Construct: ORF TRCN0000488714
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020534.1_s317c1
- DNA Barcode:
- CATTTGAGTGAGCTTGGATATAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TSSK1B (83942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488714
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83942 | TSSK1B | testis specific serine kina... | NM_032028.4 | 99.8% | 99.7% | 101T>C;1098C>A |
| 2 | mouse | 22114 | Tssk1 | testis-specific serine kina... | NM_009435.2 | 85.9% | 84.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1173
- ORF length:
- 1101
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggatgac gctgctgtcc tcaagcgacg aggctacctc ctggggataa 121 atttaggaga gggctcctat gcaaaagtaa aatctgctta ctctgagcgc ccgaagttca 181 atgtggcgat caagatcatc gaccgcaaga aggcccccgc agacttcttg gagaaattcc 241 ttccccggga aattgagatt ctggccatgt taaaccactg ctccatcatt aagacctacg 301 agatctttga gacatcacat ggcaaggtct acatcgtcat ggagctcgcg gtccagggcg 361 acctcctcga gttaatcaaa acccggggag ccctgcatga ggacgaagct cgcaagaagt 421 tccaccagct ttccttggcc atcaagtact gccacgacct ggacgtcgtc caccgggacc 481 tcaagtgtga caaccttctc cttgacaagg acttcaacat caagctgtcc gacttcagct 541 tctccaagcg ctgcctgcgg gatgacagtg gtcgaatggc attaagcaag accttctgtg 601 ggtcaccagc gtatgcggcc ccagaggtgc tgcagggcat tccctaccag cccaaggtgt 661 acgacatctg gagcctaggc gtgatcctct acatcatggt ctgcggctcc atgccctacg 721 acgactccaa catcaagaag atgctgcgta tccagaagga gcaccgcgtc aacttcccac 781 gctccaagca cctgacaggc gagtgcaagg acctcatcta ccacatgctg cagcccgacg 841 tcaaccggcg gctccacatc gacgagatcc TCAGCCACTG CTGGATGCAG CCCAAGGCAC 901 GGGGATCTCC CTCTGTGGCC ATCAACAAGG AGGGGGAGAG TTCCCGGGGA ACTGAACCCT 961 TGTGGACCCC CGAACCTGGC TCTGACAAGA AGTCTGCCAC CAAGCTGGAG CCTGAGGGAG 1021 AGGCACAGCC CCAGGCACAG CCTGAGACAA AACCCGAGGG GACAGCAATG CAAATGTCCA 1081 GGCAGTCGGA GATCCTGGGT TTCCCCAGCA AGCCGTCGAC TATGGAGACA GAGGAAGGGC 1141 CCCCCCAACA GCCTCCAGAG ACGCGGGCAC AGTAGAACCC AGCTTTCTTG TACAAAGTGG 1201 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1261 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGGAAAGGAC 1321 GACATTTGAG TGAGCTTGGA TATAAGACGC GTTAAGTCga caatcaacct ctggattaca 1381 aaatttgtga aagatt