Construct: ORF TRCN0000488842
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020403.1_s317c1
- DNA Barcode:
- GCATTGGCATGCGCTGTCGGGTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- DAP (1611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488842
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1611 | DAP | death associated protein | NM_004394.3 | 100% | 100% | |
| 2 | human | 1611 | DAP | death associated protein | NM_001291963.2 | 40.1% | 27.5% | 151_152ins43;264_612del |
| 3 | mouse | 223453 | Dap | death-associated protein | NM_146057.3 | 86.6% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 378
- ORF length:
- 306
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgtcttcg ccTCCCGAAG GGAAACTAGA GACTAAAGCT GGACACCCGC 121 CCGCCGTGAA AGCTGGTGGA ATGCGAATTG TGCAGAAACA CCCACATACA GGAGACACCA 181 AAGAAGAGAA AGACAAGGAT GACCAGGAAT GGGAAAGCCC CAGTCCACCT AAACCCACTG 241 TGTTCATCTC TGGGGTCATC GCCCGGGGTG ACAAAGATTT CCCCCCGGCG GCTGCGCAGG 301 TGGCTCACCA GAAGCCGCAT GCCTCCATGG ACAAGCATCC TTCCCCAAGA ACCCAGCACA 361 TCCAGCAGCC ACGCAAGTAG GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAT TGGCATGCGC 541 TGTCGGGTTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt