Construct: ORF TRCN0000488748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020483.1_s317c1
- DNA Barcode:
- CTATGATATAATGGTCTGGCCCTG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PKN1 (5585)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5585 | PKN1 | protein kinase N1 | NM_002741.5 | 99.9% | 99.8% | 771A>G;2701G>A |
| 2 | human | 5585 | PKN1 | protein kinase N1 | NM_213560.3 | 98.9% | 98.5% | (many diffs) |
| 3 | mouse | 320795 | Pkn1 | protein kinase N1 | NM_177262.4 | 85.1% | 91.5% | (many diffs) |
| 4 | mouse | 320795 | Pkn1 | protein kinase N1 | NM_001199593.1 | 84.5% | 90.7% | (many diffs) |
| 5 | mouse | 320795 | Pkn1 | protein kinase N1 | XR_001778447.1 | 66.7% | (many diffs) | |
| 6 | mouse | 320795 | Pkn1 | protein kinase N1 | XR_001778448.1 | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2898
- ORF length:
- 2826
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggccagc gacgccgtgc agagtgagcc tcgcagctgg tccctgctag 121 agcagctggg cctggccggg gcagacctgg cggcccccgg ggtacagcag cagctggagc 181 tggagcggga gcggctgcgg cgggaaatcc gcaaggagct gaagctgaag gagggtgctg 241 agaacctgcg gcgggccacc actgacctgg gccgcagcct gggccccgta gagctgctgc 301 tgcggggctc ctcgcgccgc ctcgacctgc tgcaccagca gctgcaggag ctgcacgccc 361 acgtggtgct tcccgacccg gcggccaccc acgatggccc ccagtcccct ggtgcgggtg 421 gccccacctg ctcggccacc aacctgagcc gcgtggcggg cctggagaag cagttggcca 481 ttgagctgaa ggtgaagcag ggggcggaga acatgatcca gacctacagc aatggcagca 541 ccaaggaccg gaagctgctg ctgacagccc agcagatgtt gcaggacagt aagaccaaga 601 ttgacatcat ccgcatgcaa ctccgccggg cgctgcaggc cggccagctg gagaaccagg 661 cagccccgga tgacacccaa gggagtcctg acctgggggc tgtggagctg cgcatcgaag 721 agctgcggca ccacttccga gtggagcacg cggtggccga gggtgccaag aacgtactgc 781 gcctgctcag cgctgccaag gccccggacc gcaaggcagt cagcgaggcc caggagaaat 841 tgacagaatc caaccagaag ctggggctgc tgcgggaggc tctggagcgg agacttgggg 901 agctgcccgc cgaccacccc aaggggcggc tgctgcgaga agagctcgct gcggcctcct 961 ccgctgcctt cagcacccgc ctggccgggc cctttcccgc cacgcactac agcaccctgt 1021 gcaagcccgc gccgctcaca gggaccctgg aggtacgagt ggtgggctgc agagacctcc 1081 cagagaccat cccgtggaac cctaccccct caatgggggg acctgggacc ccagacagcc 1141 gccccccctt cctgagccgc ccagcccggg gcctttacag ccgaagcgga agcctcagtg 1201 gccggagcag cctcaaagca gaagccgaga acaccagtga agtcagcact gtgcttaagc 1261 tggataacac agtggtgggg cagacgtctt ggaagccatg tggccccaat gcctgggacc 1321 agagcttcac tctggagctg gaaagggcac gggaactgga gttggctgtg ttctggcggg 1381 accagcgggg cctgtgtgcc ctcaaattcc tgaagttgga ggatttcttg gacaatgaga 1441 ggcatgaggt gcagctggac atggaacccc agggctgcct ggtggctgag gtcaccttcc 1501 gcaaccctgt cattgagagg attcctcggc tccgacggca gaagaaaatt ttctccaagc 1561 agcaagggaa ggcgttccag cgtgctaggc agatgaacat cgatgtcgcc acgtgggtgc 1621 ggctgctccg gaggctcatc cccaatgcca cgggcacagg cacctttagc cctggggctt 1681 ctccaggatc cgaggcccgg accacgggtg acatatcggt ggagaagctg aacctcggca 1741 ctgactcgga cagctcacct cagaagagct cgcgggatcc tccttccagc ccatcgagcc 1801 tgagctcccc catccaggaa tccactgctc ccgagctgcc ttcggagacc caggagaccc 1861 caggccccgc cctgtgcagc cctctgagga agtcacctct gaccctcgaa gatttcaagt 1921 tcctggcggt gctgggccgg ggtcattttg ggaaggtgct cctctccgaa ttccggccca 1981 gtggggagct gttcgccatc aaggctctga agaaagggga cattgtggcc cgagacgagg 2041 tggagagcct gatgtgtgag aagcggatat tggcggcagt gaccagtgcg ggacacccct 2101 tcctggtgaa cctcttcggc tgtttccaga caccggagca cgtgtgcttc gtgatggagt 2161 actcggccgg tggggacctg atgctgcaca tccacagcga cgtgttctct gagccccgtg 2221 ccatctttta ttccgcctgc gtggtgctgg gcctacagtt tcttcacgaa cacaagatcg 2281 tctacaggga cctgaagttg gacaatttgc tcctggacac cgagggctac gtcaagatcg 2341 cagactttgg cctctgcaag gaggggatgg gctatgggga ccggaccagc acattctgtg 2401 ggaccccgga gttcctggcc cctgaggtgc tgacggacac gtcgtacacg cgagctgtgg 2461 actggtgggg actgggtgtg ctgctctacg agatgctggt tggcgagtcc ccattcccag 2521 gggatgatga ggaggaggtc ttcgacagca tcgtcaacga cgaggttcgc tacccccgct 2581 tcctgtcggc cgaagccatc ggcatcatga gaaggctgct tcGGAGGAAC CCAGAGCGGA 2641 GGCTGGGATC TAGCGAGAGA GATGCAGAAG ATGTGAAGAA ACAGCCCTTC TTCAGGACTC 2701 TGGGCTGGGA AGCCCTGTTG GCCCGGCGCC TGCCACCGCC CTTTGTGCCC ACGCTGTCCG 2761 GCCGCACCGA CATCAGCAAC TTCGACGAGG AGTTCACCGG GGAGGCCCCC ACACTGAGCC 2821 CGCCCCGCGA CGCGCGGCCC CTCACAGCCG CGGAGCAGGC AGCCTTCCTG GACTTCGACT 2881 TCGTGGCCGG GGGCTGCTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2941 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3001 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTAT GATATAATGG 3061 TCTGGCCCTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt