctcctac aggtgcacct gcccccaggg ctacactggc cccaactgcc 3241 agaaccttgt gcactggtgt gactcctcgc cctgcaagaa cggcggcaaa tgctggcaga 3301 cccacaccca gtaccgctgc gagtgcccca gcggctggac cggcctttac tgcgacgtgc 3361 ccagcgtgtc ctgtgaggtg gctgcgcagc gacaaggtgt tgacgttgcc cgcctgtgcc 3421 agcatggagg gctctgtgtg gacgcgggca acacgcacca ctgccgctgc caggcgggct 3481 acacaggcag ctactgtgag gacctggtgg acgagtgctc acccagcccc tgccagaacg 3541 gggccacctg cacggactac ctgggcggct actcctgcaa gtgcgtggcc ggctaccacg 3601 gggtgaactg ctctgaggag atcgacgagt gcctctccca cccctgccag aacgggggca 3661 cctgcctcga cctccccaac acctacaagt gctcctgccc acggggcact cagggtgtgc 3721 actgtgagat caacgtggac gactgcaatc cccccgttga ccccgtgtcc cggagcccca 3781 agtgctttaa caacggcacc tgcgtggacc aggtgggcgg ctacagctgc acctgcccgc 3841 cgggcttcgt gggtgagcgc tgtgaggggg atgtcaacga gtgcctgtcc aatccctgcg 3901 acgcccgtgg cacccagaac tgcgtgcagc gcgtcaatga cttccactgc gagtgccgtg 3961 ctggtcacac cgggcgccgc tgcgagtccg tcatcaatgg ctgcaaaggc aagccctgca 4021 agaatggggg cacctgcgcc gtggcctcca acaccgcccg cgggttcatc tgcaagtgcc 4081 ctgcgggctt cgagggcgcc acgtgtgaga atgacgctcg tacctgcggc agcctgcgct 4141 gcctcaacgg cggcacatgc atctccggcc cgcgcagccc cacctgcctg tgcctgggcc 4201 ccttcacggg ccccgaatgc cagttcccgg ccagcagccc ctgcctgggc ggcaacccct 4261 gctacaacca ggggacctgt gagcccacat ccgagagccc cttctaccgt tgcctgtgcc 4321 ccgccaaatt caacgggctc ttgtgccaca tcctggacta cagcttcggg ggtggggccg 4381 ggcgcgacat ccccccgccg ctgatcgagg aggcgtgcga gctgcccgag tgccaggagg 4441 acgcgggcaa caaggtctgc agcctgcagt gcaacaacca cgcgtgcggc tgggacggcg 4501 gtgactgctc cctcaacttc aatgacccct ggaagaactg cacgcagtct ctgcagtgct 4561 ggaagtactt cagtgacggc cactgtgaca gccagtgcaa ctcagccggc tgcctcttcg 4621 acggctttga ctgccagcgt gcggaaggcc agtgcaaccc cctgtacgac cagtactgca 4681 aggaccactt cagcgacggg cactgcgacc agggctgcaa cagcgcggag tgcgagtggg 4741 acgggctgga ctgtgcggag catgtacccg agaggctggc ggccggcacg ctggtggtgg 4801 tggtgctgat gccgccggag cagctgcgca acagctcctt ccacttcctg cgggagctca 4861 gccgcgtgct gcacaccaac gtggtcttca agcgtgacgc acacggccag cagatgatct 4921 tcccctacta cggccgcgag gaggagctgc gcaagcaccc catcaagcgt gccgccgagg 4981 gctgggccgc acctgacgcc ctgctgggcc aggtgaaggc ctcgctgctc cctggtggca 5041 gcgagggtgg gcggcggcgg agggagctgg accccatgga cgtccgcggc tccatcgtct 5101 acctggagat tgacaaccgg cagtgtgtgc aggcctcctc gcagtgcttc cagagtgcca 5161 ccgacgtggc cgcattcctg ggagcgctcg cctcgctggg cagcctcaac atcccctaca 5221 agatcgaggc cgtgcagagt gagaccgtgg agccgccccc gccggcgcag ctgcacttca 5281 tgtacgtggc ggcggccgcc tttgtgcttc tgttcttcgt gggctgcggg gtgctgctgt 5341 cccgcaagcg ccggcggcag catggccagc tctggttccc tgagggcttc aaagtgtctg 5401 aggccagcaa gaagaagcgg cgggagcccc tcggcgagga ctccgtgggc ctcaagcccc 5461 tgaagaacgc ttcagacggt gccctcatgg acgacaacca gaatgagtgg ggggacgagg 5521 acctggagac caagaagttc cggttcgagg agcccgtggt tctgcctgac ctggacgacc 5581 agacagacca ccggcagtgg actcagcagc acctggatgc cgctgacctg cgcatgtctg 5641 ccatggcccc cacaccgccc cagggtgagg ttgacgccga ctgcatggac gtcaatgtcc 5701 gcgggcctga tggcttcacc ccgctcatga tcgcctcctg cagcgggggc ggcctggaga 5761 cgggcaacag cgaggaagag gaggacgcgc cggccgtcat ctccgacttc atctaccagg 5821 gcgccagcct gcacaaccag acagaccgca cgggcgagac cgccttgcac ctggccgccc 5881 gctactcacg ctctgatgcc gccaagcgcc tgctggaggc cagcgcagat gccaacatcc 5941 aggacaacat gggccgcacc ccgctgcatg cggctgtgtc tgccgacgca caaggtgtct 6001 tccagatcct gatccggaac cgagccacag acctggatgc ccgcatgcat gatggcacga 6061 cgccactgat cctggctgcc cgcctggccg tggagggcat gctggaggac ctcatcaact 6121 cacacgccga cgtcaacgcc gtagatgacc tgggcaagtc cgccctgcac tgggccgccg 6181 ccgtgaacaa tgtggatgcc gcagttgtgc tcctgaagaa cggggctaac aaagatatgc 6241 agaacaacag ggaggagaca cccctgtttc tggccgcccg ggagggcagc tacgagaccg 6301 ccaaggtgct gctggaccac tttgccaacc gggacatcac ggatcatatg gaccgcctgc 6361 cgcgcgacat cgcacaggag cgcatgcatc acgacatcgt gaggctgctg gacgagtaca 6421 acctggtgcg cagcccgcag ctgcacggag ccccgctggg gggcacgccc accctgtcgc 6481 ccccgctctg ctcgcccaac ggctacctgg gcagcctcaa gcccggcgtg cagggcaaga 6541 aggtccgcaa gcccagcagc aaaggcctgg cctgtggaag caaggaggcc aaggacctca 6601 aggcacggag gaagaagtcc caggatggca agggctgcct gctggacagc tccggcatgc 6661 tctcgcccgt ggactccctg gagtcacccc atggctacct gtcagacgtg gcctcgccac 6721 cactgctgcc ctccccgttc cagcagtctc cgtccgtgcc cctcaaccac ctgcctggga 6781 tgcccgacac ccacctgggc atcgggcacc tgaacgtggc ggccaagccc gagatggcgg 6841 cgctgggtgg gggcggccgg ctggcctttg agactggccc acctcgtctc tcccacctgc 6901 ctgtggcctc tggcaccagc accgtcctgg gctccagcag cggaggggcc ctgaatttca 6961 ctgtgggcgg gtccaccagt ttgaatggtc aatgcgagtg gctgtcccgg ctgcagagcg 7021 gcatggtgcc gaaccaatac aaccctctgc gggggagtgt ggcaccaggc cccctgagca 7081 cacaggcccc ctccctgcag catggcatgg taggcccgct gcacagtagc cttgctgcca 7141 gcgccctgtc ccagatgatg agctaccagg gcctgcccag cacccggctg gccacccagc 7201 ctcacctggt gcagacccag caggtgcagc cacaaaactt acagatgcag cagcagaacc 7261 tgcagccagc aaacatccag cagcagcaaa gcctgcagcc gccaccacca ccaccacagc 7321 cgcaccttgg cgtgagctca gcagccagcg gccacctggg ccggagcttc ctgagtggag 7381 agccgagcca ggcagacgtg cagccactgg gccccagcag cctggcggtg cacactattc 7441 tgccCCAGGA GAGCCCCGCC CTGCCCACGT CGCTGCCATC CTCGCTGGTC CCACCCGTGA 7501 CCGCAGCCCA GTTCCTGACG CCCCCCTCGC AGCACAGCTA CTCCTCGCCT GTGGACAACA 7561 CCCCCAGCCA CCAGCTACAG GTGCCTGAGC ACCCCTTCCT CACCCCGTCC CCTGAGTCCC 7621 CTGACCAGTG GTCCAGCTCG TCCCCGCATT CCAACGTCTC CGACTGGTCC GAGGGCGTCT 7681 CCAGCCCTCC CACCAGCATG CAGTCCCAGA TCGCCCGCAT TCCGGAGGCC TTCAAGTAAG 7741 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 7801 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 7861 TTTATATATC TTGTGGAAAG GACGAGGGTC ATTCGTTCAC AAAACCAAGA CGCGTTAAGT 7921 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt