Construct: ORF TRCN0000488734
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021698.1_s317c1
- DNA Barcode:
- ATCCTGCTGGTAACCGGCATACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ROR2 (4920)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488734
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_005252009.3 | 92% | 92% | 1_129del;885C>T |
| 2 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_005252008.4 | 62.1% | 62.1% | 1_912del;1668C>T |
| 3 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_006717121.3 | 62.1% | 62.1% | 1_912del;1668C>T |
| 4 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_017014763.1 | 62.1% | 62.1% | 1_912del;1668C>T |
| 5 | human | 4920 | ROR2 | receptor tyrosine kinase li... | XM_017014762.1 | 53% | 53% | 1_1323del;2079C>T |
| 6 | human | 4920 | ROR2 | receptor tyrosine kinase li... | NM_004560.4 | 52.8% | 52.9% | 1_1332del;2088C>T |
| 7 | mouse | 26564 | Ror2 | receptor tyrosine kinase-li... | NM_013846.3 | 46.8% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1566
- ORF length:
- 1497
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttgat ggcctcgccc agccaagaca tggaaatgcc cctcattaac cagcacaaac 121 aggccaaact caaagagatc agcctgtctg cggtgaggtt catggaggag ctgggagagg 181 accggtttgg gaaagtctac aaaggtcacc tgttcggccc tgccccgggg gagcagaccc 241 aggctgtggc catcaaaacg ctgaaggaca aagcggaggg gcccctgcgg gaggagttcc 301 ggcatgaggc tatgctgcga gcacggctgc aacaccccaa cgtcgtctgc ctgctgggcg 361 tggtgaccaa ggaccagccc ctgagcatga tcttcagcta ctgttcgcac ggcgacctcc 421 acgaattcct ggtcatgcgc tcgccgcact cggacgtggg cagcaccgat gatgaccgca 481 cggtgaagtc cgccctggag ccccccgact tcgtgcacct tgtggcacag atcgcggcgg 541 ggatggagta cctatccagc caccacgtgg ttcacaagga cctggccacc cgcaatgtgc 601 tagtgtacga caagctgaac gtgaagatct cagacttggg cctcttccga gaggtgtatg 661 ccgccgatta ctacaagctg ctggggaact cgctgctgcc tatccgctgg atggccccag 721 aggccatcat gtacggcaag ttctccatcg actcagacat ctggtcctac ggtgtggtcc 781 tgtgggaggt cttcagctac ggcctgcagc cctactgcgg gtattccaac caggatgtgg 841 tggagatgat ccggaaccgg caggtgctgc cttgccccga tgactgtccc gcctgggtgt 901 atgccctcat gatcgagtgc tggaacgagt tccccagccg gcggccccgc ttcaaggaca 961 tccacagccg gctccgagcc tggggcaacc tttccaacta caacagctcg gcgcagacct 1021 cgggggccag caacaccacg cagaccagct ccctgagcac cagcccagtg agcaatgtga 1081 gcaacgcccg ctacgtgggg cccaagcaga aggccccgcc cttcccacag ccccagttca 1141 tccccatgaa gggccagatc agacccatgg tgcccccgcc gcagctctac gtccccgtca 1201 acggctacca gccggtgccg gcctatgggg cctacctgcc caacttctac ccggtgcaga 1261 tcccaatgca gatggccccg cagcaggtgc ctccTCAGAT GGTCCCCAAG CCCAGCTCAC 1321 ACCACAGTGG CAGTGGCTCC ACCAGCACAG GCTACGTCAC CACGGCCCCC TCCAACACAT 1381 CCATGGCAGA CAGGGCAGCC CTGCTCTCAG AGGGCGCTGA TGACACACAG AACGCCCCAG 1441 AAGATGGGGC CCAGAGCACC GTGCAGGAAG CAGAGGAGGA GGAGGAAGGC TCTGTCCCAG 1501 AGACTGAGCT GCTGGGGGAC TGTGACACTC TGCAGGTGGA CGAGGCCCAA GTCCAGCTGG 1561 AAGCTTGAGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1621 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1681 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCCTG CTGGTAACCG GCATACCTAC 1741 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt