Construct: ORF TRCN0000488733
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021858.1_s317c1
- DNA Barcode:
- CCCGCACGCAAACTACATTCGTCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TP53BP2 (7159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488733
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | NM_005426.2 | 100% | 100% | |
| 2 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | XM_011544269.2 | 100% | 100% | |
| 3 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | XM_011544268.2 | 94.1% | 94.1% | 1_186del |
| 4 | human | 7159 | TP53BP2 | tumor protein p53 binding p... | NM_001031685.3 | 88.6% | 88.6% | 1_387del |
| 5 | mouse | 209456 | Trp53bp2 | transformation related prot... | NM_173378.2 | 76.6% | 80% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3084
- ORF length:
- 3015
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggatctgact cttgctgaac ttcaggaaat ggcatctcgc cagcagcaac 121 agattgaagc ccagcaacaa ttgctggcaa ctaaggaaca gcgcttaaag tttttgaaac 181 aacaagatca gcgacaacag caacaagttg ctgagcagga gaaacttaaa aggctaaaag 241 aaatagctga gaatcaggaa gctaagctaa aaaaagtgag agcacttaaa ggccacgtgg 301 aacagaagag actaagcaat gggaaacttg tggaggaaat tgaacagatg aataatttgt 361 tccagcaaaa acagagggag ctcgtcctgg ctgtgtcaaa agtagaagaa ctgaccaggc 421 agctagagat gctcaagaac ggcaggatcg acagccacca tgacaatcag tctgcagtgg 481 ctgagcttga tcgcctctat aaggagctgc agctaagaaa caaattgaat caagagcaga 541 atgccaagct acaacaacag agggagtgtt tgaataagcg taattcagaa gtggcagtca 601 tggataagcg tgttaatgag ctgagggacc ggctgtggaa gaagaaggca gctctacagc 661 aaaaagaaaa tctaccagtt tcatctgatg gaaatcttcc ccagcaagcc gcgtcagccc 721 caagccgtgt ggctgcagta ggtccctata tccagtcgtc tactatgcct cggatgccct 781 caaggcctga attgctggtg aagccagccc tgccggatgg ttccttggtc attcaggctt 841 cagaggggcc gatgaaaata cagacactgc ccaacatgag atctggggct gcttcacaaa 901 ctaaaggctc taaaatccat ccagttggcc ctgattggag tccttcaaat gcagatcttt 961 tcccaagcca aggctctgct tctgtacctc aaagcactgg gaatgctctg gatcaagttg 1021 atgatggaga ggttccgctg agggagaaag agaagaaagt gcgtccgttc tcaatgtttg 1081 atgcagtaga ccagtccaat gccccacctt cctttggtac tctgaggaag aaccagagca 1141 gtgaagatat cttgcgggat gctcaggttg caaataaaaa tgtggctaaa gtaccacctc 1201 ctgttcctac aaaaccaaaa cagattaatt tgccttattt tggacaaact aatcagccac 1261 cttcagacat taagccagac ggaagttctc agcagttgtc aacagttgtt ccgtccatgg 1321 gaactaaacc aaaaccagca gggcagcagc cgagagtgct gctatctccc agcatacctt 1381 cggttggcca agaccagacc ctttctccag gttctaagca agaaagtcca cctgctgctg 1441 ccgtccggcc ctttactccc cagccttcca aagacacctt acttccaccc ttcagaaaac 1501 cccagaccgt ggcagcaagt tcaatatatt ccatgtatac gcaacagcag gcgccaggaa 1561 aaaacttcca gcaggctgtg cagagcgcgt tgaccaagac tcataccaga gggccacact 1621 tttcaagtgt atatggtaag cctgtaattg ctgctgccca gaatcaacag cagcacccag 1681 agaacattta ttccaatagc cagggcaagc ctggcagtcc agaacctgaa acagagcctg 1741 tttcttcagt tcaggagaac catgaaaacg aaagaattcc tcggccactc agcccaacta 1801 aattactgcc tttcttatct aatccttacc gaaaccagag tgatgctgac ctagaagcct 1861 tacgaaagaa actgtctaac gcaccaaggc ctctaaagaa acgtagttct attacagagc 1921 cagagggtcc taatgggcca aatattcaga agcttttata tcagaggacc accatagcgg 1981 ccatggagac catctctgtc ccatcatacc catccaagtc agcttctgtg actgccagct 2041 cagaaagccc agtagaaatc cagaatccat atttacatgt ggagcccgaa aaggaggtgg 2101 tctctctggt tcctgaatca ttgtccccag aggatgtggg gaatgccagt acagagaaca 2161 gtgacatgcc agctccttct ccaggccttg attatgagcc tgagggagtc ccagacaaca 2221 gcccaaatct ccagaataac ccagaagaac caaatccaga ggctccacat gtgcttgatg 2281 tgtacctgga ggagtaccct ccatacccac ccccaccata cccatctggg gagcctgaag 2341 ggcccggaga agactcggtg agcatgcgcc cgcctgaaat caccgggcag gtctctctgc 2401 ctcctggtaa aaggacaaac ttgcgtaaaa ctggctcaga gcgtatcgct catggaatga 2461 gggtgaaatt caaccccctt gctttactgc tagattcgtc tttggaggga gaatttgacc 2521 ttgtacagag aattatttat gaggttgatg acccaagcct ccccaatgat gaaggcatca 2581 cggctcttca caatgctgtg tgtgcaggcc acacagaaat cgttaagttc ctggtacagt 2641 ttggtgtaaa tgtaaatgct gctgatagtg atggatggac tccattacat tgtgctgcct 2701 catgtaacaa cgtccaagtg tgtaagtttt tggtggagtc aggagccgct gtgtttgcca 2761 tgaccTACAG TGACATGCAG ACTGCTGCAG ATAAGTGCGA GGAAATGGAG GAAGGCTACA 2821 CTCAGTGCTC CCAATTTCTT TATGGAGTTC AGGAGAAGAT GGGCATAATG AATAAAGGAG 2881 TCATTTATGC GCTTTGGGAT TATGAACCTC AGAATGATGA TGAGCTGCCC ATGAAAGAAG 2941 GAGACTGCAT GACAATCATC CACAGGGAAG ACGAAGATGA AATCGAATGG TGGTGGGCGC 3001 GCCTTAATGA TAAGGAGGGA TATGTTCCAC GTAACTTGCT GGGACTGTAC CCAAGAATTA 3061 AACCAAGACA AAGGAGCTTG GCCTGAGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3121 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3181 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCCGCACG 3241 CAAACTACAT TCGTCAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 3301 aagatt