Construct: ORF TRCN0000488679
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020370.1_s317c1
- DNA Barcode:
- GCAGCCACATGCGCACTAATTAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SCYL2 (55681)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488679
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001317784.1 | 100% | 100% | |
| 2 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_017988.6 | 100% | 100% | |
| 3 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330253.1 | 99.5% | 99.5% | 1272_1283del |
| 4 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330254.1 | 99.5% | 99.5% | 1272_1283del |
| 5 | human | 55681 | SCYL2 | SCY1 like pseudokinase 2 | NM_001330256.1 | 81% | 81% | 0_1ins519;753_764del |
| 6 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_198021.2 | 88.7% | 93% | (many diffs) |
| 7 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | NM_001310704.1 | 88.7% | 92.9% | (many diffs) |
| 8 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_011243394.2 | 88.4% | 92.6% | (many diffs) |
| 9 | mouse | 213326 | Scyl2 | SCY1-like 2 (S. cerevisiae) | XM_006513458.3 | 88.3% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2859
- ORF length:
- 2787
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggagtcc atgcttaata aattgaagag tactgttaca aaagtaacag 121 ctgatgtcac tagtgctgta atgggaaatc ctgtcactag agaatttgat gttggtcgac 181 acattgccag tggtggcaat gggctagctt ggaagatttt taatggcaca aaaaagtcaa 241 caaagcagga agtggcagtt tttgtctttg ataaaaaact gattgacaag tatcaaaaat 301 ttgaaaagga tcaaatcatt gattctctaa aacgaggagt ccaacagtta actcggcttc 361 gacaccctcg acttcttact gtccagcatc ctttagaaga atccagggat tgcttggcat 421 tttgtacaga accagttttt gccagtttag ccaatgttct tggtaactgg gaaaatctac 481 cttcccctat atctccagac attaaggatt ataaacttta tgatgtagaa accaaatatg 541 gtttgcttca ggtttctgaa ggattgtcat tcttgcatag cagtgtgaaa atggtgcatg 601 gaaatatcac tcctgaaaat ataattttga ataaaagtgg agcctggaaa ataatgggtt 661 ttgatttttg tgtatcatca accaatcctt ctgaacaaga gcctaaattt ccttgtaaag 721 aatgggaccc aaatttacct tcattgtgtc ttccaaatcc tgaatatttg gctcctgaat 781 acatactttc tgtgagctgt gaaacagcca gtgatatgta ttctttagga actgttatgt 841 atgctgtatt taataaaggg aaacctatat ttgaagtcaa caagcaagat atttacaaga 901 gtttcagtag gcagttggat cagttgagtc gtttaggatc tagttcactt acaaatatac 961 ctgaggaagt tcgtgaacat gtaaagctac tgttaaatgt aactccgact gtaagaccag 1021 atgcagatca aatgacaaag attcccttct ttgatgatgt tggtgcagta acactgcaat 1081 attttgatac cttattccaa agagataatc ttcagaaatc acagtttttc aaaggactgc 1141 caaaggttct accaaaactg cccaagcgtg tcattgtgca gagaattttg ccttgtttga 1201 cttcagaatt tgtaaaccct gacatggtac cttttgtttt gcccaatgtt ctacttattg 1261 ctgaggaatg caccaaagaa gaatatgtca aattaattct tcctgaactt ggccctgtgt 1321 ttaagcagca ggagccaatc cagattttgt taattttcct acaaaaaatg gatttgctac 1381 taaccaaaac ccctcctgat gagataaaga acagtgttct acccatggtt tacagagcac 1441 tagaagctcc ttccattcag atccaggagc tctgtctaaa catcattcca acctttgcaa 1501 atcttataga ctacccatcc atgaaaaacg ctttgatacc aagaattaaa aatgcttgtc 1561 tacaaacatc ttcccttgcg gttcgtgtaa attcattagt gtgcttagga aagattttgg 1621 aatacttgga taagtggttt gtacttgatg atatcctacc cttcttacaa caaattccat 1681 ccaaggaacc tgcggtcctc atgggaattt taggtattta caaatgtact tttactcata 1741 agaagttggg aatcaccaaa gagcagctgg ccggaaaagt gttgcctcat cttattcccc 1801 tgagtattga aaacaatctt aatcttaatc agttcaattc tttcatttcc gtcataaaag 1861 aaatgcttaa tagattggag tctgaacata agactaaact ggagcaactt catataatgc 1921 aagaacagca gaaatctttg gatataggaa atcaaatgaa tgtttctgag gagatgaaag 1981 ttacaaatat tgggaatcag caaattgaca aagtttttaa caacattgga gcagaccttc 2041 tgactggcag tgagtccgaa aataaagagg acgggttaca gaataaacat aaaagagcat 2101 cacttacact tgaagaaaaa caaaaattag caaaagaaca agagcaggca cagaagctga 2161 aaagccagca gcctcttaaa ccccaagtgc acacacctgt tgctactgtt aaacagacta 2221 aggacttgac agacacactg atggataata tgtcatcctt gaccagcctt tctgttagta 2281 cccctaaatc ttctgcttca agtactttca cttctgttcc ttccatgggc attggtatga 2341 tgttttctac accaactgat aatacaaaga gaaatttgac aaatggccta aatgccaata 2401 tgggctttca gacttcagga ttcaacatgc ccgttaatac aaaccagaac ttctacagta 2461 gtccaagcac agttggagtg accaagatga ctctgggaac acctcccact ttgccaaact 2521 tcaatgcttt GAGTGTTCCT CCTGCTGGTG CAAAGCAGAC CCAACAAAGA CCCACAGATA 2581 TGTCTGCCCT TAATAATCTC TTTGGCCCTC AGAAACCCAA AGTTAGCATG AACCAGTTAT 2641 CACAACAGAA ACCAAATCAG TGGCTTAATC AGTTTGTACC TCCTCAAGGT TCTCCAACTA 2701 TGGGCAGTTC AGTAATGGGG ACACAGATGA ACGTGATAGG ACAATCTGCT TTTGGTATGC 2761 AGGGTAATCC TTTCTTTAAC CCACAGAACT TTGCACAGCC ACCAACTACT ATGACCAATA 2821 GCAGTTCAGC TAGCAATGAT TTAAAAGATC TTTTTGGGTA GGACCCAGCT TTCTTGTACA 2881 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2941 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3001 AGGACGAGCA GCCACATGCG CACTAATTAA TACGCGTTAA GTCgacaatc aacctctgga 3061 ttacaaaatt tgtgaaagat t