Construct: ORF TRCN0000488712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020653.1_s317c1
- DNA Barcode:
- CGTTCACTCCCAGTCATCTCGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ROR2 (4920)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488712
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 4920 | ROR2 | receptor tyrosine kinase li... | NM_004560.4 | 99.6% | 99.8% | (many diffs) |
2 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_017014762.1 | 97.1% | 96% | (many diffs) |
3 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_005252008.4 | 84.9% | 85% | (many diffs) |
4 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_006717121.3 | 84.9% | 85% | (many diffs) |
5 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_017014763.1 | 84.9% | 85% | (many diffs) |
6 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_005252009.3 | 57.3% | 57.4% | 0_1ins1203;885C>T;1626_1627insTGATC |
7 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XR_001746315.1 | 46.6% | (many diffs) | |
8 | human | 4920 | ROR2 | receptor tyrosine kinase li... | NM_001318204.2 | 45.6% | 41.7% | (many diffs) |
9 | mouse | 26564 | Ror2 | receptor tyrosine kinase-li... | NM_013846.3 | 87.2% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 2931
- ORF length:
- 2829
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggccgccccc ttcacccaat tggcctgcgg catggctcga ggctcggcgc 121 tcccgcggcg gccgctgctg tgcatcccgg ccgtctgggc ggccgccgcg cttctgctct 181 cagtgtcccg gacttcaggt gaagtggagg ttctggatcc gaacgaccct ttaggacccc 241 ttgatgggca ggacggcccg attccaactc tgaaaggtta ctttctgaat tttctggagc 301 cagtaaacaa tatcaccatt gtccaaggcc agacggcaat tctgcactgc aaggtggcag 361 gaaacccacc ccctaacgtg cggtggctaa agaatgatgc cccggtggtg caggagccgc 421 ggcggatcat catccggaag acagaatatg gttcacgact gcgaatccag gacctggaca 481 cgacagacac tggctactac cagtgcgtgg ccaccaacgg gatgaagacc attaccgcca 541 ctggcgtcct gtttgtgcgg ctgggtccaa cgcacagccc aaatcataac tttcaggatg 601 attaccacga ggatgggttc tgccagcctt accggggaat tgcctgtgca cgcttcattg 661 gcaaccggac catttatgtg gactcgcttc agatgcaggg ggagattgaa aaccgaatca 721 cagcggcctt caccatgatc ggcacgtcta cgcacctgtc ggaccagtgc tcacagttcg 781 ccatcccatc cttctgccac ttcgtgtttc ctctgtgcga cgcgcgctcc cgggcaccca 841 agccgcgtga gctgtgccgc gacgagtgcg aggtgctgga gagcgacctg tgccgccagg 901 agtacaccat cgcccgctcc aacccgctca tcctcatgcg gcttcagctg cccaagtgtg 961 aggcgctgcc catgcctgag agccccgacg ctgccaactg catgcgcatt ggcatcccag 1021 ccgagaggct gggccgctac catcagtgct ataacggctc aggcatggat tacagaggaa 1081 cggcaagcac caccaagtca ggccaccagt gccagccgtg ggccctgcag cacccccaca 1141 gccaccacct gtccagcaca gacttccctg agcttggagg ggggcacgcc tactgccgga 1201 accccggagg ccagatggag ggcccctggt gctttacgca gaataaaaac gtacgcatgg 1261 aactgtgtga cgtaccctcg tgtagtcccc gagacagcag caagatgggg attctgtaca 1321 tcttggtccc cagcatcgca attccactgg tcatcgcttg ccttttcttc ttggtttgca 1381 tgtgccggaa taagcagaag gcatctgcgt ccacaccgca gcggcgacag ctgatggcct 1441 cgcccagcca agacatggaa atgcccctca ttaaccagca caaacaggcc aaactcaaag 1501 agatcagcct gtctgcggtg aggttcatgg aggagctggg agaggaccgg tttgggaaag 1561 tctacaaagg tcacctgttc ggccctgccc cgggggagca gacccaggct gtggccatca 1621 aaacgctgaa ggacaaagcg gaggggcccc tgcgggagga gttccggcat gaggctatgc 1681 tgcgagcacg gctgcaacac cccaacgtcg tctgcctgct gggcgtggtg accaaggacc 1741 agcccctgag catgatcttc agctactgtt cgcacggcga cctccacgaa ttcctggtca 1801 tgcgctcgcc gcactcggac gtgggcagca ccgatgatga ccgcacggtg aagtccgccc 1861 tggagccccc cgacttcgtg caccttgtgg cacagatcgc ggcggggatg gagtacctat 1921 ccagccacca cgtggttcac aaggacctgg ccacccgcaa tgtgctagtg tacgacaagc 1981 tgaacgtgaa gatctcagac ttgggcctct tccgagaggt gtatgccgcc gattactaca 2041 agctgctggg gaactcgctg ctgcctatcc gctggatggc cccagaggcc atcatgtacg 2101 gcaagttctc catcgactca gacatctggt cctacggtgt ggtcctgtgg gaggtcttca 2161 gctacggcct gcagccctac tgcgggtatt ccaaccagga tgtggtggag atgatccgga 2221 accggcaggt gctgccttgc cccgatgact gtcccgcctg ggtgtatgcc ctcatgatcg 2281 agtgctggaa cgagttcccc agccggcggc cccgcttcaa ggacatccac agccggctcc 2341 gagcctgggg caacctttcc aactacaaca gctcggcgca gacctcgggg gccagcaaca 2401 ccacgcagac cagctccctg agcaccagcc cagtgagcaa tgtgagcaac gcccgctacg 2461 tggggcccaa gcagaaggcc ccgcccttcc cacagcccca gttcatcccc atgaagggcc 2521 agatcagacc catggtgccc ccgccgcagc tctacgtccc cgtcaacggc taccagccgg 2581 tgccggccta tggggcctac ctgcccaact tctacccggt gcagatccca atgcagatgg 2641 ccccgcagca ggtgcctccT CAGATGGTCC CCAAGCCCAG CTCACACCAC AGTGGCAGTG 2701 GCTCCACCAG CACAGGCTAC GTCACCACGG CCCCCTCCAA CACATCCATG GCAGACAGGG 2761 CAGCCCTGCT CTCAGAGGGC GCTGATGACA CACAGAACGC CCCAGAAGAT GGGGCCCAGA 2821 GCACCGTGCA GGAAGCAGAG GAGGAGGAGG AAGGCTCTGT CCCAGAGACT GAGCTGCTGG 2881 GGGACTGTGA CACTCTGCAG GTGGACGAGG CCCAAGTCCA GCTGGAAGCT TGATCTAGAA 2941 AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 3001 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 3061 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CGTTCACTCC CAGTCATCTC 3121 GGTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt