Transcript: Human XM_006724443.3

PREDICTED: Homo sapiens P2Y receptor family member 8 (P2RY8), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
P2RY8 (286530)
Length:
4744
CDS:
195..1841

Additional Resources:

NCBI RefSeq record:
XM_006724443.3
NBCI Gene record:
P2RY8 (286530)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_006724443.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358415 CATACCCGAGGGTGGATATTC pLKO_005 2223 3UTR 100% 13.200 18.480 N P2RY8 n/a
2 TRCN0000014427 CGTGTTGCCTTTCCAAATCTA pLKO.1 983 CDS 100% 5.625 7.875 N P2RY8 n/a
3 TRCN0000014426 CAAGAGCTACTACCACGTGTA pLKO.1 1565 CDS 100% 4.050 5.670 N P2RY8 n/a
4 TRCN0000014423 GCTGGATTATTAAGCACCCTA pLKO.1 3771 3UTR 100% 2.640 2.112 N P2RY8 n/a
5 TRCN0000358413 AGCGCACAGGGTGCTTGTTAT pLKO_005 2071 3UTR 100% 13.200 9.240 N P2RY8 n/a
6 TRCN0000358330 ATCCTCACCATGACCTGTATC pLKO_005 1092 CDS 100% 10.800 7.560 N P2RY8 n/a
7 TRCN0000358331 ACAACTGTCTGGACCCGTTTG pLKO_005 1615 CDS 100% 6.000 4.200 N P2RY8 n/a
8 TRCN0000014424 CCTTTCCAAATCTACTACCAT pLKO.1 990 CDS 100% 3.000 2.100 N P2RY8 n/a
9 TRCN0000014425 CGCAAACATGTATTCCAGCAT pLKO.1 1073 CDS 100% 2.640 1.848 N P2RY8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006724443.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05417 pDONR223 100% 65.5% 65.5% None 1_567del n/a
2 ccsbBroad304_05417 pLX_304 51.3% 65.5% 65.5% V5 1_567del n/a
3 TRCN0000472077 TGGTCATGTGGGCGGGGAACGCCT pLX_317 36.5% 65.5% 65.5% V5 1_567del n/a
4 TRCN0000488860 TTCTCAAGTCCTAGAACCACTGGG pLX_317 31.3% 65.5% 65.5% V5 (not translated due to prior stop codon) 1_567del n/a
5 TRCN0000488418 TGTGAGACGCGCTCCGGGTCGATA pLX_317 31.1% 65.4% 65.3% V5 1_567del;1644_1645insG n/a
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