Construct: ORF TRCN0000488674

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021202.1_s317c1
DNA Barcode:
ACGCCATCCGCCAAGCCGAGTGTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GPR158 (57512)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488674
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57512 GPR158 G protein-coupled receptor 158 NM_020752.3 76.3% 100% (many diffs)
2 human 57512 GPR158 G protein-coupled receptor 158 XM_017016452.2 43.3% 57.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3717
ORF length:
3645
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgggcgcc atggcctacc ccctgctgct gtgtctgctg ctggcccagc
121tcggactggg agccgtgggc gccagcagag atcctcaggg cagacccgac agccccagag
181agagaacccc caagggcaag ccccacgccc agcagcctgg cagagccagc gccagcgata
241gcagcgcccc ctggtccaga agcaccgacg gcacaatcct ggcccagaaa ctggccgaag
301aggtgcccat ggacgtggcc agctacctgt acaccggcga cagccaccag ctgaagcggg
361ccaactgcag cggcagatac gagctggccg gcctgcctgg aaagtggcct gccctggcca
421gcgcccaccc tagtctgcac agagccctgg acaccctgac ccacgccacc aactttctga
481acgtgatgct gcagagcaac aagagcagag agcagaacct gcaggacgac ctggactggt
541atcaggccct cgtgtggtcc ctgctggaag gcgagcccag catcagcaga gccgccatca
601ccttcagcac cgacagcctg agcgcccctg cccctcaggt gttcctgcag gccacccggg
661aagagagccg gattctcctc caggatctct cttcctccgc ccctcacctg gccaatgcca
721ccctggaaac cgagtggttc cacggcctgc ggcggaagtg gcggccacat ctgcacagaa
781gaggacccaa ccagggccct agaggcctgg gacacagctg gcggagaaag gatggcctgg
841gcggcgacaa gagccacttc aagtggtccc ccccctacct ggaatgcgag aacggcagct
901acaagcccgg ctggctggtc acactgagca gcgccatcta cggcctgcag cccaacctgg
961tgcccgagtt ccggggcgtg atgaaggtgg acatcaacct gcagaaagtg gacatcgacc
1021agtgcagcag cgacggctgg ttcagcggca cccacaagtg ccacctgaac aacagcgagt
1081gcatgcccat caagggcctg ggcttcgtgc tgggcgccta cgagtgcatc tgcaaggccg
1141gcttctacca ccccggcgtg ctgcccgtga acaacttcag acgcagaggc cccgatcagc
1201acatcagcgg cagcaccaag gacgtgtccg aagaggccta cgtgtgcctg ccctgcagag
1261agggctgccc cttctgcgcc gacgacagcc catgcttcgt gcaggaagat aagtacctgc
1321ggctggccat catcagtttt caggccctgt gcatgctgct ggacttcgtg tccatgctgg
1381tggtgtacca cttccggaag gccaagagca tccgggccag cggcctgatc ctgctggaaa
1441ccatcctgtt cggctcactg ctcctgtact tccccgtggt catcctgtac ttcgagccct
1501ccaccttccg gtgcatcctg ctgagatggg ccagactgct gggcttcgcc accgtgtacg
1561gcaccgtgac cctgaagctg caccgggtgc tgaaagtgtt cctgagccgg accgcccagc
1621ggatccccta catgacaggc ggcagagtga tgcggatgct ggctgtgatc ctcctggtcg
1681tgttctggtt cctgatcggc tggaccagca gcgtgtgcca gaacctggaa aagcagatca
1741gcctgatcgg ccagggcaag accagcgacc acctgatctt caacatgtgc ctgatcgacc
1801gctgggacta catgaccgcc gtggccgagt tcctgttcct gctgtggggc gtgtacctgt
1861gctacgccgt gcggacagtg cccagcgcct ttcacgagcc ccggtatatg gccgtggccg
1921tgcacaacga gctgatcatc tccgccatct tccacaccat cagattcgtg ctcgcttcta
1981gactgcagag cgactggatg ctgatgctgt actttgccca cacccacctg accgtgaccg
2041tgacaatcgg cctgctgctg atccccaagt tcagccacag cagcaacaac ccccgggacg
2101atatcgccac cgaggcctac gaggacgagc tggacatggg ccgcagcggc agctacctga
2161acagcagcat caacagcgct tggagcgagc acagcctgga ccccgaggac atccgggacg
2221agctgaagaa gctgtacgcc cagctggaaa tctacaagcg gaagaagatg atcaccaaca
2281accctcacct ccagaagaag cgctgcagca agaagggact gggccggtcc atcatgcggc
2341ggatcaccga gatccccgag acagtgtccc ggcagtgcag caaagaggac aaagagggcg
2401ccgaccacgg caccgccaag ggaacagccc tgatccggaa gaaccccccc gagagcagcg
2461gcaacaccgg caagtccaaa gaggaaaccc tgaagaaccg ggtgttcagc ctgaagaagt
2521cccacagcac ctacgaccac gtgcgggacc agaccgagga aagcagcagc ctgcccaccg
2581agagccagga agaggaaaca accgagaaca gcaccctgga atccctgagc ggcaagaagc
2641tgacccagaa gctgaaagag gacagcgagg ccgagagcac cgagagcgtg cccctcgtgt
2701gcaagagcgc ctccgcccac aacctgtcca gcgagaagaa aaccggccac ccccggacca
2761gcatgctgca gaaaagcctg agcgtgatcg ccagcgccaa agaaaagacc ctcggcctgg
2821ctggcaagac tcagacagcc ggcgtggaag agaggaccaa gagccagaag cccctgccca
2881aggacaaaga aacaaaccgg aaccacagca atagcgacaa caccgagaca aaggaccctg
2941ccccccagaa cagcaacccc gccgaggaac cccggaagcc ccagaaaagc ggcatcatga
3001agcagcagcg cgtgaacccc accaccgcca acagcgacct gaaccctggc accacccaga
3061tgaaggacaa cttcgacatc ggcgaagtgt gcccctggga ggtgtacgac ctgaccccag
3121gccccgtgcc cagcgagagc aaggtgcaga aacacgtgtc catcgtggcc agcgagatgg
3181aaaagaaccc cacctttagc ctgaaagaga agtctcacca caagcccaag gccgctgaag
3241tgtgccagca gagcaaccag aagcggatcg acaaggccga agtctgcctg tgggagagcc
3301agggccagag catcctggaa gatgagaagc tgctgattag caagaccccc gtgctgcctg
3361agcgggccaa agaagagaac ggcggccagc ccagggccgc caatgtctgt gccggccaga
3421gcgaggaact gccccctaaa gccgtggcca gcaagaccga gaacgagaac cTGAACCAGA
3481TCGGCCACCA GGAAAAGAAA ACCAGCAGCA GCGAGGAAAA CGTGCGGGGC TCCTACAACA
3541GCTCCAACAA CTTCCAGCAG CCCCTGACCA GCAGGGCTGA AGTCTGTCCA TGGGAATTCG
3601AGACACCCGC CCAGCCCAAC GCCGGCAGAT CTGTGGCCCT GCCTGCCAGC TCTGCCCTGA
3661GCGCCAACAA GATCGCCGGA CCCCGGAAAG AGGAAATCTG GGACAGCTTC AAAGTGTGAG
3721ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
3781GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
3841TTTATATATC TTGTGGAAAG GACGAACGCC ATCCGCCAAG CCGAGTGTGA CGCGTTAAGT
3901Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)

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