Construct: ORF TRCN0000488744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020843.1_s317c1
- DNA Barcode:
- TAGCGAAATGGCGTACTGACCCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GPRC5D (55507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55507 | GPRC5D | G protein-coupled receptor ... | NM_018654.1 | 99.8% | 100% | 714C>T;987T>C |
| 2 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_005253421.3 | 99.8% | 100% | 714C>T;987T>C |
| 3 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019582.2 | 99.8% | 100% | 714C>T;987T>C |
| 4 | human | 55507 | GPRC5D | G protein-coupled receptor ... | XM_017019583.2 | 86.7% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1107
- ORF length:
- 1035
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtacaag gactgcatcg agtccactgg agactatttt cttctctgtg 121 acgccgaggg gccatggggc atcattctgg agtccctggc catacttggc atcgtggtca 181 caattctgct actcttagca tttctcttcc tcatgcgaaa gatccaagac tgcagccagt 241 ggaatgtcct ccccacccag ctcctcttcc tcctgagtgt cctggggctc ttcggactcg 301 cttttgcctt catcatcgag ctcaatcaac aaactgcccc cgtacgctac tttctctttg 361 gggttctctt tgctctctgt ttctcatgcc tcttagctca tgcctccaat ctagtgaagc 421 tggttcgggg ttgtgtctcc ttctcctgga cgacaattct gtgcattgct attggttgca 481 gtctgttgca aatcattatt gccactgagt atgtgactct catcatgacc agaggtatga 541 tgtttgtgaa tatgacaccc tgccagctca atgtggactt tgttgtactc ctggtctatg 601 tcctcttcct gatggccctc acattcttcg tctccaaagc caccttctgt ggcccgtgtg 661 agaactggaa gcagcatgga aggctcatct ttatcactgt gctcttctcc atcatcatct 721 gggtggtgtg gatctccatg ctccTGAGAG GCAACCCGCA GTTCCAGCGA CAGCCCCAGT 781 GGGATGACCC GGTCGTCTGC ATTGCTCTGG TCACCAACGC ATGGGTTTTC CTGCTGCTGT 841 ACATCGTCCC TGAGCTCTGC ATTCTCTACA GATCGTGTAG ACAGGAGTGC CCTTTACAAG 901 GCAATGCCTG CCCCGTCACA GCCTACCAAC ACAGCTTCCA AGTGGAGAAC CAGGAGCTCT 961 CCAGAGCCCG AGACAGTGAT GGAGCTGAGG AGGATGTAGC ATTAACTTCA TATGGTACTC 1021 CCATTCAGCC GCAGACTGTT GATCCCACAC AAGAGTGCTT CATCCCACAG GCTAAACTAA 1081 GCCCCCAGCA AGATGCAGGA GGAGTATAGG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1141 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1201 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATAGCG 1261 AAATGGCGTA CTGACCCGCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1321 tgaaagatt