Construct: ORF TRCN0000488892
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021096.1_s317c1
- DNA Barcode:
- GGCCCCTGCCTAATCCCCTTTAAG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RGR (5995)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000488892
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5995 | RGR | retinal G protein coupled r... | NM_002921.3 | 99.6% | 100% | 19C>T;27T>C;471C>T |
| 2 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012720.2 | 98.3% | 98.6% | (many diffs) |
| 3 | human | 5995 | RGR | retinal G protein coupled r... | NM_001012722.1 | 85.4% | 85.7% | (many diffs) |
| 4 | human | 5995 | RGR | retinal G protein coupled r... | XM_024448118.1 | 71.5% | 71.1% | (many diffs) |
| 5 | human | 5995 | RGR | retinal G protein coupled r... | XR_002957005.1 | 30.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 957
- ORF length:
- 885
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggcagag accagtgcct tgcccaccgg cttcggggag ctcgaggtgc 121 tggctgtggg gatggtgcta ctggtggaag ctctctccgg tctcagcctc aataccctga 181 ccatcttctc tttctgcaag accccggagc tgcggactcc ctgccaccta ctggtgctga 241 gcttggctct tgcggacagt gggatcagcc tgaatgccct cgttgcagcc acatccagcc 301 ttctccgtgt ctcccacagg cgctggccct acggctcgga cggctgccag gctcacggct 361 tccagggctt tgtgacagcg ttggccagca tctgcagcag tgcagccatc gcatgggggc 421 gttatcacca ctactgcacc cgtagccagc tggcctggaa ctcagccgtc tctctggtgc 481 tcttcgtgtg gctgtcttct gccttctggg cagctctgcc ccttctgggt tggggtcact 541 atgactatga gccactgggg acatgctgca ccctggacta ctccaagggG GACAGAAACT 601 TCACCAGCTT CCTCTTCACC ATGTCCTTCT TCAACTTCGC CATGCCCCTC TTCATCACGA 661 TCACTTCCTA CAGTCTCATG GAGCAGAAAC TGGGGAAGAG TGGCCATCTC CAGGTAAACA 721 CCACTCTGCC AGCAAGGACG CTGCTGCTCG GCTGGGGCCC CTATGCCATC CTGTATCTAT 781 ACGCAGTCAT CGCAGACGTG ACTTCCATCT CCCCCAAACT GCAGATGGTG CCCGCCCTCA 841 TTGCCAAAAT GGTGCCCACG ATCAATGCCA TCAACTATGC CCTGGGCAAT GAGATGGTCT 901 GCAGGGGAAT CTGGCAGTGC CTCTCACCGC AGAAGAGGGA GAAGGACCGA ACCAAGTGAG 961 ACCCAGCTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGAGGCCC CTGCCTAATC CCCTTTAAGA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt