Construct: ORF TRCN0000488682
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020238.1_s317c1
- DNA Barcode:
- TCCTCACCAGAGTCAACCGTCCAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RPS6KA3 (6197)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488682
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | NM_004586.3 | 100% | 100% | |
| 2 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_005274573.2 | 99.8% | 99.8% | 1224_1225insCAG |
| 3 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545556.1 | 99% | 99% | 932_949del;1242_1243insCAG |
| 4 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545555.1 | 97.9% | 97.3% | (many diffs) |
| 5 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545563.3 | 96.2% | 96.2% | 0_1ins84 |
| 6 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029713.1 | 96.2% | 96.2% | 0_1ins84 |
| 7 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029714.2 | 96.2% | 96.2% | 0_1ins84 |
| 8 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029715.2 | 96.2% | 96.2% | 0_1ins84 |
| 9 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029716.1 | 96.2% | 96.2% | 0_1ins84 |
| 10 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029717.2 | 96.2% | 96.2% | 0_1ins84 |
| 11 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_006724507.3 | 96% | 96% | 0_1ins84;1140_1141insCAG |
| 12 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029719.2 | 96% | 96% | 0_1ins84;1140_1141insCAG |
| 13 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545557.2 | 95.4% | 95.4% | 0_1ins84;848_865del |
| 14 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545558.2 | 95.4% | 95.4% | 0_1ins84;848_865del |
| 15 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545560.1 | 95.4% | 95.4% | 0_1ins84;848_865del |
| 16 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545561.2 | 95.4% | 95.4% | 0_1ins84;848_865del |
| 17 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_017029718.2 | 94.7% | 94% | (many diffs) |
| 18 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_005274577.3 | 94.6% | 93.9% | (many diffs) |
| 19 | human | 6197 | RPS6KA3 | ribosomal protein S6 kinase A3 | XM_011545562.2 | 94% | 93.3% | (many diffs) |
| 20 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | NM_148945.2 | 94.4% | 99.8% | (many diffs) |
| 21 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_017318353.1 | 91.5% | 96.7% | (many diffs) |
| 22 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | NM_001346675.1 | 90.7% | 96% | (many diffs) |
| 23 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_006528687.1 | 90.7% | 96% | (many diffs) |
| 24 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_017318354.1 | 90.7% | 96% | (many diffs) |
| 25 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_017318355.1 | 90.7% | 96% | (many diffs) |
| 26 | mouse | 110651 | Rps6ka3 | ribosomal protein S6 kinase... | XM_006528688.2 | 89.3% | 94% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2292
- ORF length:
- 2220
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccgctg gcgcagctgg cggacccgtg gcagaagatg gctgtggaga 121 gcccgtccga cagcgctgag aatggacagc aaattatgga tgaacctatg ggagaggagg 181 agattaaccc acaaactgaa gaagtcagta tcaaagaaat tgcaatcaca catcatgtaa 241 aggaaggaca tgaaaaggca gatccttccc agtttgaact tttaaaagta ttagggcagg 301 gatcatttgg aaaggttttc ttagttaaaa aaatctcagg ctctgatgct aggcagcttt 361 atgccatgaa ggtattgaag aaggccacac tgaaagttcg agaccgagtt cggacaaaaa 421 tggaacgtga tatcttggta gaggttaatc atccttttat tgtcaagttg cattatgctt 481 ttcaaactga agggaagttg tatcttattt tggattttct caggggagga gatttgttta 541 cacgcttatc caaagaggtg atgttcacag aagaagatgt caaattctac ttggctgaac 601 ttgcacttgc tttagaccat ctacatagcc tgggaataat ttatagagac ttaaaaccag 661 aaaatatact tcttgatgaa gaaggtcaca tcaagttaac agatttcggc ctaagtaaag 721 agtctattga ccatgaaaag aaggcatatt ctttttgtgg aactgtggag tatatggctc 781 cagaagtagt taatcgtcga ggtcatactc agagtgctga ctggtggtct tttggtgtgt 841 taatgtttga aatgcttact ggtacactcc ctttccaagg aaaagatcga aaagaaacaa 901 tgactatgat tcttaaagcc aaacttggaa tgccacagtt tttgagtcct gaagcgcaga 961 gtcttttacg aatgcttttc aagcgaaatc ctgcaaacag attaggtgca ggaccagatg 1021 gagttgaaga aattaaaaga cattcatttt tctcaacgat agactggaat aaactgtata 1081 gaagagaaat tcatccgcca tttaaacctg caacgggcag gcctgaagat acattctatt 1141 ttgatcctga gtttactgca aaaactccca aagattcacc tggcattcca cctagtgcta 1201 atgcacatca gctttttcgg gggtttagtt ttgttgctat tacctcagat gatgaaagcc 1261 aagctatgca gacagttggt gtacattcaa ttgttcagca gttacacagg aacagtattc 1321 agtttactga tggatatgaa gtaaaagaag atattggagt tggctcctac tctgtttgca 1381 agagatgtat acataaagct acaaacatgg agtttgcagt gaagattatt gataaaagca 1441 agagagaccc aacagaagaa attgaaattc ttcttcgtta tggacagcat ccaaacatta 1501 tcactctaaa ggatgtatat gatgatggaa agtatgtgta tgtagtaaca gaacttatga 1561 aaggaggtga attgctggat aaaattctta gacaaaaatt tttctctgaa cgagaggcca 1621 gtgctgtcct gttcactata actaaaaccg ttgaatatct tcacgcacaa ggggtggttc 1681 atagagactt gaaacctagc aacattcttt atgtggatga atctggtaat ccggaatcta 1741 ttcgaatttg tgattttggc tttgcaaaac agctgagagc ggaaaatggt cttctcatga 1801 ctccttgtta cactgcaaat tttgttgcac cagaggtttt aaaaagacaa ggctatgatg 1861 ctgcttgtga tatatggagt cttggtgtcc tactctatac aatgcttacc ggttacactc 1921 catttgcaaa tggtccTGAT GATACACCAG AGGAAATATT GGCACGAATA GGTAGCGGAA 1981 AATTCTCACT CAGTGGTGGT TACTGGAATT CTGTTTCAGA CACAGCAAAG GACCTGGTGT 2041 CAAAGATGCT TCATGTAGAC CCTCATCAGA GACTGACTGC TGCTCTTGTG CTCAGACATC 2101 CTTGGATCGT CCACTGGGAC CAACTGCCAC AATACCAACT AAACAGACAG GATGCACCAC 2161 ATCTAGTAAA GGGTGCCATG GCAGCTACAT ATTCTGCTTT GAACCGTAAT CAGTCACCAG 2221 TTTTGGAACC AGTAGGCCGC TCTACTCTTG CTCAGCGGAG AGGTATTAAA AAAATCACCT 2281 CAACAGCCCT GTGAGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2341 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2401 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCCTCACCAG AGTCAACCGT 2461 CCATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt