Construct: ORF TRCN0000488769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021279.1_s317c1
- DNA Barcode:
- AGCATAAATAGTGTATAGTGGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TRPM8 (79054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79054 | TRPM8 | transient receptor potentia... | NM_024080.5 | 16.3% | 15.3% | (many diffs) |
| 2 | human | 79054 | TRPM8 | transient receptor potentia... | XM_011511810.2 | 16.1% | 15.2% | (many diffs) |
| 3 | human | 79054 | TRPM8 | transient receptor potentia... | XM_024453132.1 | 15.2% | 14.8% | (many diffs) |
| 4 | human | 79054 | TRPM8 | transient receptor potentia... | XM_017004891.1 | 15.1% | 14.6% | (many diffs) |
| 5 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_017319118.1 | 21.8% | 22.3% | (many diffs) |
| 6 | mouse | 171382 | Trpm8 | transient receptor potentia... | NM_134252.3 | 14.4% | 14.6% | (many diffs) |
| 7 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529225.1 | 14.4% | 14.6% | (many diffs) |
| 8 | mouse | 171382 | Trpm8 | transient receptor potentia... | XM_006529226.1 | 14.4% | 14.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 648
- ORF length:
- 576
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaatcc ttccttcctg tccacaccat cgtgcttatc agggagaatg 121 tgtgcaagtg tggctatgcc cagagccagc acatggaagg cacccagatc aaccaaagtg 181 agaaatggaa ctacaagaaa cacaccaagg aatttcctac cgacgccttt ggggatattc 241 agtttgagac actggggaag aaagggaagt atatacgtct gtccTGCGAC ACGGACGCGG 301 AAATCCTTTA CGAGCTGCTG ACCCAGCACT GGCACCTGAA AACACCCAAC CTGGTCATTT 361 CTGTGACCGG GGGCGCCAAG AACTTCGCCC TGAAGCCGCG CATGCGCAAG ATCTTCAGCC 421 GGCTCATCTA CATCGCGCAG TCCAAAGGTG CTTGGATTCT CACGGGAGGC ACCCATTATG 481 GCCTGATGAA GTACATCGGG GAGGTGGTGA GAGATAACAC CATCAGCAGG AGTTCAGAGG 541 AGAATATTGT GGCCATTGGC ATAGCAGCTT GGGGCATGGT CTCCAACCGG GACACCCTCA 601 TCAGGAATTG CGATGCTGAG GTACCGGTGG GACAGGAGGA GGTCTGCTAG AACCCAGCTT 661 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 721 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 781 CTTGTGGAAA GGACGAAGCA TAAATAGTGT ATAGTGGCGT ACGCGTTAAG TCgacaatca 841 acctctggat tacaaaattt gtgaaagatt