Construct: ORF TRCN0000488674
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021202.1_s317c1
- DNA Barcode:
- ACGCCATCCGCCAAGCCGAGTGTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPR158 (57512)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488674
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57512 | GPR158 | G protein-coupled receptor 158 | NM_020752.3 | 76.3% | 100% | (many diffs) |
| 2 | human | 57512 | GPR158 | G protein-coupled receptor 158 | XM_017016452.2 | 43.3% | 57.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3717
- ORF length:
- 3645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcgcc atggcctacc ccctgctgct gtgtctgctg ctggcccagc 121 tcggactggg agccgtgggc gccagcagag atcctcaggg cagacccgac agccccagag 181 agagaacccc caagggcaag ccccacgccc agcagcctgg cagagccagc gccagcgata 241 gcagcgcccc ctggtccaga agcaccgacg gcacaatcct ggcccagaaa ctggccgaag 301 aggtgcccat ggacgtggcc agctacctgt acaccggcga cagccaccag ctgaagcggg 361 ccaactgcag cggcagatac gagctggccg gcctgcctgg aaagtggcct gccctggcca 421 gcgcccaccc tagtctgcac agagccctgg acaccctgac ccacgccacc aactttctga 481 acgtgatgct gcagagcaac aagagcagag agcagaacct gcaggacgac ctggactggt 541 atcaggccct cgtgtggtcc ctgctggaag gcgagcccag catcagcaga gccgccatca 601 ccttcagcac cgacagcctg agcgcccctg cccctcaggt gttcctgcag gccacccggg 661 aagagagccg gattctcctc caggatctct cttcctccgc ccctcacctg gccaatgcca 721 ccctggaaac cgagtggttc cacggcctgc ggcggaagtg gcggccacat ctgcacagaa 781 gaggacccaa ccagggccct agaggcctgg gacacagctg gcggagaaag gatggcctgg 841 gcggcgacaa gagccacttc aagtggtccc ccccctacct ggaatgcgag aacggcagct 901 acaagcccgg ctggctggtc acactgagca gcgccatcta cggcctgcag cccaacctgg 961 tgcccgagtt ccggggcgtg atgaaggtgg acatcaacct gcagaaagtg gacatcgacc 1021 agtgcagcag cgacggctgg ttcagcggca cccacaagtg ccacctgaac aacagcgagt 1081 gcatgcccat caagggcctg ggcttcgtgc tgggcgccta cgagtgcatc tgcaaggccg 1141 gcttctacca ccccggcgtg ctgcccgtga acaacttcag acgcagaggc cccgatcagc 1201 acatcagcgg cagcaccaag gacgtgtccg aagaggccta cgtgtgcctg ccctgcagag 1261 agggctgccc cttctgcgcc gacgacagcc catgcttcgt gcaggaagat aagtacctgc 1321 ggctggccat catcagtttt caggccctgt gcatgctgct ggacttcgtg tccatgctgg 1381 tggtgtacca cttccggaag gccaagagca tccgggccag cggcctgatc ctgctggaaa 1441 ccatcctgtt cggctcactg ctcctgtact tccccgtggt catcctgtac ttcgagccct 1501 ccaccttccg gtgcatcctg ctgagatggg ccagactgct gggcttcgcc accgtgtacg 1561 gcaccgtgac cctgaagctg caccgggtgc tgaaagtgtt cctgagccgg accgcccagc 1621 ggatccccta catgacaggc ggcagagtga tgcggatgct ggctgtgatc ctcctggtcg 1681 tgttctggtt cctgatcggc tggaccagca gcgtgtgcca gaacctggaa aagcagatca 1741 gcctgatcgg ccagggcaag accagcgacc acctgatctt caacatgtgc ctgatcgacc 1801 gctgggacta catgaccgcc gtggccgagt tcctgttcct gctgtggggc gtgtacctgt 1861 gctacgccgt gcggacagtg cccagcgcct ttcacgagcc ccggtatatg gccgtggccg 1921 tgcacaacga gctgatcatc tccgccatct tccacaccat cagattcgtg ctcgcttcta 1981 gactgcagag cgactggatg ctgatgctgt actttgccca cacccacctg accgtgaccg 2041 tgacaatcgg cctgctgctg atccccaagt tcagccacag cagcaacaac ccccgggacg 2101 atatcgccac cgaggcctac gaggacgagc tggacatggg ccgcagcggc agctacctga 2161 acagcagcat caacagcgct tggagcgagc acagcctgga ccccgaggac atccgggacg 2221 agctgaagaa gctgtacgcc cagctggaaa tctacaagcg gaagaagatg atcaccaaca 2281 accctcacct ccagaagaag cgctgcagca agaagggact gggccggtcc atcatgcggc 2341 ggatcaccga gatccccgag acagtgtccc ggcagtgcag caaagaggac aaagagggcg 2401 ccgaccacgg caccgccaag ggaacagccc tgatccggaa gaaccccccc gagagcagcg 2461 gcaacaccgg caagtccaaa gaggaaaccc tgaagaaccg ggtgttcagc ctgaagaagt 2521 cccacagcac ctacgaccac gtgcgggacc agaccgagga aagcagcagc ctgcccaccg 2581 agagccagga agaggaaaca accgagaaca gcaccctgga atccctgagc ggcaagaagc 2641 tgacccagaa gctgaaagag gacagcgagg ccgagagcac cgagagcgtg cccctcgtgt 2701 gcaagagcgc ctccgcccac aacctgtcca gcgagaagaa aaccggccac ccccggacca 2761 gcatgctgca gaaaagcctg agcgtgatcg ccagcgccaa agaaaagacc ctcggcctgg 2821 ctggcaagac tcagacagcc ggcgtggaag agaggaccaa gagccagaag cccctgccca 2881 aggacaaaga aacaaaccgg aaccacagca atagcgacaa caccgagaca aaggaccctg 2941 ccccccagaa cagcaacccc gccgaggaac cccggaagcc ccagaaaagc ggcatcatga 3001 agcagcagcg cgtgaacccc accaccgcca acagcgacct gaaccctggc accacccaga 3061 tgaaggacaa cttcgacatc ggcgaagtgt gcccctggga ggtgtacgac ctgaccccag 3121 gccccgtgcc cagcgagagc aaggtgcaga aacacgtgtc catcgtggcc agcgagatgg 3181 aaaagaaccc cacctttagc ctgaaagaga agtctcacca caagcccaag gccgctgaag 3241 tgtgccagca gagcaaccag aagcggatcg acaaggccga agtctgcctg tgggagagcc 3301 agggccagag catcctggaa gatgagaagc tgctgattag caagaccccc gtgctgcctg 3361 agcgggccaa agaagagaac ggcggccagc ccagggccgc caatgtctgt gccggccaga 3421 gcgaggaact gccccctaaa gccgtggcca gcaagaccga gaacgagaac cTGAACCAGA 3481 TCGGCCACCA GGAAAAGAAA ACCAGCAGCA GCGAGGAAAA CGTGCGGGGC TCCTACAACA 3541 GCTCCAACAA CTTCCAGCAG CCCCTGACCA GCAGGGCTGA AGTCTGTCCA TGGGAATTCG 3601 AGACACCCGC CCAGCCCAAC GCCGGCAGAT CTGTGGCCCT GCCTGCCAGC TCTGCCCTGA 3661 GCGCCAACAA GATCGCCGGA CCCCGGAAAG AGGAAATCTG GGACAGCTTC AAAGTGTGAG 3721 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3841 TTTATATATC TTGTGGAAAG GACGAACGCC ATCCGCCAAG CCGAGTGTGA CGCGTTAAGT 3901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt