Construct: ORF TRCN0000488833
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021072.1_s317c1
- DNA Barcode:
- CCCATTATCTCAGCGGCCTCCTCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GRM6 (2916)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488833
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2916 | GRM6 | glutamate metabotropic rece... | NM_000843.4 | 100% | 100% | |
| 2 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | NM_173372.2 | 87.2% | 93.2% | (many diffs) |
| 3 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | XM_006531961.2 | 87.2% | 93.2% | (many diffs) |
| 4 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | XM_017314218.1 | 52.3% | 56.6% | (many diffs) |
| 5 | mouse | 108072 | Grm6 | glutamate receptor, metabot... | XM_006531964.1 | 48.8% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2703
- ORF length:
- 2631
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcgcgg ccccggagag cccgggagcc gctgctcgtg gcgctgctgc 121 cgctggcgtg gctggcgcag gcgggcctgg cgcgcgcggc gggctctgtg cgcctggcgg 181 gcggcctgac gctgggcggc ctgttcccgg tgcacgcgcg gggcgcggcg ggccgggcgt 241 gcgggcagct gaagaaggag cagggcgtgc accggctgga ggccatgctg tacgcgctgg 301 accgcgtcaa cgccgacccc gagctgctgc ccggcgtgcg cctgggcgcg cggctgctgg 361 acacctgctc gcgggacacc tacgcgctgg agcaggcgct gagcttcgtg caggcgctga 421 tccgcggccg cggcgacggc gacgaggtgg gcgtgcgctg cccgggaggc gtccctccgc 481 tgcgccccgc gccccccgag cgcgtcgtgg ccgtcgtggg cgcctcggcc agctccgtct 541 ccatcatggt cgccaacgtg ctgcgcctgt ttgcgatacc ccagatcagc tatgcctcca 601 cagccccgga gctcagcgac tccacacgct atgacttctt ctcccgggtg gtgccacccg 661 actcctacca ggcgcaggcc atggtggaca tcgtgagggc actgggatgg aactatgtgt 721 ccacgctggc ctccgagggc aactatggcg aaagtggggt tgaggccttc gttcagatct 781 cccgagaggc tgggggggtc tgtattgccc agtctatcaa gattcccagg gaaccaaagc 841 caggagagtt cagcaaggtg atcaggagac tcatggagac gcccaacgcc cggggcatca 901 tcatctttgc caatgaggat gacatcaggc gggtcctgga ggcagctcgc caggccaacc 961 tgaccggcca cttcctgtgg gtcggctcag acagctgggg agccaagacc tcacccatct 1021 tgagcctgga ggacgtggcc gttggggcca tcaccatcct gcccaaaagg gcctccatcg 1081 acggatttga ccagtacttc atgactcgat ccctggagaa caaccgcagg aacatctggt 1141 tcgccgagtt ctgggaagag aattttaact gcaaactgac cagctcaggt acccagtcag 1201 acgattccac ccgcaaatgc acaggcgagg aacgcatcgg ccgggactcc acctacgagc 1261 aggagggcaa ggtgcagttt gtgattgatg cggtgtacgc cattgcccac gccctccaca 1321 gcatgcacca ggcgctctgc cctgggcaca caggcctgtg cccggcgatg gaacccactg 1381 atgggcggat gcttctgcag tacattcgag ctgtccgctt caatggcagc gcaggaaccc 1441 ctgtgatgtt caacgagaac ggagatgcgc ccgggcggta cgacatcttc cagtaccagg 1501 cgaccaatgg cagtgccagc agtggcgggt accaggcagt gggccagtgg gcagagaccc 1561 tcagactgga tgtggaggcc ctgcagtggt ctggcgaccc ccacgaggtg ccctcgtctc 1621 tgtgcagcct gccctgcggg ccgggggagc ggaagaagat ggtgaagggc gtcccctgct 1681 gttggcactg cgaggcctgt gacgggtacc gcttccaggt ggacgagttc acatgcgagg 1741 cctgtcctgg ggacatgagg cccacgccca accacacggg ctgccgcccc acacctgtgg 1801 tgcgcctgag ctggtcctcc ccctgggcag ccccgccgct cctcctggcc gtgctgggca 1861 tcgtggccac taccacggtg gtggccacct tcgtgcggta caacaacacg cccatcgtcc 1921 gggcctcggg ccgagagctc agctacgtcc tcctcaccgg catcttcctc atctacgcca 1981 tcaccttcct catggtggct gagcctgggg ccgcggtctg tgccgcccgc aggctcttcc 2041 tgggcctggg cacgaccctc agctactctg ccctgctcac caagaccaac cgtatctacc 2101 gcatctttga gcagggcaag cgctcggtca caccccctcc cttcatcagc cccacctcac 2161 agctggtcat caccttcagc ctcacctccc tgcaggtggt ggggatgata gcatggctgg 2221 gggcccggcc cccacacagc gtgattgact atgaggaaca gcggacggtg gaccccgagc 2281 aggccagagg ggtgctcaag tgcgacatgt cggatctgtc tctcatcggc tgcctgggct 2341 acagcctcct gctcatggtc acgtgcacag tgtacgccat caaggcccgt ggcgtgcccg 2401 agaccttcaa cgaggccaag cccatcggct tcaccatgta caccaccTGC ATCATCTGGC 2461 TGGCATTCGT GCCCATCTTC TTTGGCACTG CCCAGTCAGC TGAAAAGATC TACATCCAGA 2521 CAACCACGCT AACCGTGTCC TTGAGCCTGA GTGCCTCGGT GTCCCTCGGC ATGCTCTACG 2581 TACCCAAAAC CTACGTCATC CTCTTCCATC CAGAGCAGAA TGTGCAGAAG CGAAAGCGGA 2641 GCCTCAAGGC CACCTCCACG GTGGCAGCCC CACCCAAGGG CGAGGATGCA GAGGCCCACA 2701 AGTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2761 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2821 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCATTATC TCAGCGGCCT CCTCGACGCG 2881 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt