Construct: ORF TRCN0000488822
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021879.1_s317c1
- DNA Barcode:
- TCGTACTTAATCTAGTTCGGGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- TPO (7173)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488822
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7173 | TPO | thyroid peroxidase | NM_000547.5 | 99.9% | 99.8% | 12C>G;208C>G |
| 2 | human | 7173 | TPO | thyroid peroxidase | NM_001206744.2 | 99.9% | 99.8% | 12C>G;208C>G |
| 3 | human | 7173 | TPO | thyroid peroxidase | XM_024453086.1 | 98.6% | 98.6% | 1_36del;48C>G;244C>G |
| 4 | human | 7173 | TPO | thyroid peroxidase | NM_175721.3 | 95.2% | 95.1% | 12C>G;208C>G;2384_2385ins132 |
| 5 | human | 7173 | TPO | thyroid peroxidase | XM_024453090.1 | 94% | 93.9% | (many diffs) |
| 6 | human | 7173 | TPO | thyroid peroxidase | NM_001206745.1 | 93.8% | 93.7% | 12C>G;208C>G;1595_1596ins171 |
| 7 | human | 7173 | TPO | thyroid peroxidase | NM_175719.3 | 93.8% | 93.7% | 12C>G;208C>G;1595_1596ins171 |
| 8 | human | 7173 | TPO | thyroid peroxidase | XM_024453091.1 | 92.6% | 92.5% | (many diffs) |
| 9 | human | 7173 | TPO | thyroid peroxidase | XM_011510380.3 | 90.3% | 91.7% | (many diffs) |
| 10 | human | 7173 | TPO | thyroid peroxidase | XM_024453087.1 | 89.7% | 88.4% | (many diffs) |
| 11 | human | 7173 | TPO | thyroid peroxidase | XM_024453088.1 | 89.7% | 88.4% | (many diffs) |
| 12 | human | 7173 | TPO | thyroid peroxidase | XM_024453089.1 | 89.7% | 88.4% | (many diffs) |
| 13 | human | 7173 | TPO | thyroid peroxidase | XM_024453085.1 | 88.7% | 87.3% | (many diffs) |
| 14 | human | 7173 | TPO | thyroid peroxidase | XM_024453092.1 | 87.9% | 87.9% | (many diffs) |
| 15 | human | 7173 | TPO | thyroid peroxidase | NM_175722.3 | 81.3% | 81.3% | 12C>G;208C>G;819_820ins519 |
| 16 | human | 7173 | TPO | thyroid peroxidase | XM_024453093.1 | 80.3% | 80.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2868
- ORF length:
- 2799
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gagagcgctg gctgtgctgt ctgtcacgct ggttatggcc tgcacagaag 121 ccttcttccc cttcatctcg agagggaaag aactcctttg gggaaagcct gaggagtctc 181 gtgtctctag cgtcttggag gaaagcaagc gcctggtgga caccgccatg tacgccacga 241 tgcagagaaa cctcaagaaa agaggaatcc tttctgcagc tcagcttctg tctttttcca 301 aacttcctga gccaacaagc ggagtgattg cccgagcagc agagataatg gaaacatcaa 361 tacaagcgat gaaaagaaaa gtcaacctga aaactcaaca atcacagcat ccaacggatg 421 ctttatcaga agatctgctg agcatcattg caaacatgtc tggatgtctc ccttacatgc 481 tgcccccaaa atgcccaaac acttgcctgg cgaacaaata caggcccatc acaggagctt 541 gcaacaacag agaccacccc agatggggcg cctccaacac ggccctggca cgatggctcc 601 ctccagtcta tgaggacggc ttcagtcagc cccgaggctg gaaccccggc ttcttgtaca 661 acgggttccc actgcccccg gtccgggagg tgacaagaca tgtcattcaa gtttcaaatg 721 aggttgtcac agatgatgac cgctattctg acctcctgat ggcatgggga caatacatcg 781 accacgacat cgcgttcaca ccacagagca ccagcaaagc tgccttcggg ggaggggctg 841 actgccagat gacttgtgag aaccaaaacc catgttttcc catacaactc ccggaggagg 901 cccggccggc cgcgggcacc gcctgtctgc ccttctaccg ctcttcggcc gcctgcggca 961 ccggggacca aggcgcgctc tttgggaacc tgtccacggc caacccgcgg cagcagatga 1021 acgggttgac ctcgttcctg gacgcgtcca ccgtgtatgg cagctccccg gccctagaga 1081 ggcagctgcg gaactggacc agtgccgaag ggctgctccg cgtccacgcg cgcctccggg 1141 actccggccg cgcctacctg cccttcgtgc cgccacgcgc gcctgcggcc tgtgcgcccg 1201 agcccggcat ccccggagag acccgcgggc cctgcttcct ggccggagac ggccgcgcca 1261 gcgaggtccc ctccctgacg gcactgcaca cgctgtggct gcgcgagcac aaccgcctgg 1321 ccgcggcgct caaggccctc aatgcgcact ggagcgcgga cgccgtgtac caggaggcgc 1381 gcaaggtcgt gggcgctctg caccagatca tcaccctgag ggattacatc cccaggatcc 1441 tgggacccga ggccttccag cagtacgtgg gtccctatga aggctatgac tccaccgcca 1501 accccactgt gtccaacgtg ttctccacag ccgccttccg cttcggccat gccacgatcc 1561 acccgctggt gaggaggctg gacgccagct tccaggagca ccccgacctg cccgggctgt 1621 ggctgcacca ggctttcttc agcccatgga cattactccg tggaggtggt ttggacccac 1681 taatacgagg ccttcttgca agaccagcca aactgcaggt gcaggatcag ctgatgaacg 1741 aggagctgac ggaaaggctc tttgtgctgt ccaattccag caccttggat ctggcgtcca 1801 tcaacctgca gaggggccgg gaccacgggc tgccaggtta caatgagtgg agggagttct 1861 gcggcctgcc tcgcctggag acccccgctg acctgagcac agccatcgcc agcaggagcg 1921 tggccgacaa gatcctggac ttgtacaagc atcctgacaa catcgatgtc tggctgggag 1981 gcttagctga aaacttcctc cccagggctc ggacagggcc cctgtttgcc tgtctcattg 2041 ggaagcagat gaaggctctg cgggacggtg actggttttg gtgggagaac agccacgtct 2101 tcacggatgc acagaggcgt gagctggaga agcactccct gtctcgggtc atctgtgaca 2161 acactggcct caccagggtg cccatggatg ccttccaagt cggcaaattc cccgaagact 2221 ttgagtcttg tgacagcatc actggcatga acctggaggc ctggagggaa acctttcctc 2281 aagacgacaa gtgtggcttc ccagagagcg tggagaatgg ggactttgtg cactgtgagg 2341 agtctgggag gcgcgtgctg gtgtattcct gccggcacgg gtatgagctc caaggccggg 2401 agcagctcac ttgcacccag gaaggatggg atttccagcc tcccctctgc aaagatgtga 2461 acgagtgtgc agacggtgcc caccccccct gccacgcctc tgcgaggtgc agaaacacca 2521 aaggcggctt ccagtgtctc tgcgcggacc cctacgagtt aggagacgat gggagaaccT 2581 GCGTAGACTC CGGGAGGCTC CCTCGGGTGA CTTGGATCTC CATGTCGCTG GCTGCTCTGC 2641 TGATCGGAGG CTTCGCAGGT CTCACCTCGA CGGTGATTTG CAGGTGGACA CGCACTGGCA 2701 CTAAATCCAC ACTGCCCATC TCGGAGACAG GCGGAGGAAC TCCCGAGCTG AGATGCGGAA 2761 AGCACCAGGC CGTAGGGACC TCACCGCAGC GGGCCGCAGC TCAGGACTCG GAGCAGGAGA 2821 GTGCTGGGAT GGAAGGCCGG GATACTCACA GGCTGCCGAG AGCCCTCTAG AACCCAGCTT 2881 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2941 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3001 CTTGTGGAAA GGACGATCGT ACTTAATCTA GTTCGGGTTA ACGCGTTAAG TCgacaatca 3061 acctctggat tacaaaattt gtgaaagatt