Construct: ORF TRCN0000488823
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021885.1_s317c1
- DNA Barcode:
- GACCTTTCTCCAAAGGCAAACTAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRA1 (84435)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488823
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | NM_001083909.2 | 43.6% | 43.5% | 0_1ins2151;2_3insCCCGACACA;498_500delGTA |
| 2 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | NM_001291085.1 | 36% | 36% | 0_1ins2451;207_209delGTA |
| 3 | human | 84435 | ADGRA1 | adhesion G protein-coupled ... | XM_011540273.1 | 30.5% | 30.5% | 0_1ins2664 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3906
- ORF length:
- 3837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gcgaggccac gggaaccact cgcggctgaa atccagatgt gcttctctgg 121 gcaacgtcca tcaccaccag gcacagatgg cgcacggtgc acactgccga gggggccgga 181 caccacagtc accagacagc cggggcccaa ccgctggggg ccacacccgg ggcagatgga 241 cgtaccggcc catcgaggcc ctcatcaacg tcagctttcc cggagcaaag ggtgggagaa 301 cggccgaggg cgagccctca tgggcgtggg tcttcagaag tggctatttg cagagacagg 361 ctcttcctgg gcttcccgtc tccacccgag tggctgcctg ctggtggcaa caccatccag 421 gcagggactc agggttcttg ggacctggat ttattgacct gctcagagag gagcccagcg 481 gccgtgcaga tgggaaggca gctgctcaga aaacagggcg tctttatttg ttcccgactt 541 tcctaggcaa caaattacca gaggccgccg actcgtttgg ggaaattgct tctgggaaca 601 gtgttatttt tacctccgtc ttgcaatcct ctcacccacc tggcacccca ccgcccagcg 661 cacgcaagcc aagtcccgga gctgccatgc tcgtctgtga ggaaacgctg gagctgggtg 721 cccagggctg ctgggggtcc acgagccccc ggggccaggc cgtgagcttg gtcggtgggc 781 ccacggccgg aggctccagg ctgggagggg ctggctgcac catgggcctg ctcatggccc 841 tccaggccag acgccccagg gacggcacct gggctgaccc tcgaagatgc tccagggctg 901 tggagggctc gggcctcacc agggctgccc ttgtgagtcc agggccatcg ggggtggagg 961 aggacaccag gagggaaggc tgcgtgcttg tgggctcctg gaggtcgtcc acccacagaa 1021 gccgtgttct gattatcaac acgccctctg gcaggacccc ctggagccag ggctccctcg 1081 gcagcaccgg gcgctacaac cagacccggg caccaggcag ccagcagcag gggctgtgct 1141 cggatgcagc tctacacgag gattttgtgc cggagcctac tcctgccttc tttcaatacc 1201 ctccacaagc cttcagcaaa accaaggttc gcgacttggg gaggggaggc ctttatgcct 1261 tttccaagga ggagaaggta tctgaggccc agcgggtaga ggcctacgag acccacggcc 1321 ttggcctgag tacaggccga cagaaccctg tgccgtcccc ctgtaagcct ggatgcgtgt 1381 gccagggcct tgcgctggac gcagaggcct ggccgaggca ccctggacgc aggccctgtg 1441 cggccaagga ggcctgcctg ggcccacctg gctgcagcgg ggagctgagc ctccctgagg 1501 aggaaggaat cgcacctggg gcagcaacag cagctcctgc ctgggtccct catggccgcg 1561 tgagccgctt cctcactgta aagctcttca cctatgtaac tatgtatcaa tgtaaacagg 1621 aacgtatcct ccgcctctgc atctctggtg aatccgggcg atacgtgcgg cgctcacgct 1681 tccgcaactt tgcagcgctc atggtgagta cgggggtccc gggggtcctg cagctggggg 1741 ctaggccgct gcgggggggc ggccacccgt atggggactc ccaggctgga aaagttgtgg 1801 aatggcgagc tggtgaccgg gcttccgggc tcagtgccgg ggaaagaagg ttcctcagcc 1861 agtcggagct gcagacctgg ccccggacac ctcactgtgt aggaaagaag gttcctcagc 1921 cagttggagc tgcagaccta gccccggaca cctcactgtg caggaaagaa ggttcctcag 1981 ccagtcggag ctgcagacct ggccccggac acctcacccg cagggaggaa gattccttca 2041 ggcagctggc attccccagg tttgaagagg tcactttccc acctctcact gctgcagaaa 2101 gagcaccctc tgtccacggt ggacactctc tagccagcca gggcctcgtc ttggctggac 2161 ggacaagaag tgtggcctca ggagggaagc aggagctctc gggcccgctg gctgcctgca 2221 tcccgacaca ggatctgaag acagtgctct ccctgccccg ctacccaggg gagttcctgc 2281 accccgtggt gtacgcgtgc acggccgtca tgctgctctg cctcctggcc tccttcgtca 2341 cctacatcgt gcaccagagc gccatccgca tcagccgcaa gggccggcac acgctcctga 2401 atttctgctt ccacgcggcc ctgaccttca ctgtgttcgc cggcggcatc aatcgcacca 2461 agtaccccat cctgtgccag gcggtgggca tcgtgctgca ctattctaca ctgtccacca 2521 tgctgtggat aggagtgacc gccaggaaca tctacaagca ggtgaccaag aaggcccctc 2581 tgtgcctgga cacagaccag ccaccgtacc ccaggcagcc cctgctcagg ttttacctcg 2641 tcagcggagg ggtccccttt atcatctgtg gggtcacggc tgccacgaac atcaggaatt 2701 acgggacaga ggacgaggac acggcctgct ggatggcctg ggagcccagc ctgggcgcct 2761 tctacggccc agccgccatc atcaccctgg tcacctgtgt gtacttcctg ggcacctacg 2821 tgcagctgcg gcgccaccca gggcgcaggt acgagctgcg cacacagccc gaggagcagc 2881 ggcggctggc gacacccgag ggcggccgtg ggatccggcc aggcacccca cccgcacacg 2941 atgcccccgg cgcctccgtg ctgcagaacg agcactcatt ccaggcacag ctgcgcgccg 3001 ccgccttcac gctgttcctg ttcacggcca cgtgggcctt cggggcgctg gcggtgtcac 3061 agggccactt cctggacatg gtcttcagct gcctgtacgg cgccttctgc gtgaccctgg 3121 gactcttcgt gctcatccac cactgcgcca agcgtgagga cgtgtggcag tgctggtggg 3181 catgctgccc gccccgcaag gacgcccacc ccgcacttga cgccaacggg gccgcgctgg 3241 gccgcgccgc ctgcctgcac tcgccgggac tgggccagcc acggggcttc gcgcacccac 3301 cgggcccctg caagatgacc aacctgcagg ccgcgcaggg ccacgccagt tgcctgtcac 3361 cggccacccc gtgctgcgcc aagatgcact gcgagccact gacggcggac gaggcgcacg 3421 tgcacctgca ggaggagggc gccttcgggc acgaccccca cctgcacggg tgccttcagg 3481 gcagaactaa gccgccctac tttagccggc acccagcaga ggagcccgag tacgcctacc 3541 acatcccatc cagcctggat ggcagccccc gcagctcgcg cacagacagc ccccccagct 3601 ctctggatgg cccGGCGGGG ACACACACGC TGGCCTGCTG CACCCAGGGC GACCCCTTCC 3661 CCATGGTCAC CCAGCCCGAG GGCAGTGATG GGAGCCCTGC CCTCTACAGC TGCCCCACGC 3721 AGCCGGGCAG GGAGGCAGCG CTCGGGCCCG GCCACTTGGA GATGCTGCGG AGGACACAGT 3781 CCCTGCCCTT TGGTGGCCCC AGCCAGAACG GGCTGCCCAA GGGTAAATTG CTAGAAGGCC 3841 TGCCGTTTGG CACCGACGGG ACCGGCAACA TCCGAACGGG ACCCTGGAAA AACGAAACTA 3901 CTGTGTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 3961 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 4021 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACCTT TCTCCAAAGG CAAACTAAAC 4081 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt