Construct: ORF TRCN0000488715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020480.1_s317c1
- DNA Barcode:
- TGTGTCTGTAGTAGCGGAGAAAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ROR1 (4919)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488715
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4919 | ROR1 | receptor tyrosine kinase li... | NM_005012.4 | 99.8% | 99.8% | 180C>T;1170G>T;1553C>T |
| 2 | human | 4919 | ROR1 | receptor tyrosine kinase li... | XM_017001376.1 | 95% | 94.7% | (many diffs) |
| 3 | human | 4919 | ROR1 | receptor tyrosine kinase li... | XM_011541526.1 | 93.2% | 93.1% | 0_1ins189;981G>T;1364C>T |
| 4 | human | 4919 | ROR1 | receptor tyrosine kinase li... | XM_017001377.1 | 93.2% | 93.1% | 0_1ins189;981G>T;1364C>T |
| 5 | human | 4919 | ROR1 | receptor tyrosine kinase li... | NM_001083592.2 | 41.8% | 41.7% | (many diffs) |
| 6 | mouse | 26563 | Ror1 | receptor tyrosine kinase-li... | NM_013845.5 | 89.3% | 96.6% | (many diffs) |
| 7 | mouse | 26563 | Ror1 | receptor tyrosine kinase-li... | XM_006503121.2 | 71.4% | 78% | (many diffs) |
| 8 | mouse | 26563 | Ror1 | receptor tyrosine kinase-li... | NM_001312690.1 | 15.1% | 15.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2883
- ORF length:
- 2811
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcaccgg ccgcgccgcc gcgggacgcg cccgccgctc ctggcgctgc 121 tggccgcgct gctgctggcc gcacgcgggg ctgctgccca agaaacagag ctgtcagtca 181 gtgctgaatt agtgcctacc tcatcatgga acatctcaag tgaactcaac aaagattctt 241 acctgaccct tgatgaacca atgaataaca tcaccacgtc tctgggccag acagcagaac 301 tgcactgcaa agtctctggg aatccacctc ccaccatccg ctggttcaaa aatgatgctc 361 ctgtggtcca ggagccccgg aggctctcct ttcggtccac catctatggc tctcggctgc 421 ggattagaaa cctcgacacc acagacacag gctacttcca gtgcgtggca acaaacggca 481 aggaggtggt ttcttccact ggagtcttgt ttgtcaagtt tggcccccct cccactgcaa 541 gtccaggata ctcagatgag tatgaagaag atggattctg tcagccatac agagggattg 601 catgtgcaag atttattggc aaccgcaccg tctatatgga gtctttgcac atgcaagggg 661 aaatagaaaa tcagatcaca gctgccttca ctatgattgg cacttccagt cacttatctg 721 ataagtgttc tcagttcgcc attccttccc tgtgccacta tgccttcccg tactgcgatg 781 aaacttcatc cgtcccaaag ccccgtgact tgtgtcgcga tgaatgtgaa atcctggaga 841 atgtcctgtg tcaaacagag tacatttttg caagatcaaa tcccatgatt ctgatgaggc 901 tgaaactgcc aaactgtgaa gatctccccc agccagagag cccagaagct gcgaactgta 961 tccggattgg aattcccatg gcagatccta taaataaaaa tcacaagtgt tataacagca 1021 caggtgtgga ctaccggggg accgtcagtg tgaccaaatc agggcgccag tgccagccat 1081 ggaattccca gtatccccac acacacactt tcaccgccct tcgtttccca gagctgaatg 1141 gaggccattc ctactgccgc aacccaggga atcaaaagga agctccctgg tgcttcacct 1201 tggatgaaaa ctttaagtct gatctgtgtg acatcccagc ttgcgattca aaggattcca 1261 aggagaagaa taaaatggaa atcctgtaca tactagtgcc aagtgtggcc attcccctgg 1321 ccattgcttt actcttcttc ttcatttgcg tctgtcggaa taaccagaag tcatcgtcgg 1381 caccagtcca gaggcaacca aaacacgtca gaggtcaaaa tgtagagatg tcaatgctga 1441 atgcatataa acccaagagc aaggctaaag agctacctct ttctgctgta cgctttatgg 1501 aagaattggg tgagtgtgcc tttggaaaaa tctataaagg ccatctctat ctcccaggca 1561 tggaccatgc tcagctggtt gctatcaaga ccttgaaaga ctataacaac ccccagcaat 1621 ggatggaatt tcaacaagaa gcctccctaa tggcagaact gcaccacccc aatattgtct 1681 gccttctagg tgccgtcact caggaacaac ctgtgtgcat gctttttgag tatattaatc 1741 agggggatct ccatgagttc ctcatcatga gatccccaca ctctgatgtt ggctgcagca 1801 gtgatgaaga tgggactgtg aaatccagcc tggaccacgg agattttctg cacattgcaa 1861 ttcagattgc agctggcatg gaatacctgt ctagtcactt ctttgtccac aaggaccttg 1921 cagctcgcaa tattttaatc ggagagcaac ttcatgtaaa gatttcagac ttggggcttt 1981 ccagagaaat ttactccgct gattactaca gggtccagag taagtccttg ctgcccattc 2041 gctggatgcc ccctgaagcc atcatgtatg gcaaattctc ttctgattca gatatctggt 2101 cctttggggt tgtcttgtgg gagattttca gttttggact ccagccatat tatggattca 2161 gtaaccagga agtgattgag atggtgagaa aacggcagct cttaccatgc tctgaagact 2221 gcccacccag aatgtacagc ctcatgacag agtgctggaa tgagattcct tctaggagac 2281 caagatttaa agatattcac gtccggcttc ggtcctggga gggactctca agtcacacaa 2341 gctctactac tccttcaggg ggaaatgcca ccacacagac aacctccctc agtgccagcc 2401 cagtgagtaa tctcagtaac cccagatatc ctaattacat gttcccgagc cagggtatta 2461 caccacaggg ccagattgct ggtttcattg gcccgccaat acctcagaac cagcgattca 2521 ttcccatcaa tggataccca ataccTCCTG GATATGCAGC GTTTCCAGCT GCCCACTACC 2581 AGCCAACAGG TCCTCCCAGA GTGATTCAGC ACTGCCCACC TCCCAAGAGT CGGTCCCCAA 2641 GCAGTGCCAG TGGGTCGACT AGCACTGGCC ATGTGACTAG CTTGCCCTCA TCAGGATCCA 2701 ATCAGGAAGC AAATATTCCT TTACTACCAC ACATGTCAAT TCCAAATCAT CCTGGTGGAA 2761 TGGGTATCAC CGTTTTTGGC AACAAATCTC AAAAACCCTA CAAAATTGAC TCAAAGCAAG 2821 CATCTTTACT AGGAGACGCC AATATTCATG GACACACCGA ATCTATGATT TCTGCAGAAC 2881 TGTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2941 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 3001 CTTGGCTTTA TATATCTTGT GGAAAGGACG ATGTGTCTGT AGTAGCGGAG AAAGCACGCG 3061 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt