Construct: ORF TRCN0000488835
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021041.1_s317c1
- DNA Barcode:
- GTACGCTATTTAGTCTCCATTACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- OGFR (11054)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488835
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11054 | OGFR | opioid growth factor receptor | NM_007346.4 | 96.9% | 97% | 1639_1698del;1818G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2043
- ORF length:
- 1971
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggacgac cccgactgcg actccacctg ggaggaggac gaggaggatg 121 cggaggacgc ggaggacgag gactgcgagg acggcgaggc cgccggcgcg agggacgcgg 181 acgcagggga cgaggacgag gagtcggagg agccgcgggc ggcgcggccc agctcgttcc 241 agtccagaat gacagggtcc agaaactggc gagccacgag ggacatgtgt aggtatcggc 301 acaactatcc ggatctggtg gaacgagact gcaatgggga cacgccaaac ctgagtttct 361 acagaaatga gatccgcttc ctgcccaacg gctgtttcat tgaggacatt cttcagaact 421 ggacggacaa ctatgacctc cttgaggaca atcactccta catccagtgg ctgtttcctc 481 tgcgagaacc aggagtgaac tggcatgcca agcccctcac gctcagggag gtcgaggtgt 541 ttaaaagctc ccaggagatc caggagcggc ttgtccgggc ctacgagctc atgctgggct 601 tctacgggat ccggctggag gaccgaggca cgggcacggt gggccgagca cagaactacc 661 agaagcgctt ccagaacctg aactggcgca gccacaacaa cctccgcatc acacgcatcc 721 tcaagtcgct gggtgagctg ggcctcgagc acttccaggc gccgctggtc cgcttcttcc 781 tggaggagac gctggtgcgg cgggagctgc cgggggtgcg gcagagtgcc ctggactact 841 tcatgttcgc cgtgcgctgc cgacaccagc gccgccagct ggtgcacttc gcctgggagc 901 acttccggcc ccgctgcaag ttcgtctggg ggccccaaga caagctgcgg aggttcaagc 961 ccagctctct gccccatccg ctcgagggct ccaggaaggt ggaggaggaa ggaagccccg 1021 gggaccccga ccacgaggcc agcacccagg gtcggacctg tgggccagag catagcaagg 1081 gtgggggcag ggtggacgag gggccccagc cacggagcgt ggagccccag gatgcgggac 1141 ccctggagag gagccagggg gatgaggcag ggggccacgg ggaagatagg ccggagccct 1201 taagccccaa agagagcaag aagaggaagc tggagctgag ccggcgggag cagccgccca 1261 cagagccagg ccctcagagt gcctcagagg tggagaagat cgctctgaat ttggaggggt 1321 gtgccctcag ccagggcagc ctcaggacgg ggacccagga agtgggcggt caggaccctg 1381 gggaggcagt gcagccctgc cgccaacccc tgggagccag ggtggccgac aaggtgagga 1441 agcggaggaa ggtggatgag ggtgctgggg acagtgctgc ggtggccagt ggtggtgccc 1501 agaccttggc ccttgccggg tcccctgccc catcggggca ccccaaggct ggacacagtg 1561 agaacggggt tgaggaggac acagaaggtc gaacggggcc caaagaaggt acccctggga 1621 gcccatcgga gaccccaggc cccagcccag caggaccTGC AGGGGACGAG CCAGCCGAGA 1681 GCCCATCGGA GACCCCAGGC CCCCGCCCAG CAGGACCTGC AGGGGACGAG CCAGCCGAGA 1741 GCCCATCGGA GACCCCAGGC CCCAGCCCGG CAGGACCTAC AAGGGATGAG CCAGCCGAGA 1801 GCCCATCGGA GACCCCAGGC CCCCGCCCAG CAGGACCTGC AGGGGACGAG CCAGCCGAGA 1861 GCCCATCGGA GACCCCAGGC CCCCGCCCGG CAGGACCTGC AGGGGACGAG CCAGCCGAGA 1921 GCCCATCGGA GACCCCAGGC CCCAGCCCGG CAGGACCTAC AAGGGATGAG CCAGCCAAGG 1981 CGGGGGAGGC AGCAGAGTTG CAGGACGCAG AGGTGGAGTC TTCTGCCAAG TCTGGGAAGC 2041 CTTAGAACCC AGCTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2101 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2161 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTACGCTAT TTAGTCTCCA TTACAACGCG 2221 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt