Construct: ORF TRCN0000488860
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021043.1_s317c1
- DNA Barcode:
- TTCTCAAGTCCTAGAACCACTGGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- P2RY8 (286530)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488860
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286530 | P2RY8 | P2Y receptor family member 8 | NM_178129.5 | 100% | 100% | |
| 2 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_005274429.3 | 100% | 100% | |
| 3 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_005274778.3 | 100% | 100% | |
| 4 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_011545631.2 | 100% | 100% | |
| 5 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_011545632.2 | 100% | 100% | |
| 6 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_011546178.2 | 100% | 100% | |
| 7 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_011546179.2 | 100% | 100% | |
| 8 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_006724443.3 | 65.5% | 65.5% | 1_567del |
| 9 | human | 286530 | P2RY8 | P2Y receptor family member 8 | XM_006724864.3 | 65.5% | 65.5% | 1_567del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1149
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcaggtc ccgaacagca ccggcccgga caacgcgacg ctgcagatgc 121 tgcggaaccc ggcgatcgcg gtggccctgc ccgtggtgta ctcgctggtg gcggcggtca 181 gcatcccggg caacctcttc tctctgtggg tgctgtgccg gcgcatgggg cccagatccc 241 cgtcggtcat cttcatgatc aacctgagcg tcacggacct gatgctggcc agcgtgttgc 301 ctttccaaat ctactaccat tgcaaccgcc accactgggt attcggggtg ctgctttgca 361 acgtggtgac cgtggccttt tacgcaaaca tgtattccag catcctcacc atgacctgta 421 tcagcgtgga gcgcttcctg ggggtcctgt acccgctcag ctccaagcgc tggcgccgcc 481 gtcgttacgc ggtggccgcg tgtgcaggga cctggctgct gctcctgacc gccctgtccc 541 cgctggcgcg caccgatctc acctacccgg tgcacgccct gggcatcatc acctgcttcg 601 acgtcctcaa gtggacgatg ctccccagcg tggccatgtg ggccgtgttc ctcttcacca 661 tcttcatcct gctgttcctc atcccgttcg tgatcaccgt ggcttgttac acggccacca 721 tcctcaagct gttgcgcacg gaggaggcgc acggccggga gcagcggagg cgcgcggtgg 781 gcctggccgc ggtggtcttg ctggcctttg tcaccTGCTT CGCCCCCAAC AACTTCGTGC 841 TCCTGGCGCA CATCGTGAGC CGCCTGTTCT ACGGCAAGAG CTACTACCAC GTGTACAAGC 901 TCACGCTGTG TCTCAGCTGC CTCAACAACT GTCTGGACCC GTTTGTTTAT TACTTTGCGT 961 CCCGGGAATT CCAGCTGCGC CTGCGGGAAT ATTTGGGCTG CCGCCGGGTG CCCAGAGACA 1021 CCCTGGACAC GCGCCGCGAG AGCCTCTTCT CCGCCAGGAC CACGTCCGTG CGCTCCGAGG 1081 CCGGTGCGCA CCCTGAAGGG ATGGAGGGAG CCACCAGGCC CGGCCTCCAG AGGCAGGAGA 1141 GTGTGTTCTA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1201 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1261 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTC TCAAGTCCTA GAACCACTGG 1321 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t