Construct: ORF TRCN0000488790
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022022.1_s317c1
- DNA Barcode:
- AATCACGACGAATAACTTTTGCGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC29A3 (55315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488790
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NM_018344.6 | 99.6% | 99.3% | (many diffs) |
| 2 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NM_001363518.1 | 83.2% | 82.9% | (many diffs) |
| 3 | human | 55315 | SLC29A3 | solute carrier family 29 me... | XM_017016377.2 | 68.9% | 68.6% | (many diffs) |
| 4 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NR_033413.1 | 58.7% | (many diffs) | |
| 5 | human | 55315 | SLC29A3 | solute carrier family 29 me... | XM_017016378.2 | 56.3% | 56.2% | (many diffs) |
| 6 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NM_001174098.1 | 54.1% | 53.8% | 473C>T;714_715delTGinsCA;774_775ins651 |
| 7 | human | 55315 | SLC29A3 | solute carrier family 29 me... | NR_033414.1 | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1494
- ORF length:
- 1425
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggccgttgtc tcagaggacg actttcagca cagttcaaac tccacctaca 121 gaaccacaag cagcagtctc cgagctgacc aggaggcact gcttgagaag ctgctggacc 181 gcccgccccc tggcctgcag aggcccgagg accgcttctg tggcacatac atcatcttct 241 tcagcctggg cattggcagt ctactgccat ggaacttctt tatcactgcc aaggagtact 301 ggatgttcaa actccgcaac tcctccagcc cagccaccgg ggaggaccct gagggctcag 361 acatcctgaa ctactttgag agctaccttg ccgttgcctc caccgtgccc tccatgctgt 421 gcctggtggc caacttcctg cttgtcaaca gggttgcagt ccacatccgt gtcctggcct 481 cactgacggt catcctggcc atcttcatgg tgataactgc actggtgaag gtggacactt 541 tctcctggac ccgtggcttt tttgcggtca ccattgtctg catggtgatc ctcagcggtg 601 cctccactgt cttcagcagc agcatctacg gcatgaccgg ctcctttcct atgaggaact 661 cccaggcact gatatcagga ggagccatgg gcgggacggt cagcgccgtg gcctcattgg 721 tggacttggc tgcatccagt gatgtgagga acagcgccct ggccttcttc ctgacggcca 781 ccatcttcct cgtgctctgc atgggactct acctgctgct gtccaggctg gagtatgcca 841 ggtactacat gaggcctgtt cttgcggccc atgtgttttc tggtgaagag gagcttcccc 901 aggactccct cagtgcccct tcggtggcct ccagattcat tgattcccac acaccccctc 961 tccgccccat cctgaagaag acggccagcc tgggcttctg tgtcacctac gtcttcttca 1021 tcaccagcct catctacccc gccgtctgca ccaacatcga gtccctcaac aagggctcgg 1081 gctcactgtg gaccaccaag tttttcatcc ccctcactac cttcctcctg tacaactttg 1141 ctgacctatg tggccggcag ctcaccgcct ggatccaggt gccagggccc aatagcaagg 1201 cgctcccagg gttcgtgctc ctccggacct gcctcatccc cctcttcgtg ctctgtaact 1261 accagccccg cgtccaccTG AAGACTGTGG TCTTCCAGTC CGATGTGTAC CCCGCACTCC 1321 TCAGCTCCCT GCTGGGGCTC AGCAACGGCT ACCTCAGCAC CCTGGCCCTC CTCTACGGGC 1381 CTAAGATTGT GCCCAGGGAG CTGGCTGAGG CCACGGGAGT GGTGATGTCC TTTTATGTGT 1441 GCTTGGGCTT AACACTGGGC TCAGCCTGCT CTACCCTCCT GGTGCACCTC ATCTAGGACC 1501 CAGCTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1561 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1621 ATATATCTTG TGGAAAGGAC GAAATCACGA CGAATAACTT TTGCGTACGC GTTAAGTCga 1681 caatcaacct ctggattaca aaatttgtga aagatt