Construct: ORF TRCN0000488833

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021072.1_s317c1
DNA Barcode:
CCCATTATCTCAGCGGCCTCCTCG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
GRM6 (2916)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000488833
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2916 GRM6 glutamate metabotropic rece... NM_000843.4 100% 100%
2 mouse 108072 Grm6 glutamate receptor, metabot... NM_173372.2 87.2% 93.2% (many diffs)
3 mouse 108072 Grm6 glutamate receptor, metabot... XM_006531961.2 87.2% 93.2% (many diffs)
4 mouse 108072 Grm6 glutamate receptor, metabot... XM_017314218.1 52.3% 56.6% (many diffs)
5 mouse 108072 Grm6 glutamate receptor, metabot... XM_006531964.1 48.8% 53% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2703
ORF length:
2631
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcgcgg ccccggagag cccgggagcc gctgctcgtg gcgctgctgc
121cgctggcgtg gctggcgcag gcgggcctgg cgcgcgcggc gggctctgtg cgcctggcgg
181gcggcctgac gctgggcggc ctgttcccgg tgcacgcgcg gggcgcggcg ggccgggcgt
241gcgggcagct gaagaaggag cagggcgtgc accggctgga ggccatgctg tacgcgctgg
301accgcgtcaa cgccgacccc gagctgctgc ccggcgtgcg cctgggcgcg cggctgctgg
361acacctgctc gcgggacacc tacgcgctgg agcaggcgct gagcttcgtg caggcgctga
421tccgcggccg cggcgacggc gacgaggtgg gcgtgcgctg cccgggaggc gtccctccgc
481tgcgccccgc gccccccgag cgcgtcgtgg ccgtcgtggg cgcctcggcc agctccgtct
541ccatcatggt cgccaacgtg ctgcgcctgt ttgcgatacc ccagatcagc tatgcctcca
601cagccccgga gctcagcgac tccacacgct atgacttctt ctcccgggtg gtgccacccg
661actcctacca ggcgcaggcc atggtggaca tcgtgagggc actgggatgg aactatgtgt
721ccacgctggc ctccgagggc aactatggcg aaagtggggt tgaggccttc gttcagatct
781cccgagaggc tgggggggtc tgtattgccc agtctatcaa gattcccagg gaaccaaagc
841caggagagtt cagcaaggtg atcaggagac tcatggagac gcccaacgcc cggggcatca
901tcatctttgc caatgaggat gacatcaggc gggtcctgga ggcagctcgc caggccaacc
961tgaccggcca cttcctgtgg gtcggctcag acagctgggg agccaagacc tcacccatct
1021tgagcctgga ggacgtggcc gttggggcca tcaccatcct gcccaaaagg gcctccatcg
1081acggatttga ccagtacttc atgactcgat ccctggagaa caaccgcagg aacatctggt
1141tcgccgagtt ctgggaagag aattttaact gcaaactgac cagctcaggt acccagtcag
1201acgattccac ccgcaaatgc acaggcgagg aacgcatcgg ccgggactcc acctacgagc
1261aggagggcaa ggtgcagttt gtgattgatg cggtgtacgc cattgcccac gccctccaca
1321gcatgcacca ggcgctctgc cctgggcaca caggcctgtg cccggcgatg gaacccactg
1381atgggcggat gcttctgcag tacattcgag ctgtccgctt caatggcagc gcaggaaccc
1441ctgtgatgtt caacgagaac ggagatgcgc ccgggcggta cgacatcttc cagtaccagg
1501cgaccaatgg cagtgccagc agtggcgggt accaggcagt gggccagtgg gcagagaccc
1561tcagactgga tgtggaggcc ctgcagtggt ctggcgaccc ccacgaggtg ccctcgtctc
1621tgtgcagcct gccctgcggg ccgggggagc ggaagaagat ggtgaagggc gtcccctgct
1681gttggcactg cgaggcctgt gacgggtacc gcttccaggt ggacgagttc acatgcgagg
1741cctgtcctgg ggacatgagg cccacgccca accacacggg ctgccgcccc acacctgtgg
1801tgcgcctgag ctggtcctcc ccctgggcag ccccgccgct cctcctggcc gtgctgggca
1861tcgtggccac taccacggtg gtggccacct tcgtgcggta caacaacacg cccatcgtcc
1921gggcctcggg ccgagagctc agctacgtcc tcctcaccgg catcttcctc atctacgcca
1981tcaccttcct catggtggct gagcctgggg ccgcggtctg tgccgcccgc aggctcttcc
2041tgggcctggg cacgaccctc agctactctg ccctgctcac caagaccaac cgtatctacc
2101gcatctttga gcagggcaag cgctcggtca caccccctcc cttcatcagc cccacctcac
2161agctggtcat caccttcagc ctcacctccc tgcaggtggt ggggatgata gcatggctgg
2221gggcccggcc cccacacagc gtgattgact atgaggaaca gcggacggtg gaccccgagc
2281aggccagagg ggtgctcaag tgcgacatgt cggatctgtc tctcatcggc tgcctgggct
2341acagcctcct gctcatggtc acgtgcacag tgtacgccat caaggcccgt ggcgtgcccg
2401agaccttcaa cgaggccaag cccatcggct tcaccatgta caccaccTGC ATCATCTGGC
2461TGGCATTCGT GCCCATCTTC TTTGGCACTG CCCAGTCAGC TGAAAAGATC TACATCCAGA
2521CAACCACGCT AACCGTGTCC TTGAGCCTGA GTGCCTCGGT GTCCCTCGGC ATGCTCTACG
2581TACCCAAAAC CTACGTCATC CTCTTCCATC CAGAGCAGAA TGTGCAGAAG CGAAAGCGGA
2641GCCTCAAGGC CACCTCCACG GTGGCAGCCC CACCCAAGGG CGAGGATGCA GAGGCCCACA
2701AGTAGGACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2761TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2821CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCATTATC TCAGCGGCCT CCTCGACGCG
2881TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)

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