Construct: ORF TRCN0000488867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020219.1_s317c1
- DNA Barcode:
- ACCTGCAACGGTCGGCACTGAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CKM (1158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488867
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1158 | CKM | creatine kinase, M-type | NM_001824.5 | 99.7% | 99.7% | 369T>C;673T>C;1119T>C |
| 2 | mouse | 12715 | Ckm | creatine kinase, muscle | NM_007710.2 | 90.3% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1215
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgccattc ggtaacaccc acaacaagtt caagctgaat tacaagcctg 121 aggaggagta ccccgacctc agcaaacata acaaccacat ggccaaggta ctgacccttg 181 aactctacaa gaagctgcgg gacaaggaga ctccatctgg cttcactgta gacgatgtca 241 tccagacagg agtggacaac ccaggtcacc ccttcatcat gaccgtgggc tgcgtggctg 301 gtgatgagga gtcctacgaa gttttcaagg aactctttga ccccatcatc tcggatcgcc 361 acgggggcta caaacccact gacaagcaca agactgacct caaccatgaa aacctcaagg 421 gtggagacga cctggacccc aactacgtgc tcagcagccg cgtccgcact ggccgcagca 481 tcaagggcta cacgttgccc ccacactgct cccgtggcga gcgccgggcg gtggagaagc 541 tctctgtgga agctctcaac agcctgacgg gcgagttcaa agggaagtac taccctctga 601 agagcatgac ggagaaggag cagcagcagc tcatcgatga ccacttcctg ttcgacaagc 661 ccgtgtcccc gctgctgctg gcctcaggca tggcccgcga ctggcccgac gcccgtggca 721 tctggcacaa tgacaacaag agcctcctgg tgtgggtgaa cgaggaggat cacctccggg 781 tcatctccat ggagaagggg ggcaacatga aggaggtttt ccgccgcttc tgcgtagggc 841 tgcagaagat tgaggagatc tttaagaaag ctggccaccc cttcatgtgg aaccagcacc 901 TGGGCTACGT GCTCACCTGC CCATCCAACC TGGGCACTGG GCTGCGTGGA GGCGTGCATG 961 TGAAGCTGGC GCACCTGAGC AAGCACCCCA AGTTCGAGGA GATCCTCACC CGCCTGCGTC 1021 TGCAGAAGAG GGGTACAGGT GGCGTGGACA CAGCTGCCGT GGGCTCAGTA TTTGACGTGT 1081 CCAACGCTGA TCGGCTGGGC TCGTCCGAAG TAGAACAGGT GCAGCTGGTG GTGGATGGTG 1141 TGAAGCTCAT GGTGGAAATG GAGAAGAAGT TGGAGAAAGG CCAGTCCATC GACGACATGA 1201 TCCCCGCCCA GAAGTGAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1261 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1321 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCTGCA ACGGTCGGCA 1381 CTGAATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt