Construct: ORF TRCN0000488729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019439.1_s317c1
- DNA Barcode:
- CATTGTTCTAACATACGATATACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNR2 (1269)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1269 | CNR2 | cannabinoid receptor 2 | NM_001841.3 | 99.8% | 99.4% | 946C>T;1080_1081insG |
| 2 | human | 1269 | CNR2 | cannabinoid receptor 2 | XM_011540629.3 | 99.8% | 99.4% | 946C>T;1080_1081insG |
| 3 | human | 1269 | CNR2 | cannabinoid receptor 2 | XM_017000261.2 | 99.8% | 99.4% | 946C>T;1080_1081insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 63
- ORF end:
- 1146
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaagcca 61 ccatggagga atgctgggtg acagagatag ccaatggctc caaggatggc ttggattcca 121 accctatgaa ggattacatg atcctgagtg gtccccagaa gacagctgtt gctgtgttgt 181 gcactcttct gggcctgcta agtgccctgg agaacgtggc tgtgctctat ctgatcctgt 241 cctcccacca actccgccgg aagccctcat acctgttcat tggcagcttg gctggggctg 301 acttcctggc cagtgtggtc tttgcatgca gctttgtgaa tttccatgtt ttccatggtg 361 tggattccaa ggctgtcttc ctgctgaaga ttggcagcgt gactatgacc ttcacagcct 421 ctgtgggtag cctcctgctg accgccattg accgatacct ctgcctgcgc tatccacctt 481 cctacaaagc tctgctcacc cgtggaaggg cactggtgac cctgggcatc atgtgggtcc 541 tctcagcact agtctcctac ctgcccctca tgggatggac ttgctgtccc aggccctgct 601 ctgagctttt cccactgatc cccaatgact acctgctgag ctggctcctg ttcatcgcct 661 tcctcttttc cggaatcatc tacacctatg ggcatgttct ctggaaggcc catcagcatg 721 tggccagctt gtctggccac caggacaggc aggtgccagg aatggcccga atgaggctgg 781 atgtgaggtt ggccaagacc ctagggctag tgttGGCTGT GCTCCTCATC TGTTGGTTCC 841 CAGTGCTGGC CCTCATGGCC CACAGCCTGG CCACTACGCT CAGTGACCAG GTCAAGAAGG 901 CCTTTGCTTT CTGCTCCATG CTGTGCCTCA TCAACTCCAT GGTCAACCCT GTCATCTATG 961 CTCTACGGAG TGGAGAGATC CGCTCCTCTG CCCATCACTG CCTGGCTTAC TGGAAGAAGT 1021 GTGTGAGGGG CCTTGGGTCA GAGGCAAAAG AAGAAGCCCC GAGATCCTCA GTCACCGAGA 1081 CAGAGGCTGA TGGGAAAATC ACTCCGTGGC CAGATTCCAG AGATCTAGAC CTCTCTGATT 1141 GCGACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGACA TTGTTCTAAC ATACGATATA CTACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt