Construct: ORF TRCN0000488855
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021877.1_s317c1
- DNA Barcode:
- GGCCCCACAATGGGCACACGACAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GNA15 (2769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488855
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2769 | GNA15 | G protein subunit alpha 15 | NM_002068.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1191
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggcccgctcg ctgacctggc gctgctgccc ctggtgcctg acggaggatg 121 agaaggccgc cgcccgggtg gaccaggaga tcaacaggat cctcttggag cagaagaagc 181 aggaccgcgg ggagctgaag ctgctgcttt tgggcccagg cgagagcggg aagagcacct 241 tcatcaagca gatgcggatc atccacggcg ccggctactc ggaggaggag cgcaagggct 301 tccggcccct ggtctaccag aacatcttcg tgtccatgcg ggccatgatc gaggccatgg 361 agcggctgca gattccattc agcaggcccg agagcaagca ccacgctagc ctggtcatga 421 gccaggaccc ctataaagtg accacgtttg agaagcgcta cgctgcggcc atgcagtggc 481 tgtggaggga tgccggcatc cgggcctgct atgagcgtcg gcgggaattc cacctgctcg 541 attcagccgt gtactacctg tcccacctgg agcgcatcac cgaggagggc tacgtcccca 601 cagctcagga cgtgctccgc agccgcatgc ccaccactgg catcaacgag tactgcttct 661 ccgtgcagaa aaccaacctg cggatcgtgg acgtcggggg ccagaagtca gagcgtaaga 721 aatggatcca ttgtttcgag aacgtgatcg ccctcatcta cctggcctca ctgagtgaat 781 acgaccagtg cctggaggag aacaaccagg agaaccgcat gaaggagagc ctcgcattgt 841 ttgggactat cctggaacta cccTGGTTCA AAAGCACATC CGTCATCCTC TTTCTCAACA 901 AAACCGACAT CCTGGAGGAG AAAATCCCCA CCTCCCACCT GGCTACCTAT TTCCCCAGTT 961 TCCAGGGCCC TAAGCAGGAT GCTGAGGCAG CCAAGAGGTT CATCCTGGAC ATGTACACGA 1021 GGATGTACAC CGGGTGCGTG GACGGCCCCG AGGGCAGCAA GAAGGGCGCA CGATCCCGAC 1081 GCCTCTTCAG CCACTACACA TGTGCCACAG ACACACAGAA CATCCGCAAG GTCTTCAAGG 1141 ACGTGCGGGA CTCGGTGCTC GCCCGCTACC TGGACGAGAT CAACCTGCTG TAGAACCCAG 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAG GCCCCACAAT GGGCACACGA CAAACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att