Construct: ORF TRCN0000488739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021282.1_s317c1
- DNA Barcode:
- GATTGGCCCTAAGCTGTACAACAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SPPL2B (56928)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_011528140.1 | 29.8% | 30% | 120T>C;129T>C;352_1170del |
| 2 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_024451619.1 | 26.4% | 10.6% | (many diffs) |
| 3 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027019.1 | 26.2% | 26.3% | (many diffs) |
| 4 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027018.2 | 25.2% | 25.4% | (many diffs) |
| 5 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_011528139.2 | 25.2% | 25.3% | (many diffs) |
| 6 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027017.1 | 25.2% | 25.3% | (many diffs) |
| 7 | human | 56928 | SPPL2B | signal peptide peptidase li... | NM_001077238.2 | 22.7% | 22.8% | (many diffs) |
| 8 | human | 56928 | SPPL2B | signal peptide peptidase li... | NM_152988.3 | 19.6% | 19.7% | (many diffs) |
| 9 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753722.1 | 19.3% | (many diffs) | |
| 10 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_011528138.2 | 18.8% | 18.9% | (many diffs) |
| 11 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753719.1 | 18.4% | (many diffs) | |
| 12 | human | 56928 | SPPL2B | signal peptide peptidase li... | XM_017027016.1 | 18% | 13.9% | (many diffs) |
| 13 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_002958334.1 | 9.4% | (many diffs) | |
| 14 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753720.2 | 8.9% | (many diffs) | |
| 15 | human | 56928 | SPPL2B | signal peptide peptidase li... | XR_001753721.1 | 8.8% | (many diffs) | |
| 16 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_006514215.2 | 23.9% | 24.4% | (many diffs) |
| 17 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_017314120.1 | 23.7% | 24.2% | (many diffs) |
| 18 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_006514214.2 | 20% | 20.3% | (many diffs) |
| 19 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_006514213.3 | 19.4% | 19.8% | (many diffs) |
| 20 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XR_380447.3 | 19.3% | (many diffs) | |
| 21 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_006514212.2 | 18.8% | 19.1% | (many diffs) |
| 22 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_006514210.2 | 17.5% | 17.8% | (many diffs) |
| 23 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | NM_175195.3 | 17.4% | 17.8% | (many diffs) |
| 24 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_017314119.1 | 17.4% | 17.8% | (many diffs) |
| 25 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XM_006514209.2 | 17.4% | 17.7% | (many diffs) |
| 26 | mouse | 73218 | Sppl2b | signal peptide peptidase li... | XR_001779612.1 | 9.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 423
- ORF length:
- 351
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaagcac aagcgcgacg atgggcccga gaagcaggag gacgaggcgg 121 tggacgtgac gccggtgatg acctgcgtgt ttgtggtgat gtgctgctcc atgctggtgc 181 tgctctacta cttcTACGAC CTCCTCGTGT ACGTGGTCAT CGGGATCTTC TGCCTGGCCT 241 CCGCCACCGG CCTCTACAGC TGCCTGGCGC CCTGTGTGCG GCGGCTGCCC TTCGGCAAGT 301 GCAGGATCCC CAACAACAGC CTGCCCTACT TCCACAAGCG CCCGCAGGCC CGTATGCTGC 361 TCCTGGCGCT CTTCTGCGTG GCCGTCAGCG TGGTGTGGGG CGTCTTCCGC AACGAGGACC 421 AGTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGATTGGCCC TAAGCTGTAC AACAGACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt