Construct: ORF TRCN0000488809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019218.1_s317c1
- DNA Barcode:
- ATCATAATCGTTGACTTGATAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BDKRB2 (624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 624 | BDKRB2 | bradykinin receptor B2 | NM_000623.3 | 99.8% | 99.7% | 792A>G;1173_1174insG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1251
- ORF length:
- 1176
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgttc tctccctgga agatatcaat gtttctgtct gttcgtgagg 121 actccgtgcc caccacggcc tctttcagcg ccgacatgct caatgtcacc ttgcaagggc 181 ccactcttaa cgggaccttt gcccagagca aatgccccca agtggagtgg ctgggctggc 241 tcaacaccat ccagcccccc ttcctctggg tgctgttcgt gctggccacc ctagagaaca 301 tctttgtcct cagcgtcttc tgcctgcaca agagcagctg cacggtggca gagatctacc 361 tggggaacct ggccgcagca gacctgatcc tggcctgcgg gctgcccttc tgggccatca 421 ccatctccaa caacttcgac tggctctttg gggagacgct ctgccgcgtg gtgaatgcca 481 ttatctccat gaacctgtac agcagcatct gtttcctgat gctggtgagc atcgaccgct 541 acctggccct ggtgaaaacc atgtccatgg gccggatgcg cggcgtgcgc tgggccaagc 601 tctacagctt ggtgatctgg gggtgtacgc tgctcctgag ctcacccatg ctggtgttcc 661 ggaccatgaa ggagtacagc gatgagggcc acaacgtcac cgcttgtgtc atcagctacc 721 catccctcat ctgggaagtg ttcaccaaca tgctcctgaa tgtcgtgggc ttcctgctgc 781 ccctgagtgt catcaccttc tgcacgatgc agatcatgca ggtgctgcgg aacaacgaga 841 tgcagaagtt caaggagatc cagacggaga ggagggccac ggtgctagtc ctggttgtgc 901 tgctgctatt catcatctgc tggctgccct tccagatcag caccttcctG GATACGCTGC 961 ATCGCCTCGG CATCCTCTCC AGCTGCCAGG ACGAGCGCAT CATCGATGTA ATCACACAGA 1021 TCGCCTCCTT CATGGCCTAC AGCAACAGCT GCCTCAACCC ACTGGTGTAC GTGATCGTGG 1081 GCAAGCGCTT CCGAAAGAAG TCTTGGGAGG TGTACCAGGG AGTGTGCCAG AAAGGGGGCT 1141 GCAGGTCAGA ACCCATTCAG ATGGAGAACT CCATGGGCAC ACTGCGGACC TCCATCTCCG 1201 TGGAACGCCA GATTCACAAA CTGCAGGACT GGGCAGGGAG CAGACAGGAC CCAGCTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGAATCATAA TCGTTGACTT GATAACTACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt