Construct: ORF TRCN0000488789
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019356.1_s317c1
- DNA Barcode:
- CTTACGCTACCTCCATTTCTGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CXCR4 (7852)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488789
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_003467.3 | 99.9% | 99.7% | 1056_1057insG |
| 2 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001008540.2 | 96.9% | 97.1% | (many diffs) |
| 3 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348060.2 | 95.6% | 95.4% | 0_1ins45;1011_1012insG |
| 4 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348059.2 | 91.3% | 90.1% | 16_114del;1155_1156insG |
| 5 | human | 7852 | CXCR4 | C-X-C motif chemokine recep... | NM_001348056.2 | 81.4% | 81.8% | 1_225del;228_229insGAGGGGATCAGT;1269_1270insG |
| 6 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | XM_006529113.3 | 85.5% | 89.6% | (many diffs) |
| 7 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | NM_009911.3 | 85% | 88.8% | (many diffs) |
| 8 | mouse | 12767 | Cxcr4 | chemokine (C-X-C motif) rec... | XM_006529114.2 | 84.7% | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1134
- ORF length:
- 1059
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatggag gggatcagta tatacacttc agataactac accgaggaaa 121 tgggctcagg ggactatgac tccatgaagg aaccctgttt ccgtgaagaa aatgctaatt 181 tcaataaaat cttcctgccc accatctact ccatcatctt cttaactggc attgtgggca 241 atggattggt catcctggtc atgggttacc agaagaaact gagaagcatg acggacaagt 301 acaggctgca cctgtcagtg gccgacctcc tctttgtcat cacgcttccc ttctgggcag 361 ttgatgccgt ggcaaactgg tactttggga acttcctatg caaggcagtc catgtcatct 421 acacagtcaa cctctacagc agtgtcctca tcctggcctt catcagtctg gaccgctacc 481 tggccatcgt ccacgccacc aacagtcaga ggccaaggaa gctgttggct gaaaaggtgg 541 tctatgttgg cgtctggatc cctgccctcc tgctgactat tcccgacttc atctttgcca 601 acgtcagtga ggcagatgac agatatatct gtgaccgctt ctaccccaat gacttgtggg 661 tggttgtgtt ccagtttcag cacatcatgg ttggccttat cctgcctggt attgtcatcc 721 tgtcctgcta ttgcattatc atctccaagc tgtcacactc caagggccac cagaagcgca 781 aggccctcaa gaccacagtc atcctcatcc tggcttTCTT CGCCTGTTGG CTGCCTTACT 841 ACATTGGGAT CAGCATCGAC TCCTTCATCC TCCTGGAAAT CATCAAGCAA GGGTGTGAGT 901 TTGAGAACAC TGTGCACAAG TGGATTTCCA TCACCGAGGC CCTAGCTTTC TTCCACTGTT 961 GTCTGAACCC CATCCTCTAT GCTTTCCTTG GAGCCAAATT TAAAACCTCT GCCCAGCACG 1021 CACTCACCTC TGTGAGCAGA GGGTCCAGCC TCAAGATCCT CTCCAAAGGA AAGCGAGGTG 1081 GACATTCATC TGTTTCCACT GAGTCTGAGT CTTCAAGTTT TCACTCCAGC GACCCAGCTT 1141 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1201 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1261 CTTGTGGAAA GGACGACTTA CGCTACCTCC ATTTCTGGAC ACGCGTTAAG TCgacaatca 1321 acctctggat tacaaaattt gtgaaagatt